Tartrate-resistant acid phosphatase as a diagnostic factor for arthritis

Abstract

Abstract. Tartrate-resistant acid phosphatase (TRAP) is highly expressed in osteoclasts and chondroclasts. The present study investigated changes in TRAP activity after chondrocyte death and cartilage damage, and also evaluated the possible use of TRAP as a diagnostic factor in a model of osteoarthritis. We induced experimental osteoarthritis in beagle dogs and separated chondrocytes from articular cartilage using an enzyme probe. Chondrocyte death was induced by proteasome inhibition and TRAIL treatment, and levels of lactate dehydrogenase, reactive oxygen species (ROS), caspase activation and TRAP activity were measured in the chondrocytes and synovial fluid. Proteasome inhibition and TRAIL treatment significantly enhanced chondrocyte death via caspase-8 activation and ROS generation in the primary cultured canine chondrocytes. TRAP activity was highly increased in damaged chondrocytes, but was decreased by blocking chondrocyte death using caspase inhibition or an ROS scavenger. In the synovial fluid of osteoarthritic dogs, TRAP activity as well as caspase activation and ROS levels were higher than those in the normal joint. Our study demonstrated that TRAP is activated by apoptosis and oxidative stress in primary cultured chondrocytes and osteoarthritic joints and also suggests that TRAP may be used as a diagnostic biomarker for detection of cartilage-related diseases, including osteoarthritis.

DiaPASEF proteotype analysis indicates changes in cell growth and metabolic switch induced by caspase‐9 inhibition in chondrogenic cells

“…Interestingly, the most downregulated protein was tartrateresistant acid phosphatase type 5 (PPA5). This marker of bone resorption is highly expressed in osteoclasts and chondroclasts, but PPA5 activity was also found in osteoathritic chondrocytes and as such, it was proposed as a diagnostic factor [39,40]. The level of PPA5 was decreased after general caspase inhibition [39], in agreement with our findings which further specify the impact of caspase-9 selective inhibition.…”

Bioactivity of benzophenazine enaminone derivatives as inhibitors of ERK2 based on molecular docking and dynamics simulation studies

“…These methods were employed to predict the binding affinity of the molecules to a protein known as ERK2, which is associated with skin cancer. [31][32][33][34] It should be noted that the selection of ERK2 as the target protein for investigating benzophenazine enaminone derivatives as inhibitors is grounded in its significance in cancer, suitability for drug targeting, prior research outcomes, and specificity. This choice represents a logical and scientifically justified strategy in the endeavor to develop potential therapeutic agents for treating cancer.…”

Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods

“…Acid phosphatase (ACP) is a phosphomonoesterase widely found in nature that can catalyze the hydrolysis of phosphomonoesters under acidic conditions in mammalian tissues [1], including the prostate, liver, and blood systems [2,3]. Although the ACP concentration is low in human cells, it involves many important physiological processes, especially mammalian movement [4].…”