Targets of the cyclin-dependent kinase Cdk1

Abstract

The events of cell reproduction are governed by oscillations in the activities of cyclin-dependent kinases (Cdks). Cdks control the cell cycle by catalysing the transfer of phosphate from ATP to specific protein substrates. Despite their importance in cell-cycle control, few Cdk substrates have been identified. Here, we screened a budding yeast proteomic library for proteins that are directly phosphorylated by Cdk1 in whole-cell extracts. We identified about 200 Cdk1 substrates, several of which are phosphorylated in vivo in a Cdk1-dependent manner. The identities of these substrates reveal that Cdk1 employs a global regulatory strategy involving phosphorylation of other regulatory molecules as well as phosphorylation of the molecular machines that drive cell-cycle events. Detailed analysis of these substrates is likely to yield important insights into cell-cycle regulation.

Yeast and fungal morphogenesis from an evolutionary perspective

“…Recent work from several labs suggests that the GAPs are important targets for cell cycle kinases to control the timing of polarization (Figure 1). Two proteomics searches for proteins associated with specific Cdc28-cyclin complexes identified Rga1 and Bem3 as targets of Cdc28 in complex with G1 cyclin Gln2 [92,93]. Three recent reports involving S. cerevisae and one involving C. albicans provided evidence that the GAPs are phosphorylated by Cdc28 and Pho85 Cdks in late G1 [90,[94][95][96].…”

Pil1 Controls Eisosome Biogenesis

“…The Cdc28 mutant strain (KEM100) was generated by integrating the plasmid pRS406 carrying the cdc28-as2 allele in the URA locus of TWY110. The cdc28-as2 plasmid was generated by David Morgan's laboratory at the University of California, San Francisco (Ubersax et al, 2003) and was a generous gift from Gustavo Pesce (Molecular Sciences Institute, Berkeley, CA). The copper inducible strains KEM102 and KEM103 were made by replacing the endogenous promoter of PIL1 with the CUP1 promoter in TWY110 and TWY113.…”

Fine regulation of Saccharomyces cerevisiae MAPK pathways by post‐translational modifications

“…Interestingly, global phosphoproteomic studies aimed to identify CDK-dependent phosphorylation events [31,57] have unveiled some putative protein targets related to MAPK signalling. For example, Spa2, a polarisome component considered as a scaffold for the CWI pathway during vegetative growth [11], as well as other proteins of the polarisome (Pea2 and Bud6), are putative CDK targets.…”