TAT-functionalized PEI-grafting rice bran polysaccharides for safe and efficient gene delivery

Liu, Liang; Yan, Yujian; Ni, Danni; Wu, Shuheng; Chen, Yiran; Chen, Xin; Xiong, Xuemin; Liu, Gang

doi:10.1016/j.ijbiomac.2019.09.234

Cited by 13 publications

(8 citation statements)

References 32 publications

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“…Studies have confirmed that simple incubation of CPPs with selected molecules in the appropriate CR leads to bonding. Literature reports have confirmed the ability of TAT [16], Pep-1 [13], and KALA [17] to form non-covalent bonds. Moreover, each of the selected peptides contains sequences of a cationic character capable of producing electrostatic interactions with negatively charged phosphate residues in plasmid DNA.…”

Section: Discussionmentioning

confidence: 80%

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Low Effectiveness of the Introduction of pmaxGFP into Primary Human Coronary Endothelial Cells Using Cell-Penetrating Peptides and Nuclear-Localization Sequences in Non-Covalent Interactions

et al. 2021

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Cell-penetrating peptides (CPPs), due to their effectiveness and low cytotoxicity, are of increasing interest in the context of the transport of macromolecules to the cells. The simplest and safest method seems to be the non-covalent binding of CPP and cargo molecules. However, it requires the optimization of the reaction conditions. The study aimed to determine the effectiveness and cytotoxicity of the Pep-1, KALA, and TAT proteins as well as the NLS [47–55] and NLS [47–56] sequences derived from the Simian Vacuolating 40 (SV40) T-antigen in the context of the transport of the pmaxGFP plasmid to primary human coronary artery endothelial cells. The results are presented in the form of extensive photographic documentation, which shows significant differences in the efficiency of the transfection process between electroporation and the use of CPPs. The study presents negative results in which, despite the manipulation of various parameters (incubation time, incubation temperature, culture time, charge ratio, plasmid concentration), results similar to electroporation were not obtained.

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Section: Discussionmentioning

confidence: 80%

“…However, regardless of the approach applied, in none of the cases was efficiency even close to that obtained using electroporation by nucleofection ( Figure 22). 9,12,13,16,17,20,21). At the same time, no significant influence of temperature on process efficiency was observed.…”

Section: Resultsmentioning

confidence: 88%

Low Effectiveness of the Introduction of pmaxGFP into Primary Human Coronary Endothelial Cells Using Cell-Penetrating Peptides and Nuclear-Localization Sequences in Non-Covalent Interactions

et al. 2021

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“…Viral vectors have many disadvantages, such as immunogenicity, expensive preparation and low gene carrying capacity, so their application is limited. Non‐viral vectors have several advantages over viral vectors since they are chemically based materials 7,8 . They do not integrate into chromosomes, have low immunogenicity, the ability to deliver large genes and no infective risk, are less expensive and easy to handle, and have the potential for large‐scale production at a reasonable cost 9–12 …”

Section: Introductionmentioning

confidence: 99%

“…Non-viral vectors have several advantages over viral vectors since they are chemically based materials. 7,8 They do not integrate into chromosomes, have low immunogenicity, the ability to deliver large genes and no infective risk, are less expensive and easy to handle, and have the potential for large-scale production at a reasonable cost. [9][10][11][12] Polyethyleneimine (PEI) is considered as a golden standard for gene delivery due to its excellent proton sponge effect and high transfection efficiency.…”

Section: Introductionmentioning

confidence: 99%

Effect of molecular weight of polysaccharide on efficient plasmid DNA delivery by polyethylenimine‐polysaccharide‐Fe(III) complexes

