Targeting of sebaceous glands by δ‐aminolevulinic acid‐based photodynamic therapy: An in vivo study

Kosaka, Sachiko; Miyoshi, Norio; Akilov, Oleg E.; Hasan, Tayyaba; Kawana, Seiji

doi:10.1002/lsm.21062

Cited by 20 publications

(12 citation statements)

References 22 publications

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“…18,19 This mechanism could be applied to porphyrins, which are important molecules for PDT photosensitizers. 1,20,21 Electrondonor-connecting cationic porphyrins have been synthesized to examine the activity control during interaction with DNA. 22,23 The OFF → ON activity controls of pyrene 22 -and anthracene 23 -connecting porphyrins by DNA were successfully established.…”

Section: ■ Introductionmentioning

confidence: 99%

Relaxation Process of Photoexcited meso-Naphthylporphyrins while Interacting with DNA and Singlet Oxygen Generation

2015

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Electron donor-connecting cationic porphyrins meso-(1-naphthyl)-tris(N-methyl-p-pyridinio)porphyrin (1-NapTMPyP) and meso-(2-naphthyl)-tris(N-methyl-p-pyridinio)porphyrin (2-NapTMPyP) were designed and synthesized. DFT calculations speculate that the photoexcited states of 1- and 2-NapTMPyPs can be deactivated via intramolecular electron transfer from the naphthyl moiety to the porphyrin moiety. However, the quenching effect through the intramolecular electron transfer is insufficient, possibly due to the orthogonal position of the electron donor and the porphyrin ring and the relatively small driving force: Gibbs energies are 0.11 and 0.07 eV for 1- and 2-NapTMPyPs, respectively. It was speculated that more than 0.3 eV of the driving force is required to realize effective electron transfer in similar electron-donor connecting porphyrin systems. These porphyrins aggregated around the DNA strand, accelerating the deactivation of their excited singlet state and decreasing their photosensitized singlet oxygen-generating activities. In the presence of a sufficiently large concentration of DNA, these porphyrins can bind to a DNA strand stably, leading to an increased fluorescence quantum yield and lifetime. Singlet oxygen generation was also suppressed by the aggregation of porphyrins around DNA. Although the quantum yield of singlet oxygen generation was recovered in the presence of sufficient DNA, the singlet oxygen generated by DNA-binding porphyrins was significantly smaller than that without DNA. These results suggest that DNA-binding drugs limit the generation of photosensitized singlet oxygen by quenching the DNA strand.

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Section: ■ Introductionmentioning

confidence: 99%

Relaxation Process of Photoexcited meso-Naphthylporphyrins while Interacting with DNA and Singlet Oxygen Generation

2015

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“…Interestingly, a recent study has shown that sebaceous glandtargeted accumulation of ALA-PpIX and distinctive damage to sebaceous glands by PDT still can be achieved by shortening the incubation time and decreasing the ALA concentration [27]. All patients tolerated the treatment without application of topical anesthesia and showed no serious side effects such as acneiform eruption, scarring or infection, except for transient erythema, exfoliation, and hyperpigmentation in four of 15 patients.…”

Section: Discussionmentioning

confidence: 86%

Photodynamic therapy using 5-aminolevulinic acid and intense pulsed light against intense pulsed light alone in the treatment of acne vulgaris

Ragab¹,

Hussein²,

Salem³

2014

Journal of the Egyptian Womenʼs Dermatologic Society

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BackgroundPhotodynamic therapy (PDT) is one of the promising new modalities for the treatment of acne vulgaris. Objective The aim of this study was to compare the efficacy and safety of PDT using 5-aminolevulinic acid (ALA) with intense pulsed light (IPL) and IPL alone in the treatment of mild to moderate acne vulgaris. Patients and methodsThis was a randomized controlled clinical trial. Twenty-five patients of both sexes with mild to moderate facial acne participated in this study. The patients were divided into two groups: group I included 15 patients who received ALA-PDT using IPL and group II included 10 patients who were treated with IPL only. Both groups received two sessions at 2-week interval. The patients were evaluated clinically at baseline and on the second and the eighth weeks after the last treatment. ResultsThe ALA-PDT group showed significant improvement in treating both inflammatory and comedonal lesions compared with at baseline. In addition, the reduction was greater for inflammatory lesions than for comedonal lesions (73.4 vs. 33.6%). The IPL-only group showed an 18.9% reduction in inflammatory lesions and a 29.8% reduction in comedones. The mean percent reduction from baseline to the end point of treatment in inflammatory lesions was significantly greater in the PDT-IPL group than in the IPL-only group (P = 0.012). Side effects were minimal. Conclusion Analysis of the results revealed that both ALA-PDT with IPL as a light source and IPL alone are efficient and safe treatment modalities for acne vulgaris with superiority of ALA-PDT in reduction of inflammatory lesions.

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“…First, efficacy of continuous application of low‐dose photosensitizers has some scientific validity. One group reported that satisfactory anti‐inflammatory effects around sebaceous glands can be achieved with ALA‐PDT with no prominent damage in the epidermis with low concentrations of ALA (2.5–5.0%) applied in the mouse in vivo . Researchers also recently demonstrated that application of 5% ALA was clinically effective for Asian acne patients without notable side‐effects .…”

Section: Discussionmentioning

confidence: 99%

Daylight photodynamic therapy with 1.5% 3‐butenyl 5‐aminolevulinate gel as a convenient, effective and safe therapy in acne treatment: A double‐blind randomized controlled trial

Kwon

Moon

Park

et al. 2015

The Journal of Dermatology

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While daylight photodynamic therapy (PDT) is a simpler and more tolerable treatment procedure for both clinicians and patients, it has never been applied for acne treatment. In this study, we evaluated efficacy, safety and histological changes of facial acne after application of the novel variant of 5-aminolevulinate (ALA)-ester, 1.5% 3-butenyl ALA-bu gel, using daylight only as the potential visible light source. Forty-six acne patients were randomly assigned to either ALA-bu or vehicle application group in a double-blind fashion. Both groups applied the allocated gel to facial acne lesions every other day for 12 weeks. At the final 12 week, both inflammatory and non-inflammatory acne lesions had decreased significantly by 58.0% and 34.1% in the ALA-bu group, respectively. Only a few patients expressed mild adverse effects. In the histopathological analysis, attenuated inflammatory cell infiltrations were observed and immunostaining intensities for interleukin-8, interleukin-1β, matrix metalloproteinase-9 and phosphorylated nuclear factor-κB were reduced concomitantly. Changes of their mRNA expression demonstrated comparable patterns. In conclusion, this ambulatory PDT was effective, very well tolerated and convenient for treating inflammatory acne lesions. Experimental results correlated well with clinical results. This novel regimen would provide a viable option for acne therapy.

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Targeting of sebaceous glands by δ‐aminolevulinic acid‐based photodynamic therapy: An in vivo study

Abstract: Focused damage of sebaceous glands can be achieved with ALA-PDT when low concentrations of ALA (2.5-5.0%) and short incubation times (to 2 hours) were used.

Cited by 20 publications

References 22 publications

Relaxation Process of Photoexcited meso-Naphthylporphyrins while Interacting with DNA and Singlet Oxygen Generation

Relaxation Process of Photoexcited meso-Naphthylporphyrins while Interacting with DNA and Singlet Oxygen Generation

Photodynamic therapy using 5-aminolevulinic acid and intense pulsed light against intense pulsed light alone in the treatment of acne vulgaris

Daylight photodynamic therapy with 1.5% 3‐butenyl 5‐aminolevulinate gel as a convenient, effective and safe therapy in acne treatment: A double‐blind randomized controlled trial

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