Targeting effect of microRNA on CD133 and its impact analysis on proliferation and invasion of glioma cells

Abstract

ABSTRACT. , a member of the microRNA-200 family, has been identified to be capable of suppressing glioma cell growth through targeting CREB1 or CD133. However, whether miR-200b affects the biological behavior (proliferation, invasion, and migration) of glioma cells is poorly understood. The aim of this study was to evaluate the effect of miR-200b on the biological behavior of glioma cells in vitro. inhibitor, and mimic control were transfected into conventionally cultured glioma U251 cells, followed by measuring the expression of miR-200b and CD133 in transfected cells by RT-PCR; effect of miR-200b on CD133 mRNA 3'-UTR luciferase activity by luciferase reporter assay; proliferation activity of transfected U251 cells by MTT method; and changes in U251 cell invasion and migration by Transwell method after transfection. Compared to that in the miRNA-200b inhibitor, mimic control, and blank control groups, miRNA-200b expression was significantly increased and CD133 mRNA expression was significantly decreased in the mimic miRNA200b group in a time-dependent manner (P < 0.05). Meanwhile, dual luciferase reporter assay showed that miR-200b could inhibit CD133 activity through binding to the 3'-UTR of CD133 mRNA (P < 0.05). Furthermore, the proliferation activity and invasion and migration abilities of U251 cells transfected with miRNA-200b mimic were significantly decreased (P < 0.05). In conclusion, overexpression of miR-200b inhibited the proliferation, invasion, and migration of glioma cells possibly through targeting CD133.

MicroRNA-200b reduced ZEB1 to inhibit cell proliferation and migration in human gastric cancer

“…And miR-200b is proved widely expressed in many tumors, including breast cancer, pancreatic cancer and GC [20]. Interestingly, miRNA-200b could inhibit the proliferation, invasion, and migration of glioma cells through targeting CD133 in glioma [21]. Moreover, Chen [22] has proved miRNA-200 could inhibit the growth of melanoma cells.…”

The miR-200 family: multiple effects on gliomas

“… 16 Similarly, many studies have demonstrated that miR-200b inhibits glioma cell proliferation and invasion. More specifically, miR-200b can target different genes, including cAMP responsive element-binding protein 1 (CREB1), zinc finger E-box binding homeobox 2 (ZEB2), prominin 1 (PROM1), extracellular signal-regulated kinase 5 (ERK5), CD133 10 , 19 , 31 – 33 and lactate dehydrogenase A (LDHA), which is associated with glioma cell proliferation and invasion. Therefore, targeting LDHA by miR-200b is regarded as a promising therapeutic strategy in glioma.…”

“… 9 It has been reported that miRNAs regulate cell growth associated with the development and metastasis of cancers. 10 Some miRNAs have been specifically implicated in glioma pathogenesis. For example, recent reviews have indicated that circulating miRNAs could be potential glioma biomarkers and reported that miRNAs are associated with drug resistance, which may have direct therapeutic implications.…”

Mathematical Modeling Regulatory Mechanisms of Hepatitis B Viruses’s Micro-Rna Action

“…Геном вирусов гепатита B кодирует микроРНК (miRNA). В последние годы наблюдается бурный рост работ по генной регуляции, связанный с открытием в геноме человека генов, кодирующих регуляторные молекулы -микроРНК, которые негативно регулируют экспрессию многих генов [1][2][3][4][5][6][7][8][9][10][11]. МикроРНК синтезируются из более длинных предшественников и не кодирует белки [1][2].…”