Liu

Yang

Liu

et al. 2022

J of Applied Polymer Sci

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Polyethylenimine (PEI) is considered as the gold standard for evaluating non‐viral gene vectors due to its proton sponge effect and high transfection efficiency. However, its cytotoxicity limits its application. Rice bran polysaccharide (RBP) is rich in nature and beneficial for preparation of biodegradable biomaterials. In this work, three PEI modified RBP‐Fe complexes (PIP‐1, PIP‐2, and PIP‐3) were prepared based on RBP with different molecular weight (Mw). Then the potential of PIP complexes for plasmid DNA (pDNA) delivery and the influence of Mw was studied. PIP complexes showed better safety and higher gene transfection efficiency than PEI. PIP‐1 was prepared by RBP with lower Mw and could formed PIP‐1/pDNA nanoparticle with lower particle size which exhibited higher gene transfection efficiency than other PIP complexes prepared by RBP with higher Mw. The results indicate that the PIP/pDNA complex exhibits a multi‐pathway cellular uptake mechanism. Clathrin dependent and caveolin dependent endocytosis and macropinocytosis were involved in the cellular uptake process of PIP/pDNA complex. It is hoped that this study can provide useful enlightenment for the research and development of gene vector based on polysaccharide.

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“…Once inside the cell, the PEI exhibits its proton sponge effect, buffering, and membrane lytic capacity to benefit the endosomal escape of polyplexes . However, its inherent cytotoxicity caused by its highly positive charge and lack of gene transfer targeting ability limits its clinical application . One strategy to improve PEI is to graft it with central or negatively charged molecules; for example, polysaccharides and PEG have been used to modify PEI to obtain gene carriers with low toxicity and high efficiency due to their good biological compatibility. − However, the amino content in the PEI molecule is decreased due to grafting, thus weakening its DNA condensation ability .…”

Section: Introductionmentioning

confidence: 99%

Effect of Extracellular Matrix Coating on Cancer Cell Membrane-Encapsulated Polyethyleneimine/DNA Complexes for Efficient and Targeted DNA Delivery In Vitro

et al. 2021

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Polyethyleneimine (PEI) has a good spongy proton effect and is an excellent nonviral gene vector, but its high charge density leads to the instability and toxicity of PEI/DNA complexes. Cell membrane (CM) capsules provide a universal and natural solution for this problem. Here, CM-coated PEI/DNA capsules (CPDcs) were prepared through extrusion, and the extracellular matrix was coated on CPDcs (ECM-CPDcs) for improved targeting. The results showed that compared with PEI/DNA complexes, CPDcs had core–shell structures (PEI/DNA complexes were coated by a 6–10 nm layer), lower cytotoxicity, and obvious homologous targeting. The internalization and transfection efficiency of 293T-CM-coated PEI70k/DNA capsules (293T-CP70Dcs) were 91.8 and 74.5%, respectively, which were higher than those of PEI70k/DNA complexes. Then, the internalization and transfection efficiency of 293T-CP70Dcs were further improved by ECM coating, which were 94.7 and 78.9%, respectively. Then, the internalization and transfection efficiency of 293T-CP70Dcs were further improved by ECM coating, which were 94.7 and 78.9%, respectively. Moreover, the homologous targeting of various CPDcs was improved by ECM coating, and other CPDcs also showed similar effects as 293T-CP70Dcs after ECM coating. These findings suggest that tumor-targeted CPDcs may have considerable advantages in gene delivery.

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TAT-functionalized PEI-grafting rice bran polysaccharides for safe and efficient gene delivery

Cited by 13 publications

References 32 publications

Low Effectiveness of the Introduction of pmaxGFP into Primary Human Coronary Endothelial Cells Using Cell-Penetrating Peptides and Nuclear-Localization Sequences in Non-Covalent Interactions

Low Effectiveness of the Introduction of pmaxGFP into Primary Human Coronary Endothelial Cells Using Cell-Penetrating Peptides and Nuclear-Localization Sequences in Non-Covalent Interactions

Effect of molecular weight of polysaccharide on efficient plasmid DNA delivery by polyethylenimine‐polysaccharide‐Fe(III) complexes

Effect of Extracellular Matrix Coating on Cancer Cell Membrane-Encapsulated Polyethyleneimine/DNA Complexes for Efficient and Targeted DNA Delivery In Vitro

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