Tartrate-resistant acid phosphatase in bone and cartilage following decalcification and cold-embedding in plastic.

Cole, Ada A.; Walters, Linda M.

doi:10.1177/35.2.3540104

Cited by 181 publications

(100 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…TRAP staining of the scales was performed by the methods of Cole and Walters [35]. After TRAP staining, the specimens were counterstained with 4', 6-diamino-2-phenylindole (DAPI) (Molecular Probes, Inc., Eugene, OR, USA).…”

Section: Effect Of Fugu Pth1(1-34) On the Differentiation Of Mononuclmentioning

confidence: 99%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

View full text Add to dashboard Cite

The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/ml to 10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/ml to 10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-B ligand in osteoblasts and the receptor activator NF-B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.3

show abstract

Section: Effect Of Fugu Pth1(1-34) On the Differentiation Of Mononuclmentioning

confidence: 99%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

View full text Add to dashboard Cite

show abstract

“…Subsequently, osteoclasts were observed by TRAP staining using the methods of Cole and Walters (1987). Osteoblasts were detected by alkaline phosphatase (ALP) staining using a kit (NBT/BCIP Stock Solution, Roche Applied Science, Mannheim, Germany).…”

Section: Morphological Study Of Osteoclasts and Osteoblasts In The Scmentioning

confidence: 99%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

View full text Add to dashboard Cite

To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

show abstract

“…1 Clusters of tumor cells stained strongly positively for tartrate-resistant acid phosphatase, suggesting that the neoplastic cells were derived from mononuclear cells of the dental follicle, which stain similarly. 4 Studies have shown that mononuclear cells, probably derived from monocytes, 3,4,6 begin to accumulate in the dental follicle of developing teeth immediately prior to eruption. These cells are considered precursors for osteoclasts that appear soon thereafter and are responsible for the resorption of bony tissues and deciduous dental tissues seen during tooth eruption.…”

mentioning

confidence: 99%

“…The cells were positive for vimentin and negative for all other markers. Subsequently, the same blocks were stained histochemically fur tarn-ate-resistant acid phosphatase, a mark- follicular origin for the tumor cells is the expression of tartrate-resistant acid phosphatase, an enzymatic marker of mononuclear cells of the dental follicle 4 with the potential to differentiate into osteoclasts. 1 Multiple neoplasms developed simultaneously at several sites in the mandible and maxilla of this young dog.…”

mentioning

confidence: 99%

Multiple Sarcomas of Dental Follicular Mononuclear Cells in a Five-Month-Old Dog

1994

View full text Add to dashboard Cite

Tartrate-resistant acid phosphatase in bone and cartilage following decalcification and cold-embedding in plastic.

Cited by 181 publications

References 9 publications

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

Multiple Sarcomas of Dental Follicular Mononuclear Cells in a Five-Month-Old Dog

Contact Info

Product

Resources

About