Tarsal Conjunctiva of the Upper Eyelid

Dark, A. J.; Durrant, Tim; McGinty, F.; Shortland, J. R.

doi:10.1016/0002-9394(74)90471-1

Cited by 24 publications

(14 citation statements)

References 20 publications

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“…Several authors have proposed that this co-expression in Squamous epithelial cells may be indicative of proliferative activity [16,17]. In this regard, it must be borne in mind that our primary cultures came from the fornical area of the conjunctiva, the proposed location of conjunctival stem cells [6,36,37].…”

Section: Discussionmentioning

confidence: 99%

Characterization of epithelial primary cultures from human conjunctiva

Diebold

Calonge

Fernández

et al. 1997

Graefe's Arch Clin Exp Ophthalmol

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Primary cultures of human epithelial cells from normal conjunctiva were developed and characterized to determine whether they retained epithelial characteristics. Conjunctival explants were obtained from the upper fornix of healthy donors and cultured in supplemented DMEM/F-12 medium for 5 days. The epithelial outgrowth was maintained for an additional 10 days. Primary cultures were then processed for light microscopy, transmission and scanning electron microscopy (TEM, SEM), and immunocytochemistry. They exhibited typical features of conjunctival epithelium on light microscopy (polygonal morphology, intimate cohesion, production of mucins), TEM (abundant desmosomes, keratin bundles, granules, microvilli), SEM (polygonal shape, microvilli, intimate cohesion), and immunocytochemistry (positivity for the receptor of epidermal growth factor, desmosomal proteins, and cytokeratins). In conclusion, primary cultures developed from normal human conjunctiva maintained the epithelial characteristics in vitro. Because the conjunctiva is a major component of the anterior ocular surface, we propose this in vitro system as suitable for physiopathologic and toxicologic studies.

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Section: Discussionmentioning

confidence: 99%

Characterization of epithelial primary cultures from human conjunctiva

Diebold

Calonge

Fernández

et al. 1997

Graefe's Arch Clin Exp Ophthalmol

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“…From the bibliographic analysis, controversial data were obtained on this topic. In fact, either in normal [1,7,8,14] or in pathological [10,20] human eyes or in animal species, such as the dog [11] and the rabbit [10], a uniform layer of microvilli or a double population of microvilli and microplicae were described on the conjunctival surface. As conflicting results on the presence of microvilli and microplicae are also reported regarding the corneal surface [12], we demonstrated that in the Mongolian gerbil conjunctiva only microplicae were detectable: as to their role, they could provide a better adhesion of mucin to the epithelium [3].…”

Section: Discussionmentioning

confidence: 99%

“…The conjunctival epithelium partially covers the external ocular surface and takes part in the defense mechanisms of the ocular surface and in the production of the tear film [1]. These functions, in all vertebrates up to now studied [1][2][3][4][5][6], are provided in a fully developed conjunctiva by two well-distinguished main cellular types: the epithelial cells and the goblet cells.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural Study of the Conjunctival Epithelium in the Mongolian Gerbil (Meriones unguiculatus)

Micali

Puzzolo²,

Pisani³

et al. 1998

Ophthalmic Res

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The conjunctival epithelium of the Mongolian gerbil (Meriones unguiculatus) was studied using the transmission and scanning electron microscopy. The superficial cells of the conjunctival epithelium were isoprismatic in shape and were covered, on their luminal surface, with microplicae. They were connected with the adjacent cells by junctional complexes and desmosomes. Some of the superficial cells were partially or completely detached from the underlying intermediate cells: this morphological pattern was interpreted as an expression of cellular renewal of the conjunctival surface. Goblet cells were either isolated or gathered in clusters: in any case, they were firmly connected with the adjacent epithelial cells. The apical part of the goblet cells was covered with a great number of long microvilli: they showed a variety of morphological aspects, which were interpreted as occurring in a sequential pattern during the secretory process. The Mongolian gerbil can be considered among the laboratory animals used for ophthalmic research.

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“…A similar epithelium, that of the limbal zone, has been described by Hogan et al (1971). The surface morphology of the human palpebral/tarsaI conjunctiva was examined by Takakusaki (1969), Dark et al (1974) and Greiner et al (1977).…”

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confidence: 99%

The Ultrastructure of the Normal Conjunctival Epithelium of the Guinea Pig

Latkovic

Nilsson

1979

Acta Ophthalmologica

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The ultrastructure of the superficial cell layer of the perilimbal zone of the guinea pig conjunctival epithelium as seen in transmission and scanning electron micrographs is described. The major interest is focused on the apparatus of vesicles, vacuoles and cysts and their possible significance in connexion with transport functions and autophagocytic processes. The importance of the cell surface components and the role of the microfilaments in phagocytosis are also discussed.

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Tarsal Conjunctiva of the Upper Eyelid

Cited by 24 publications

References 20 publications

Characterization of epithelial primary cultures from human conjunctiva

Characterization of epithelial primary cultures from human conjunctiva

Ultrastructural Study of the Conjunctival Epithelium in the Mongolian Gerbil (Meriones unguiculatus)

The Ultrastructure of the Normal Conjunctival Epithelium of the Guinea Pig

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