Targeting CCL21–folic acid–upconversion nanoparticles conjugates to folate receptor-α expressing tumor cells in an endothelial-tumor cell bilayer model

Lee, Kim Yee; Seow, Eileen; Zhang, Yong; Lim, Yong Ching

doi:10.1016/j.biomaterials.2013.03.029

Cited by 46 publications

(29 citation statements)

References 38 publications

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“…This result was further validated by incubating HeLa cells, human lung carcinoma A549 cells and human umbilical vein endothelial HUVEC cells with GAI@CP. Because of the low- or no-expression of FR on membrane of A549 cells 37 or HUVEC cells 38, the cell viability of these two groups were 52.55% and 99.47%, respectively, while GAI@CP group killed 63.15% of HeLa cells in the same condition (Figure 3b), indicating the therapeutic selectivity of GAI@CP to FR high-expressed tumor cells.…”

Section: Resultsmentioning

confidence: 98%

A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting

Luo¹,

Huang²,

Yang³

et al. 2017

Theranostics

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To achieve the best therapeutic efficacy and good prognosis, the drugs necessitate tailored profiles of excellent spatiotemporal control and therapeutic monitoring. Here we introduce a programmed theranostic nanoparticle with self-adapting properties for tumor-specific systemic treatment, including stealthy surface to prolong circulation time in blood, surface charge-reversion for tumor targeting, receptor-mediated internalization to increase intracellular accumulation, “proton sponge effect” for controllable drug release and escape from endo/lysosome. Encouragingly, in the process of drug-induced apoptosis, the therapeutic efficacy can be reported by fluorescence imaging in vivo, in situ and in real time. Therefore, this work provides a new paradigm for design of programmed theranositc nanomedicine and offers promising prospects for precise tumor treatment.

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Section: Resultsmentioning

confidence: 98%

A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting

Luo¹,

Huang²,

Yang³

et al. 2017

Theranostics

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“…[43][44][45] In order to evaluate the cytotoxicity of the resulting nanoparticles, the viability of human breast cancer MCF-7 cells was tested by MTS assay in the presence of Fe 3 Prussian blue staining and ICP-OES analysis were conducted to determine whether the functionalized core/shell nanocomposites could be delivered into the MCF-7 cells in vitro ( Figure 10). Blue-stained iron can be clearly observed Figure 12A).…”

Section: Results and Discussion Synthesis And Characterization Of Fe mentioning

confidence: 99%

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Li¹,

Shen²,

Chen³

et al. 2015

IJN

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Engineering a safe and high-efficiency delivery system for efficient RNA interference is critical for successful gene therapy. In this study, we designed a novel nanocarrier system of polyethyleneimine (PEI)-modified Fe 3 O 4 @SiO 2 , which allows high efficient loading of VEGF small hairpin (sh)RNA to form Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites for VEGF gene silencing as well as magnetic resonance (MR) imaging. The size, morphology, particle stability, magnetic properties, and gene-binding capacity and protection were determined. Low cytotoxicity and hemolyticity against human red blood cells showed the excellent biocompatibility of the multifunctional nanocomposites, and also no significant coagulation was observed. The nanocomposites maintain their superparamagnetic property at room temperature and no appreciable change in magnetism, even after PEI modification. The qualitative and quantitative analysis of cellular internalization into MCF-7 human breast cancer cells by Prussian blue staining and inductively coupled plasma atomic emission spectroscopy analysis, respectively, demonstrated that the Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites could be easily internalized by MCF-7 cells, and they exhibited significant inhibition of VEGF gene expression. Furthermore, the MR cellular images showed that the superparamagnetic iron oxide core of our Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites could also act as a T 2 -weighted contrast agent for cancer MR imaging. Our data highlight multifunctional Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites as a potential platform for simultaneous gene delivery and MR cell imaging, which are promising as theranostic agents for cancer treatment and diagnosis in the future.

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“…In the contrast test, the FA-conjugated nanoparticles showed specific targeting ability to FA receptors expressing an ovarian carcinoma cell line, and exhibited no significant cytotoxicity effects. 23 Furthermore, based on the FA endowing tumor-targeting properties, a large number of FA-conjugated nanosystems have also been exploited for specific tumor diagnosis and imaging. [24][25][26][27] With the synergistic effects of nanosizemediated passive target and FA-mediated positive target, FA-modified nanocarriers are expected to be a great candidate for improving antitumor efficiency and biocompatibility for potential clinic usage.…”

Section: Chen Et Almentioning

confidence: 99%

Dual tumor-targeted poly(lactic-<em>co</em>-glycolic acid)–polyethylene glycol–folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery

Chen¹,

Wu²,

Luo³

et al. 2017

IJN

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Further specific target-ability development of biodegradable nanocarriers is extremely important to promote their security and efficiency in antitumor drug-delivery applications. In this study, a facilely prepared poly(lactic- co -glycolic acid) (PLGA)–polyethylene glycol (PEG)–folic acid (FA) copolymer was able to self-assemble into nanoparticles with favorable hydrodynamic diameters of around 100 nm and negative surface charge in aqueous solution, which was expected to enhance intracellular antitumor drug delivery by advanced dual tumor-target effects, ie, enhanced permeability and retention induced the passive target, and FA mediated the positive target. Fluorescence-activated cell-sorting and confocal laser-scanning microscopy results confirmed that doxorubicin (model drug) loaded into PLGA-PEG-FA nanoparticles was able to be delivered efficiently into tumor cells and accumulated at nuclei. In addition, all hemolysis, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, and zebrafish-development experiments demonstrated that PLGA-PEG-FA nanoparticles were biocompatible and secure for biomedical applications, even at high polymer concentration (0.1 mg/mL), both in vitro and in vivo. Therefore, PLGA-PEG-FA nanoparticles provide a feasible controlled-release platform for secure and efficient antitumor drug delivery.

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Targeting CCL21–folic acid–upconversion nanoparticles conjugates to folate receptor-α expressing tumor cells in an endothelial-tumor cell bilayer model

Cited by 46 publications

References 38 publications

A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting

A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Dual tumor-targeted poly(lactic-<em>co</em>-glycolic acid)–polyethylene glycol–folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery

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Targeting CCL21–folic acid–upconversion nanoparticles conjugates to folate receptor-α expressing tumor cells in an endothelial-tumor cell bilayer model

Cited by 46 publications

References 38 publications

A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting

A Programmed Nanoparticle with Self-Adapting for Accurate Cancer Cell Eradication and Therapeutic Self-Reporting

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Dual tumor-targeted poly(lactic-<em>co</em>-glycolic acid)&ndash;polyethylene glycol&ndash;folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery

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Dual tumor-targeted poly(lactic-<em>co</em>-glycolic acid)–polyethylene glycol–folic acid nanoparticles: a novel biodegradable nanocarrier for secure and efficient antitumor drug delivery