TAT conjugated cationic noble metal nanoparticles for gene delivery to epidermal stem cells

Peng, Li‐Hua; Niu, Jie; Zhang, Chenzhen; Yu, Wei; Wu, Jiahe; Shan, Ying-Hui; Wang, Xia-Rong; Shen, Youqing; Mao, Zhengwei; Liang, Wenquan; J, Gao

doi:10.1016/j.biomaterials.2014.03.062

Cited by 95 publications

(55 citation statements)

References 51 publications

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“…For gene therapy applications, gold NPs are commonly functionalized with cationic polyamines such as polyethylenimine (PEI) [97] and poly-L-lysine (PLL) [98] that can bind and condense negatively-charged plasmid DNA and confer protection from DNase activity. The surface of gold NPs can also be conjugated with various ligands (e.g., transferrin) [99], antibodies [100, 101], and cell penetrating peptides (e.g., TAT) [97] to facilitate cell entry via receptor-mediated endocytic pathways.…”

Section: Nps For Retinal Gene Therapymentioning

confidence: 99%

“…The surface of gold NPs can also be conjugated with various ligands (e.g., transferrin) [99], antibodies [100, 101], and cell penetrating peptides (e.g., TAT) [97] to facilitate cell entry via receptor-mediated endocytic pathways. Once inside endosomes, PEI, with its high amine density, can function as a “proton sponge” to buffer and trap protons [102].…”

Section: Nps For Retinal Gene Therapymentioning

confidence: 99%

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Nanoparticle-based technologies for retinal gene therapy

Adijanto

Naash

2015

European Journal of Pharmaceutics and Biopharmaceutics

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For patients with hereditary retinal diseases, retinal gene therapy offers significant promise for the prevention of retinal degeneration. While adeno-associated virus (AAV)-based systems remain the most popular gene delivery method due to their high efficiency and successful clinical results, other delivery systems, such as non-viral nanoparticles (NPs) are being developed as additional therapeutic options. NP technologies come in several categories (e.g., polymer, liposomes, peptide compacted DNA), several of which have been tested in mouse models of retinal disease. Here, we discuss the key biochemical features of the different NPs that influence how they are internalized into cells, escape from endosomes, and are delivered into the nucleus. We review the primary mechanism of NP uptake by retinal cells and highlight various NPs that have been successfully used for in vivo gene delivery to the retina and RPE. Finally, we consider the various strategies that can be implemented in the plasmid DNA to generate persistent, high levels of gene expression.

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Section: Nps For Retinal Gene Therapymentioning

confidence: 99%

Section: Nps For Retinal Gene Therapymentioning

confidence: 99%

Nanoparticle-based technologies for retinal gene therapy

Adijanto

Naash

2015

European Journal of Pharmaceutics and Biopharmaceutics

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“…34,35 For example, Koren et al 37 prepared pH-sensitive PEGylated liposomes modified with TAT peptide, which showed increased cytotoxicity with exposed TAT (lower pH values) than shielded TAT (higher pH values). Peng et al 38 synthesized TAT peptide conjugated novel cationic metal nanoparticles as highly efficient carriers for gene delivery to stem cells. Therefore, the TAT peptide was selected to improve gene and drug translocation across the plasma membrane in this project.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the TAT peptide was selected to improve gene and drug translocation across the plasma membrane in this project. Finally, effective intracellular gene and drug release can be achieved by pH-sensitive chemical bonds 38,39 and endosomal escape materials. 40,41 To achieve the pH-responsive release of the chemotherapeutic drug Dox, Dox-PEI (DP) conjugates were prepared through hydrazone bonds based on our group's previous work.…”

Section: Introductionmentioning

confidence: 99%

Multifunctionalized polyethyleneimine-based nanocarriers for gene and chemotherapeutic drug combination therapy through one-step assembly strategy

Jiang¹,

Wang²,

Wang³

et al. 2017

IJN

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Gene therapy combined with chemotherapy to achieve synergistic therapeutic effects has been a hot topic in recent years. In this project, the human tumor necrosis factor-related apoptosis-inducing ligand-encoding plasmid gene ( TRAIL ) and doxorubicin (Dox)-coloaded multi-functional nanocarrier was constructed based on the theory of circulation, accumulation, internalization, and release. Briefly, polyethyleneimine (PEI) was selected as skeleton material to synthesize PEI–polyethylene glycol (PEG)–TAT (PPT). Dox was conjugated to PEI using C6-succinimidyl 6-hydrazinonicotinate acetone hydrazone (C6-SANH), and a pH-sensitive Dox-PEI (DP) conjugate was obtained. Then, intracellular cationic pH-sensitive cellular assistant PPT and DP were mixed to condense TRAIL , and TRAIL –Dox coloaded PPT/DP/ TRAIL (PDT) nanocarriers were obtained by one-step assembly. TRAIL was completely condensed by DP or PPT when mass ratios (DP/PPT to TRAIL ) were up to 100:64, which indicated that DP and PPT could be mixed at any ratio for TRAIL condensation. The intracellular uptake rate of PDT was enhanced ( P <0.05) when the contents of PPT in PPT+DP increased from 0 to 30%. Free Dox and TRAIL -loaded nanocarriers (PPT/C6-SANH-PEI/ TRAIL [PCT]) were selected as controls to verify the synergistic antitumor effects of PDT. Compared with free TRAIL , TRAIL-protein expression was upregulated by PDT and PCT on Western blotting assays. The in vitro cytotoxicity of PDT was significantly enhanced compared to free Dox and PCT ( P <0.01). Furthermore, murine PDT nanocarriers showed higher in vivo antitumor ability than both the Dox group ( P <0.05) and the murine PCT group ( P <0.05). These results indicated that the TRAIL + Dox synergistic antitumor effect could be achieved by PDT, which paves the way to gene–drug combination therapy for cancer.

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“…5 The widest scope of applications are from the biomedical field including exogenous gene/drug delivery systems, advanced biosensors, targeted contrast agents for diagnostic applications and as direct therapeutic agents used in combination with existing treatment modalities. [6][7][8][9][10][11] This diversity of application is especially evident within cancer research, with a myriad of experimental anticancer strategies currently under investigation.…”

mentioning

confidence: 99%

Prostate cancer radiotherapy: potential applications of metal nanoparticles for imaging and therapy

Coulter

Butterworth

Jain

2015

BJR

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Prostate cancer (CaP) is the most commonly diagnosed cancer in males. There have been dramatic technical advances in radiotherapy delivery, enabling higher doses of radiotherapy to primary cancer, involved lymph nodes and oligometastases with acceptable normal tissue toxicity. Despite this, many patients relapse following primary radical therapy, and novel treatment approaches are required. Metal nanoparticles are agents that promise to improve diagnostic imaging and image-guided radiotherapy and to selectively enhance radiotherapy effectiveness in CaP. We summarize current radiotherapy treatment approaches for CaP and consider pre-clinical and clinical evidence for metal nanoparticles in this condition.

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TAT conjugated cationic noble metal nanoparticles for gene delivery to epidermal stem cells

Cited by 95 publications

References 51 publications

Nanoparticle-based technologies for retinal gene therapy

Nanoparticle-based technologies for retinal gene therapy

Multifunctionalized polyethyleneimine-based nanocarriers for gene and chemotherapeutic drug combination therapy through one-step assembly strategy

Prostate cancer radiotherapy: potential applications of metal nanoparticles for imaging and therapy

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