Targeting bioactive compounds in natural extracts - Development of a comprehensive workflow combining chemical and biological data

Ory, Lucie; Nazih, Hassan; Daoud, Sahar Aly; Mocquard, Julia; Bourjot, Mélanie; Margueritte, Laure; Delsuc, Marc-André; Bard, Jean‐Marie; Pouchus, Yves François; Bertrand, Samuel; Roullier, Catherine

doi:10.1016/j.aca.2019.04.038

Cited by 34 publications

(17 citation statements)

References 57 publications

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“…The content and chemical structure of these secondary metabolites are liable for their different biological abilities, such as antioxidant potential to inhibit enzymes involved in the pathogenesis of the different diseases, cytotoxicity, antimicrobial activity, etc. [8]. In order to facilitate their bioavailability and raise qualitative and quantitative composition of extracts, new techniques for isolation are developing extensively [9].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Sambucus nigra L. Biopotential as an Unused Natural Resource

et al. 2021

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An unbreakable relationship between plants, nutrition, and health has directed researchers to deeply investigate and characterize the biopotential and medicinal properties of traditional foods. The aim of this study is to analyze and compare the phytochemical composition and biological potential of plant extracts with the idea of defining the most potent extracts as a natural source of bioactive molecules and their application in different industries. We evaluated unused plant species Sambucus nigra L. for investigation of bioactivities as potential natural products. Extracts of fresh elderberry fruits were obtained by modern (microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE)) and traditional (maceration (MAC)) extraction techniques, using 50% ethanol (50% EtOH) and water (H2O) of different polarities. In analyzed extracts, rutin and chlorogenic acid were dominant compounds in both 50% EtOH and H2O extracts, while ursolic acid was identified in 50% EtOH extracts as a terpenic compound with notable concentration. Elderberry extracts were evaluated regarding antioxidant, neuroprotective, antityrosinase, and antidiabetic abilities: MAE extracts had the best overall activity, and in general, 50% EtOH extracts were more potent than water extracts. The correlation of the dominant compound—rutin with all biological activities, indicates the importance of its presence in elderberries. S. nigra fruits showed excellent biopotential and opened possibilities of creating new food products or remedies, which are not present on the market because elderberry extracts are an exceptional source of rutin, chlorogenic acid, and ursolic acid.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Sambucus nigra L. Biopotential as an Unused Natural Resource

et al. 2021

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“…The introduction of biochemometric analyses to natural products in extracts or fractions provides an opportunity to unravel potential multiple effects of multi-components and provide potential markers in various components [ 35 , 36 ]. To investigate antioxidant- and anti-inflammation-specific metabolites in P. nudicaule , their metabolites and bioactivity (NO, PGE2, and ROS) from the EtOH extract and EtOAc fractions were linked using Pearson’s correlation and compared to those of P. rhoeas .…”

Section: Resultsmentioning

confidence: 99%

Ethyl Acetate Fractions of Papaver rhoeas L. and Papaver nudicaule L. Exert Antioxidant and Anti-Inflammatory Activities

et al. 2021

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Abnormal inflammation and oxidative stress are involved in various diseases. Papaver rhoeas L. possesses various pharmacological activities, and a previously reported analysis of the anti-inflammatory effect of P. nudicaule ethanol extracts and alkaloid profiles of the plants suggest isoquinoline alkaloids as potential pharmacologically active compounds. Here, we investigated anti-inflammatory and antioxidant activities of ethyl acetate (EtOAc) fractions of P. nudicaule and P. rhoeas extracts in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. EtOAc fractions of P. nudicaule and P. rhoeas compared to their ethanol extracts showed less toxicity but more inhibitory activity against LPS-induced nitric oxide production. Moreover, EtOAc fractions lowered the LPS-induced production of proinflammatory molecules and cytokines and inhibited LPS-activated STAT3 and NF-κB, and additionally showed significant free radical scavenging activity and decreased LPS-induced reactive oxygen species and oxidized glutathione. EtOAc fractions of P. nudicaule increased the expression of HO-1, GCLC, NQO-1, and Nrf2 in LPS-stimulated cells and that of P. rhoeas enhanced NQO-1. Furthermore, metabolomic and biochemometric analyses of ethanol extracts and EtOAc fractions indicated that EtOAc fractions of P. nudicaule and P. rhoeas have potent anti-inflammatory and antioxidant activities, further suggesting that alkaloids in EtOAc fractions are potent active molecules of tested plants.

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“…Hence, isolation of the active compounds which exert the antimicrobial/antioxidant activity helps to eradicate the toxic compounds, which are not needed in such therapeutic mixtures. ough the process of fractionating and isolating these bioactive compounds are often expensive, energy, and time consuming [273]; bioassay-guided fractionation of active compound is advised to reduce the complication that might arise [176,177,198,213].…”

Section: Toxicity Effect Of Unrefined Crude Extractsmentioning

confidence: 99%

Promising Natural Products in Crop Protection and Food Preservation: Basis, Advances, and Future Prospects

Ogunnupebi

Oluyori

Dada

et al. 2020

International Journal of Agronomy

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The increase in demand for agricultural produce necessitates the continuous search for affordable, ecofriendly, readily available crop protectors, and food preservatives. Historically, the use of various chemicals was employed in controlling plant diseases and to maintain food quality. In the past few decades, several natural product-based alternatives have been discovered and projected as better alternatives to synthetic pesticides and other synthetic agrochemicals. Recent studies focusing on the application of different botanicals in crop protection and food preservation were carefully selected and reviewed. The application of plant extract in the biogenic preparation of nanoparticles was also reviewed. This review confirms that several natural products can be used as a safe replacement for synthetic agrochemicals. Different plant extracts have also served as feed for the synthesis of nanoparticle, which is increasingly applicable in crop protection and food preservation.

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Targeting bioactive compounds in natural extracts - Development of a comprehensive workflow combining chemical and biological data

Cited by 34 publications

References 57 publications

Evaluation of Sambucus nigra L. Biopotential as an Unused Natural Resource

Evaluation of Sambucus nigra L. Biopotential as an Unused Natural Resource

Ethyl Acetate Fractions of Papaver rhoeas L. and Papaver nudicaule L. Exert Antioxidant and Anti-Inflammatory Activities

Promising Natural Products in Crop Protection and Food Preservation: Basis, Advances, and Future Prospects

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