Tartrate-resistant acid phosphatase is not an exclusive marker for mouse osteoclasts in cell culture

Modderman, W. E.; Raap, Achim; Nijweide, Peter J.

doi:10.1016/8756-3282(91)90004-3

Cited by 39 publications

(10 citation statements)

References 21 publications

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“…value of this activity as a reliable marker for osteoclast in culture is discussed [26,27], and they possess receptors for calcitonin [12,28]. Recently, specific antibodies were raised against some osteoclast cell surface antigens, and immunohistochemical methods were proposed for osteoclast identification [29][30][31][32].…”

Section: Discussionmentioning

confidence: 99%

Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone

Basle

Chappard²,

Grizon³

et al. 1993

Calcif Tissue Int

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The nature of the multinucleated cells involved in the resorption processes occurring inside macroporous calcium-phosphate biomaterials grafted into rabbit bone was studied using light microscopy, histomorphometric analysis, enzymatic detection of tartrate-resistant acid phosphatase (TRAP) activity, scanning, and electron microscopy. Samples were taken at days 7, 14, and 21 after implantation. As early as day 7, osteogenesis and resorption were observed at the surface of the biomaterials, inside the macropores. Resorption of both newly formed bone and calcium-phosphate biomaterials was associated with two types of multinucleated cells. Giant multinucleated cells were found only at the surface of the biomaterials; they showed a large number of nuclei, were TRAP negative, developed no ruffled border, and contained numerous vacuoles with large accumulation of mineral crystals from the biomaterials. Osteoclasts exhibited TRAP positivity and well-defined ruffled border. They were observed at the surface of both newly formed bone and biomaterials, around the implant, and inside the macropores. In contract with the biomaterials, infoldings of their ruffled border were observed between the mineral crystals, deeply inside the microporosity. The microporosity of the biomaterials (i.e., the noncrystalline spaces inside the biomaterials) increased underneath this type of cell as compared with underneath giant cells or to the depth of the biomaterials. These observations demonstrate that macroporous calcium-phosphate biomaterials implanted in bone elicit osteogenesis and the recruitment of a double multinucleated cell population having resorbing activity: giant multinucleated cells that resorb biomaterials and osteoclasts that resorb newly formed bone and biomaterials.

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Section: Discussionmentioning

confidence: 99%

Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone

Basle

Chappard²,

Grizon³

et al. 1993

Calcif Tissue Int

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“…There is considerable evidence to suggest that these multinucleated cells originate from hematopoietic stem cells [39][40][41][42][43][44][45][46]. TRAP + activity is generally considered as a marker for osteoclast identification [47] although the specificity of the reaction is still under discussion [48,49]. A ruffled border, composed of a clear zone and a region of cytoplasmic membrane infolding lying against the resorption surface, is a classical ultrastructural element of osteoclasts [38,50].…”

Section: Discussionmentioning

confidence: 99%

Cellular response to calcium phosphate ceramics implanted in rabbit bone

Basle¹,

Rèbel²,

Grizon³

et al. 1993

J Mater Sci: Mater Med

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Two hydroxyapatite ceramics, synthesized by sintering from bovine bone and from a mixture of phosphate tricalcium and natural hydroxyapatite, were implanted in bone sites in rabbits. From day 7 after implantation, osteoblast-like cells were visible with thin layers of new bone on both biomaterials. Histomorphometry showed progressive increase in volume and surface of newly formed bone. Signs of cell-dependent resorption were visible at the surface of biomaterials and newly formed bone. There was a progressive decrease in relative volume and trabecular thickness of the biomaterials. Resorption of biomaterials appears to involve two cell types: multinucleated giant cells and osteoclast-like cells. The multinucleated giant cells observed had neither tartrate resistant acid phosphatase activity (TRAP) nor a ruffled border. Vesicles and vacuoles containing crystals observed in these cells suggest phagocytosis of biomaterials. The number of these cells decreased after day 14 following implantation. The osteoclast-like cells were TRAP positive. The structured modification and the TRAP activity demonstrated in the subjacent biomaterial suggest that the dissolution of the implant may be associated to an extracellular enzymatic activity of these cells. Electron microscopy revealed a clear zone and cytoplasmic membrane infolding in these cells, suggesting a ruffled border differentiation. The number of these cells increased with delay after implantation. It was concluded that the implantation of calcium phosphate ceramics in bone leads to new bone formation as well as to resorption of the biomaterials. The mechanism of resorption appears to associate crystal endocytosis by multinucleated giant cells and more classical resorption by osteoclast-like cells.

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“…However, it is also said to be insufficient to distinguish osteoclasts from other cells with only TRAP activity [8,9] .…”

Section: Introductionmentioning

confidence: 97%

The fluorescent simultaneous azo dye technique for demonstration of tartrate-resistant acid phosphatase (TRAP) activity in osteoclast-like multinucleate cells

1995

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The purpose of our research was to show that the fluorescent simultaneous azo dye technique for tartrate-resistant acid phosphatase (TRAP) activity is useful for demonstration of osteoclasts under a fluorescent microscope and a confocal laser scanning microscope . Osteoclast-like multinucleate cells (MNC) were obtained from cultured mouse calvariae . The fluorescent simultaneous azo dye technique for TRAP activity was applied for cultured MNC . For the observation of specimens, both a conventional fluorescent microscope and a confocal laser scanning microscope for three-dimensional localization of TRAP-positive sites were used . Double staining with the azo dye technique was also adapted for mitochondria and tubrin staining . We found that the fluorescent simultaneous azo dye technique for TRAP activity could be used as a method of demonstrating MNC with a fluorescent microscope. We were also able to obtain the serial localization of three-dimensional sectioning images of TRAP-positive sites in MNC with a confocal laser scanning microscope . Moreover, with double staining of MNC as a modified version of the azo dye technique, we were able to observe both cell localization of TRAP-positive sites and other staining with epifluorescent illumination using the blue excitation method . Therefore, by using the fluorescent azo dye technique for TRAP activity, we can simultaneously observe not only the presence of osteoclasts, but also their characteristic construction and function .

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Tartrate-resistant acid phosphatase is not an exclusive marker for mouse osteoclasts in cell culture

Cited by 39 publications

References 21 publications

Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone

Osteoclastic resorption of Ca-P biomaterials implanted in rabbit bone

Cellular response to calcium phosphate ceramics implanted in rabbit bone

The fluorescent simultaneous azo dye technique for demonstration of tartrate-resistant acid phosphatase (TRAP) activity in osteoclast-like multinucleate cells

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