Taxol as a radiation sensitizer: A flow cytometric study

Effects of paclitaxel in combination with radiation on human head and neck cancer cells (ZMK-1), cervical squamous cell carcinoma (CaSki), and breast adenocarcinoma cells (MCF-7)

“…In vitro the cytotoxic activity of the drug depends on the concentration and the incubation time as well as on the intrinsic sensitivities of the tumor cells tested (Choy et al 1993;Cook et al 1993;Rave-FraÈ nk et al 1997;Steren et al 1993b;Hei et al 1994;Stromberg 1995). With the exception of Liebmann et al (1993), who found a maximal cell killing between 5 nM and 100 nM and no further paclitaxel eect at higher drug concentrations, a cell kill of nearly 100% for paclitaxel-concentrations higher than 100 nM has been described.…”

“…Since 1992, numerous reports on in vitro radiosensitization by paclitaxel have been published involving breast (Liebmann et al 1994b;Steren et al 1993b), brain (Tischler et al 1992a), leukemic (Choy et al 1993), cervical (Geard and Jones 1994), lung (Liebmann et al 1994b), ovarian (Liebmann et al 1994b;Schi et al 1979), prostatic (Steren et al 1993b), colon (Steren et al 1993b), and vulvar (Jaakola et al 1996) cancer cell lines. However, the results remained inconsistent.…”

Effects of Paclitaxel in Combination with Ionizing Radiation on Angiogenesis in the Chick Embryo Chorioallantoic Membrane

“…Paclitaxel, a microtubular inhibitor blocking and/or prolonging cells in G2/M-phase, has been described for its radiosensitizing effects on many cell lines, including the leukemia cell line HL60 [5], the breast cell line MCF-7 and the ovarian cell lines of OVG-1 and BG-1 [22] and has been used in a number of clinical studies with promising results [25,35,39,42,43,48].…”

“…The results from these studies are variable and reveal that endothelial cells differ in their sensitivity to ionizing radiation, probably because of their heterogenicity and different cell kinetics [11]. Although the in vivo models for studying angiogenesis are more reliable, their possible role and utility in radiobiology have not been studied adequately.Paclitaxel, a microtubular inhibitor blocking and/or prolonging cells in G2/M-phase, has been described for its radiosensitizing effects on many cell lines, including the leukemia cell line HL60 [5], the breast cell line MCF-7 and the ovarian cell lines of OVG-1 and BG-1 [22] and has been used in a number of clinical studies with promising results [25,35,39,42,43,48].In the present study, we investigated the effects of a single concentration of paclitaxel on angiogenesis, in combination with various doses of ionizing radiation, using the chick embryo CAM system. …”

Kombinierte Radiochemotherapie des nichtkleinzelligen Bronchialkarzinoms mit Taxol

“…Die Ergebnisse sind allerdings zum Teil widerspruchlich, in manchen (auch Bronchialkarzinom-)Zellinien konnte ein strahlenvetstarkender Effekt trotz G2/M-Block nicht nachgewiesen werden[22]. 1m Gegensatz dazu wurde ein strahlenverstarkender Effekt auch ohne G2/M-Block und in Konzentrationen unterhalb der Zytotoxizitat gezeigt[40]. Auch dem Losungsvermittler Cremophor EL wird ein geringer EinfluB auf den Zellzyklus zugeschrieben[11,23].…”