Targeting of Mitochondria-Endoplasmic Reticulum by Fluorescent Macrocyclic Compounds

Cruz, Carla; Cairrão, Elisa; Silvestre, Samuel; Breitenfeld, Luiza; Almeida, Paulo; Queiroz, João A.

doi:10.1371/journal.pone.0027078

Cited by 16 publications

(17 citation statements)

References 37 publications

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“…[221] In 2010, the Che research group developed the new ER reagent 111,w hich consists of ac ytotoxic artemisinin conjugated to af luorescent dansyl moiety (Scheme 39). [221] In 2010, the Che research group developed the new ER reagent 111,w hich consists of ac ytotoxic artemisinin conjugated to af luorescent dansyl moiety (Scheme 39).…”

Section: Er-targeting Activatable Fluorescent Probesmentioning

confidence: 99%

“…Selective ER reagents share common features with the ER trackers:t hey are amphipathic,l ipophilic cationic dyes with moderate-sized conjugated systems (e.g.D iOC 6 (108), Hexyl Rhodamine B( 109), and ER-Tracker Bluewhite DPX (110), Scheme 39). [221] In 2010, the Che research group developed the new ER reagent 111,w hich consists of ac ytotoxic artemisinin conjugated to af luorescent dansyl moiety (Scheme 39). [222] Thep erinuclear and reticulate fluorescent imaging indicated that this probe located at the ER.…”

Section: Er-targeting Activatable Fluorescent Probesmentioning

confidence: 99%

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Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

et al. 2016

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Principle has it that even the most advanced super-resolution microscope would be futile in providing biological insight into subcellular matrices without well-designed fluorescent tags/probes. Developments in biology have increasingly been boosted by advances of chemistry, with one prominent example being small-molecule fluorescent probes that not only allow cellular-level imaging, but also subcellular imaging. A majority, if not all, of the chemical/biological events take place inside cellular organelles, and researchers have been shifting their attention towards these substructures with the help of fluorescence techniques. This Review summarizes the existing fluorescent probes that target chemical/biological events within a single organelle. More importantly, organelle-anchoring strategies are described and emphasized to inspire the design of new generations of fluorescent probes, before concluding with future prospects on the possible further development of chemical biology.

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Section: Er-targeting Activatable Fluorescent Probesmentioning

confidence: 99%

Section: Er-targeting Activatable Fluorescent Probesmentioning

confidence: 99%

Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

et al. 2016

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“…Moreover, it is also important to establish the relationships between the compound’s structure, its localization and its effects on cell death as it should or could be related. There are a number of structural aspects that seems to affect the intracellular distribution of the compounds: the nature of chromophore, total charge of the compounds, the presence of secondary and tertiary amines, pendant side arms and counter ion of the compound could influence the sub‐cellular distribution (26,27). Generally, anticancer drugs are effective against tumors depending on the molecular target of the drug.…”

Section: Discussionmentioning

confidence: 99%

Polyazamacrocycles as Potential Antitumor Agents for Human Prostate Cancer Cells

et al. 2013

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Polyazamacrocycles are currently being studied and used in a variety of applications beyond their traditional place in supramolecular and co-ordination chemistry. This study suggests additional applications of these compounds with particular emphasis on their use as antiproliferative agents that could be potentially used to treat cancer. Four polyazamacrocycles were tested in human prostate cancer LNCaP and prostate epithelial PNTA1 cells to analyze changes in cell proliferation and cell death capabilities. Their intracellular localization was also evaluated by confocal microscopy. The results show a decrease in proliferation rate and cell viability of LNCaP and PNTA1, after treatment with these compounds. The decrease in the number of viable cells is similar for the majority of the compounds studied, and at higher concentration, the proliferation efficiency decreased significantly in the cell lines studied. Also, our results suggest that L and L2 induce early apoptosis in PNTA1 cells and late apoptosis/necrosis in LNCaP cells. The compounds did not induce a significant increase in necrosis of both cell types. Although the compounds did not localize in a unique organelle, all of them have as main target the Golgi apparatus and other localization profiles differed depending on the cell line.

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“…Then, 200 μL of the mixture above mentioned was removed and absorbance was recorded in microplate reader at 570 nm. [ 14 ] The experiment was carried out in triplicate and the extent of cell death was expressed as the percentage of cell viability in comparison with control cells.…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts

Luís¹,

Breitenfeld²,

Ferreira³

et al. 2014

Phcog Mag

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Background:Hakea sericea Schrader is an invasive shrub in Portuguese forests.Objective:The goal of this work was to evaluate the antimicrobial activity of H. sericea extracts against several strains of microorganisms, including the ability to inhibit the formation of biofilms. Additionally the cytotoxic properties of these extracts, against human cells, were assessed.Materials and Methods:The antimicrobial activity of the methanolic extracts of H. sericea was assessed by disk diffusion assay and Minimum Inhibitory Concentration (MIC) value determination. The antibiofilm activity was determined by quantification of total biofilm biomass with crystal violet. Cytotoxicity was evaluated by hemolysis assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test.Results:For Gram-positive bacteria, MIC values of H. sericea methanolic extracts ranged between 0.040 and 0.625 mg/mL, whereas the fruits extract yielded the lowest MIC for several strains of microorganisms, namely, S. aureus, B. cereus, L. monocytogenes and clinical methicillin-resistant S. aureus (MRSA). Stems and fruits extract at 2.5 mg/mL effectively eradicated the biofilm of S. aureus ATCC 25923, SA 01/10 and MRSA 12/10. Regarding leaves extract, hemolysis was not observed, and in the case of stems and fruits, hemolysis was verified only for higher concentrations, suggesting its low toxicity. Fruits extract presented no toxic effect to normal human dermal fibroblasts (NHDF) cells however for concentrations of 0.017 and 0.008 mg/mL this extract was able to decrease human breast adenocarcinoma cells (MCF-7) viability in about 60%, as MTT test results had confirmed. This is a clearly demonstrator of the cytotoxicity of this extract against MCF-7 cells.

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Targeting of Mitochondria-Endoplasmic Reticulum by Fluorescent Macrocyclic Compounds

Cited by 16 publications

References 37 publications

Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

Discerning the Chemistry in Individual Organelles with Small‐Molecule Fluorescent Probes

Polyazamacrocycles as Potential Antitumor Agents for Human Prostate Cancer Cells

Antimicrobial, antibiofilm and cytotoxic activities of Hakea sericea Schrader extracts

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