Targeting antibodies to the cytoplasm

Marschall, Andrea L. J.; Frenzel, André; Schirrmann, Thomas; Schüngel, Manuela; Dübel, Stefan

doi:10.4161/mabs.3.1.14110

Cited by 98 publications

(115 citation statements)

References 211 publications

(231 reference statements)

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“…Although there have been reports on the successful application of intracellular antibodies, called intrabodies if expressed in the cytosol, the use of this technology is very limited due to the failure of many antibodies to fold correctly in the cytosol. [6][7][8] Various solutions for this problem have recently been provided by the use of various alternative scaffolds which fold correctly in the cytosol. [9][10][11][12] Correct folding of antibodies is assured only in the endoplasmic reticulum (ER).…”

Section: Introductionmentioning

confidence: 99%

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Delivery of antibodies to the cytosol

Marschall

Zhang

Frenzel

et al. 2014

mAbs

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101

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Section: Introductionmentioning

confidence: 99%

“…There have been numerous reports on successful protein delivery into cells, including antibodies, 6,16 but a systematic comparison is still missing. Reports on delivery of proteins by virus-like particles are promising, but not applicable to antibodies because this method requires cytosolic expression of the cargo protein.…”

Section: Introductionmentioning

confidence: 99%

Delivery of antibodies to the cytosol

Marschall

Zhang

Frenzel

et al. 2014

mAbs

Self Cite

101

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“…Therefore, intense research has been invested within recent decades to develop general methods to achieve this goal 11c, 104. In principle, intracellular functional antigen‐binding proteins can be obtained by transfecting cells, for example with the use of intrabodies that are optimized for intracellular expression 11b.…”

Section: Cellular Delivery Of Small Antigen‐binding Proteinsmentioning

confidence: 99%

Nanobodies: Chemical Functionalization Strategies and Intracellular Applications

et al. 2018

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Nanobodies can be seen as next‐generation tools for the recognition and modulation of antigens that are inaccessible to conventional antibodies. Due to their compact structure and high stability, nanobodies see frequent usage in basic research, and their chemical functionalization opens the way towards promising diagnostic and therapeutic applications. In this Review, central aspects of nanobody functionalization are presented, together with selected applications. While early conjugation strategies relied on the random modification of natural amino acids, more recent studies have focused on the site‐specific attachment of functional moieties. Such techniques include chemoenzymatic approaches, expressed protein ligation, and amber suppression in combination with bioorthogonal modification strategies. Recent applications range from sophisticated imaging and mass spectrometry to the delivery of nanobodies into living cells for the visualization and manipulation of intracellular antigens.

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“…Although cargo molecules have known to be primarily taken up by CPP via endocytosis, direct membrane penetration is also considered an additional mechanism of uptake. 12) A theoretical model for the interaction of human immunodeficiency virus (HIV) Tat peptide (a well-known CPP) with membrane suggests the transient pore formation.…”

Section: Discussionmentioning

confidence: 99%

Cellular Uptake of IgG Using Collagen-Like Cell-Penetrating Peptides

Masuda

Yamamoto

Koide

2016

Biological & Pharmaceutical Bulletin

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Cell-penetrating peptides (CPPs) are attractive tools for delivering macromolecules that have poor membrane permeability, such as antibodies, into cells. However, the major drawback of conventional CPPs is their instability in bodily fluids. We previously reported a novel CPP employing a collagen-like triple-helical structure that exhibited remarkable resistance against serum proteases. Herein, we report the delivery of full-length immunoglobulin G (IgG) antibody into cells using a triple-helical CPP. The CPP was conjugated to IgG via a one-pot reaction using 2-iminothiolane as a crosslinking reagent. The triple-helical CPP was less prone to being aggregated and neutralized by serum than was octaarginine, a conventional CPP. However, most of the conjugates were found to be entrapped in endosomes.

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Targeting antibodies to the cytoplasm

Cited by 98 publications

References 211 publications

Delivery of antibodies to the cytosol

Delivery of antibodies to the cytosol

Nanobodies: Chemical Functionalization Strategies and Intracellular Applications

Cellular Uptake of IgG Using Collagen-Like Cell-Penetrating Peptides

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