Targeting REDV peptide functionalized polycationic gene carrier for enhancing the transfection and migration capability of human endothelial cells

Wang, Haixia; Feng, Yakai; Yang, Jing; Guo, Jintang; Zhang, Wencheng

doi:10.1039/c4tb02019g

Cited by 40 publications

(54 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chains of PEG could shield PEI and buffer the adsorption of polymers and cell membrane. 28 The increased PEG content enhanced the shielding effect greatly, thus decreasing the cytotoxicity. When the concentration was below 30 μg mL -1 , MPs and complexes showed perfect cell viability.…”

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

“…22 In our previous studies, several gene carriers to condense with pEGFP-ZNF580 gene (pEGFP-ZNF580 gene is the recombinant plasmid of enhanced GFP plasmid and ZNF580 gene) were prepared with different block copolymers for the proliferation of ECs. [23][24][25][26][27][28][29] As the "gold" standard nonviral gene carrier, polyethylenimine (PEI) is widely used as an effective gene carrier, which exhibits a good performance in condensing DNA due to its high positive charge density. 30 However, the drawbacks of high-molecular weight PEI, including nondegradability and high cytotoxicity, limit its application in vivo.…”

Section: Feng Et Almentioning

confidence: 99%

See 1 more Smart Citation

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Feng¹,

Guo²,

Li³

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

Section: In Vitro Cytotoxicitymentioning

confidence: 99%

Section: Feng Et Almentioning

confidence: 99%

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Feng¹,

Guo²,

Li³

et al. 2016

IJN

Self Cite

View full text Add to dashboard Cite

“…49 The selective adhesion of ECs over SMCs is attracting increasing attention for the use of REDV peptide in the surface modification of artificial vascular grafts and gene carriers. [50][51][52][53] In addition, other investigators have immobilized antibodies, growth factors, proteins and genes to improve the interaction of implants with ECs to accelerate endothelialization. 38,51,[54][55][56][57][58][59] Infections have become one of the main causes of patient morbidity and mortality.…”

Section: Introductionmentioning

confidence: 99%

“…[50][51][52][53] In addition, other investigators have immobilized antibodies, growth factors, proteins and genes to improve the interaction of implants with ECs to accelerate endothelialization. 38,51,[54][55][56][57][58][59] Infections have become one of the main causes of patient morbidity and mortality. 60,61 In particular, infections caused by surface-bound bacterial cells are a major problem in clinical surgeries and implantation operations.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelialization

et al. 2015

Self Cite

View full text Add to dashboard Cite

Polycarbonate urethane (PCU) elastomeric materials have been developed for vascular prosthesis applications, because of their excellent mechanical and physical properties. However, thrombosis and inflammation often limit their usage as small-diameter vascular grafts. Herein, we focused on the design and functionalization of a PCU elastomer with enhanced hemocompatibility, rapid endothelialization and antimicrobial properties. An atom transfer radical polymerization (ATRP) technique was utilized to graft random copolymers of N-(2-hydroxypropyl)methacrylamide (HPMA) and eugenyl methacrylate (EgMA) onto a PCU surface, and subsequently the cysteine-terminated CREDV peptide sequence was directly linked onto the surface by a thiol-ene click reaction to prepare a series of REDV peptide functionalized surfaces. The chemical compositions of the modified surfaces were quantified by X-ray photoelectron spectroscopy (XPS), and the hydrophilicity was evaluated by water contact analysis and water uptake.The surface hemocompatibility was verified by platelet adhesion assays, and the results demonstrated that platelet adhesion was significantly reduced on the modified surface. More importantly, the functionalized surfaces with high hydrophilicity and cell specific adhesive REDV peptide could selectively enhance the adhesion and proliferation of human umbilical vein endothelial cells (HUVECs) but they suppressed these behaviors in human arterial smooth muscle cells (HASMCs). Moreover, these surfaces showed excellent antibacterial properties, which originate from the EgMA moieties of the copolymers. The successful fabrication of multifunctional surfaces with excellent hemocompatibility, rapid endothelialization, and good antimicrobial activity through a feasible route could be an attractive platform for tissue engineering applications.

show abstract

“…The ideal materials for vascular tissue engineering should have excellent biocompatibility, good mechanical properties, and appropriate degradation behavior [23]. Biodegradable polymers, such as poly(L-lactic acid) (PLLA), poly(glycolic acid) (PGA) and poly(lactic-co-glycolic acid) (PLGA), have drawn a lot of attention in research because of their well-characterized biodegradable properties [24][25][26][27][28]. These materials have been successfully used in bone tissue, vessel, and patches for wounds [29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) Nanofibrous Scaffolds for Tissue Engineering

et al. 2016

Self Cite

View full text Add to dashboard Cite

Biomimetic scaffolds have been investigated in vascular tissue engineering for many years. Excellent biodegradable materials are desired as temporary scaffolds to support cell growth and disappear gradually with the progress of guided tissue regeneration. In the present paper, a series of biodegradable copolymers were synthesized and used to prepared micro/nanofibrous scaffolds for vascular tissue engineering. Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) [P(LA-co-GA-co-MMD)] copolymers with different L-lactide (LA), glycolide (GA), and 3(S)-methyl-2,5-morpholinedione (MMD) contents were synthesized using stannous octoate as a catalyst. Moreover, the P(LA-co-GA-co-MMD) nanofibrous scaffolds were prepared by electrospinning technology. The morphology of scaffolds was analyzed by scanning electron microscopy (SEM), and the results showed that the fibers are smooth, regular, and randomly oriented with diameters of 700˘100 nm. The weight loss of scaffolds increased significantly with the increasing content of MMD, indicating good biodegradable property of the scaffolds. In addition, the cytocompatibility of electrospun nanofibrous scaffolds was tested by human umbilical vein endothelial cells. It is demonstrated that the cells could attach and proliferate well on P(LA-co-GA-co-MMD) scaffolds and, consequently, form a cell monolayer fully covering on the scaffold surface. Furthermore, the P(LA-co-GA-co-MMD) scaffolds benefit to excellent cell infiltration after subcutaneous implantation. These results indicated that the P(LA-co-GA-co-MMD) nanofibrous scaffolds could be potential candidates for vascular tissue engineering.

show abstract

Targeting REDV peptide functionalized polycationic gene carrier for enhancing the transfection and migration capability of human endothelial cells

Abstract: Targeting REDV peptide functionalized polycationic gene carrier for enhancing the transfection and migration capability of human endothelial cells.

Cited by 40 publications

References 51 publications

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Co-self-assembly of cationic microparticles to deliver pEGFP-ZNF580 for promoting the transfection and migration of endothelial cells

Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelialization

Electrospun Poly(lactide-co-glycolide-co-3(S)-methyl-morpholine-2,5-dione) Nanofibrous Scaffolds for Tissue Engineering

Contact Info

Product

Resources

About