Targeting S. mutans biofilms: a perspective on preventing dental caries

Scharnow, Amber M.; Solinski, Amy E.; Wuest, William M.

doi:10.1039/c9md00015a

Cited by 71 publications

(58 citation statements)

References 111 publications

(122 reference statements)

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“…In contrast, immunoreactivity to P. gingivalis, yet another major pathogen in adult periodontitis [6], did not differ by age or time. Concerning caries-associated bacteria, immunoreactivity to S. mutans, B. dentium, and B. longum [18,26] increased significantly over time in both age groups, but with varying relation to age. Regrettably, we did not include Scardovia wiggsiae, an emerging caries "pathogen" in the test panel.…”

Section: Discussionmentioning

confidence: 95%

43-Year Temporal Trends in Immune Response to Oral Bacteria in a Swedish Population

et al. 2020

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Bacteria colonizing the mouth induce an adaptive immune response with the systemic and local presence of species or strain-specific immunoglobulins. Few studies have addressed global antibody patterns for oral bacteria or potential population time trends. We assessed these aspects in relation to a panel of oral bacteria. Using multiplex immunoblotting, IgG levels for 26 oral bacterial species (54 strains) were determined in 888 plasma samples from 30-year-old early pregnant women (n = 516) and 50-year-old men and women (n = 372) collected between 1976 and 2018. Inter-species correlations were found and age-dependent profiles and levels of immune responses to oral bacteria confirmed. We found temporal trends in the global and single-species antibody responses, but this was age-specific with both inclining and declining shifts. Prominent shifts in the younger group increased IgG towards health-associated Streptococcus salivarius and Streptococcus sanguinis, and in the older group towards disease-associated Aggregatibacter actinomycetemcomitans, Filifactor alocis, and Streptococcus mutans, among others. We concluded that temporal shifts occurred from 1976 to 2018, which may reflect improved oral health (more remaining teeth) and altered lifestyle habits, but this needs to be evaluated in observational studies considering more aspects.

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Section: Discussionmentioning

confidence: 95%

43-Year Temporal Trends in Immune Response to Oral Bacteria in a Swedish Population

et al. 2020

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“…There are multiple chemical strategies that could limit the development of dental biofilm, however, most of them can have side effects on teeth, soft tissues or killing oral microbiota, which show the need for specific therapies for cariogenic bacteria. Several studies have focused on blocking two important mechanisms for S. mutans biofilm development, such as avoiding sucrose-dependent or sucrose-independent adhesion and interference of cellular signaling "Quorum sensing" [38]. This study was carried out avoiding the sucrose-independent adhesion way, which has been aimed mainly at blocking sortase A, a transpeptidase involved at the anchoring of cell surface proteins, including Ag I/II, through the LPXTG motif.…”

Section: Adhesion Assaysmentioning

confidence: 99%

In silico Selection and in vitro Evaluation of Novel Inhibitors of Streptococcus Mutans Ag I/II Surface Protein

Cardona¹,

Padilla²,

Rivera³

et al. 2020

Preprint

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Streptococcus mutans is well known for having virulence factors associated with its cariogenic role, such as glucosyltransferases, which have been used as targets for the virtual screening of molecules with inhibitory capacity. The Antigen I/II of S. mutans is involved in the adhesion to the surface of the tooth and the bacterial co-aggregation in the biofilm formation, despite that, this protein has not been used as a target in a virtual strategy search for inhibitors. In this study we identified in silico and evaluated in vitro molecules with adhesion inhibitory potential on S. mutans Ag I/II. A virtual screening of 883,551 molecules was conducted, cytotoxicity analysis on fibroblast cells, S. mutans adhesion studies, scanning electron microscopy analysis for bacterial integrity, and molecular dynamics simulation were also performed. We have found three molecules (ZI-187, ZI-939, ZI-906) that were not cytotoxic and inhibited the adhesion of S. mutans to polystyrene microplates. Molecular dynamic simulation by 300 nanoseconds showed stability of the interaction between ZI-187 and Ag I/II (PDB: 3IPK). This work provides three new molecules that targets Ag I/II and have the capacity to inhibit in vitro the S. mutans adhesion on polystyrene microplates and provides a new computational line for the search and selection of safe inhibitory molecules against different pathogens.

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“…Adhesins on the surface of bacteria, including appendages and proteins, play a key role in the initial adhesion of bacteria to a substrate . In an independent pathway, Streptococcus mutans binds to salivary pellicles through cell surface adhesins, such as antigen I/II, SpaP, and Gbps …”

Section: Antibiofilm Mechanisms Of Nanoparticles At Different Stagesmentioning

confidence: 99%

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

Wang

Yuan

et al. 2019

Adv Healthcare Materials

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Due to their excellent size, designability, and outstanding targeted antibacterial effects, nanoparticles have become a potential option for controlling oral biofilm‐related infections. However, the formation of an oral biofilm is a dynamic process, and factors affecting the performance of antibiofilm treatments are complex. As such, when examining the existing literature on the antibiofilm effects of nanoparticles, attention should be paid to the specific mechanisms of action at different stages of oral biofilm formation, as well as relevant influencing factors, in order to achieve an objective and comprehensive evaluation. This review is intended to detail the antibacterial mechanisms of nanoparticles during the four stages of the formation of oral biofilms: 1) acquired film formation; 2) bacterial adhesion; 3) early biofilm development; and 4) biofilm maturation. In addition, factors influencing the antibiofilm properties of nanoparticles are summarized from the aspects of nanoparticles themselves, biofilm models, and host factors. The limitations of current research and possible trends for future research are also discussed. In summary, nanoparticles are a promising antioral biofilm strategy. It is hoped that this review can serve as a reference and inspire ideas for further research on the application of nanoparticles for effectively targeting and treating oral biofilms.

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Targeting S. mutans biofilms: a perspective on preventing dental caries

Abstract: This review aims to provide an overview of the current small molecule strategies used for targeting S. mutans biofilms, and a perspective of the future for the field.

Cited by 71 publications

References 111 publications

43-Year Temporal Trends in Immune Response to Oral Bacteria in a Swedish Population

43-Year Temporal Trends in Immune Response to Oral Bacteria in a Swedish Population

<em>In silico</em> Selection and <em>in vitro</em> Evaluation of Novel Inhibitors of <em>Streptococcus Mutans</em> Ag I/II Surface Protein

Nanoparticles for the Treatment of Oral Biofilms: Current State, Mechanisms, Influencing Factors, and Prospects

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