Effects of Paclitaxel in Combination
with Ionizing Radiation on Angiogenesis in the Chick Embryo
Chorioallantoic Membrane

Kardamakis, Dimitrios; Hadjimichael, Christos; Ginopoulos, P.; Papaioannou, Stamatis

doi:10.1007/s00066-004-1140-6

Cited by 9 publications

(5 citation statements)

References 33 publications

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“…While the effect of a limited number of drugs in combination with IR has been studied on the CAM , we now demonstrate that the efficacy of combining IR with sunitinib is dependent on the schedule that is used. In agreement with others, we show that IR alone reduces angiogenesis and that this recovers within 96 h . Interestingly, our data suggest that the effect of IR on the vasculature is also transient when IR is followed by sunitinib treatment, as this combination treatment schedule was as effective as sunitinib alone.…”

Section: Discussionmentioning

confidence: 74%

Optimal treatment scheduling of ionizing radiation and sunitinib improves the antitumor activity and allows dose reduction

et al. 2015

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The combination of radiotherapy with sunitinib is clinically hampered by rare but severe side effects and varying results with respect to clinical benefit. We studied different scheduling regimes and dose reduction in sunitinib and radiotherapy in preclinical tumor models to improve potential outcome of this combination treatment strategy. The chicken chorioallantoic membrane (CAM) was used as an angiogenesis in vivo model and as a xenograft model with human tumor cells (HT29 colorectal adenocarcinoma, OE19 esophageal adenocarcinoma). Treatment consisted of ionizing radiation (IR) and sunitinib as single therapy or in combination, using different dose-scheduling regimes. Sunitinib potentiated the inhibitory effect of IR (4 Gy) on angiogenesis. In addition, IR (4 Gy) and sunitinib (4 days of 32.5 mg/kg per day) inhibited tumor growth. Ionizing radiation induced tumor cell apoptosis and reduced proliferation, whereas sunitinib decreased tumor angiogenesis and reduced tumor cell proliferation. When IR was applied before sunitinib, this almost completely inhibited tumor growth, whereas concurrent IR was less effective and IR after sunitinib had no additional effect on tumor growth. Moreover, optimal scheduling allowed a 50% dose reduction in sunitinib while maintaining comparable antitumor effects. This study shows that the therapeutic efficacy of combination therapy improves when proper dose-scheduling is applied. More importantly, optimal treatment regimes permit dose reductions in the angiogenesis inhibitor, which will likely reduce the side effects of combination therapy in the clinical setting. Our study provides important leads to optimize combination treatment in the clinical setting.

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Section: Discussionmentioning

confidence: 74%

Optimal treatment scheduling of ionizing radiation and sunitinib improves the antitumor activity and allows dose reduction

et al. 2015

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“…In 2001 Karnabatidis and colleagues evaluated the effect of ionizing radiation on the angiogenesis process in the CAM model [261]. A study on the effects of combining ionizing radiation with drug treatment did not reveal any anti-angiogenic effect of the drug, but further confirmed the notion that the CAM is a convenient model for radiobiological studies [262].…”

Section: Radiation Studies In and Ex Ovomentioning

confidence: 97%

Implementation of the Chick Chorioallantoic Membrane (CAM) Model in Radiation Biology and Experimental Radiation Oncology Research

Dünker

Jendrossek

2019

Cancers

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Radiotherapy (RT) is part of standard cancer treatment. Innovations in treatment planning and increased precision in dose delivery have significantly improved the therapeutic gain of radiotherapy but are reaching their limits due to biologic constraints. Thus, a better understanding of the complex local and systemic responses to RT and of the biological mechanisms causing treatment success or failure is required if we aim to define novel targets for biological therapy optimization. Moreover, optimal treatment schedules and prognostic biomarkers have to be defined for assigning patients to the best treatment option. The complexity of the tumor environment and of the radiation response requires extensive in vivo experiments for the validation of such treatments. So far in vivo investigations have mostly been performed in time- and cost-intensive murine models. Here we propose the implementation of the chick chorioallantoic membrane (CAM) model as a fast, cost-efficient model for semi high-throughput preclinical in vivo screening of the modulation of the radiation effects by molecularly targeted drugs. This review provides a comprehensive overview on the application spectrum, advantages and limitations of the CAM assay and summarizes current knowledge of its applicability for cancer research with special focus on research in radiation biology and experimental radiation oncology.

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“…Combined effects of paclitaxel and single or fractionated doses of ionizing radiation during angiogenesis were also evaluated [22]. X-rays suppression of angiogenesis includes anti-proliferative action and induction of nitric oxide (NO) which also inhibits vascular growth [158].…”

Section: Cam As a Screening Platformmentioning

confidence: 99%

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and / or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

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Effects of Paclitaxel in Combination with Ionizing Radiation on Angiogenesis in the Chick Embryo Chorioallantoic Membrane

Abstract: These data confirm that the CAM system can be conveniently and properly used for radiobiological studies and indicate that paclitaxel in combination with ionizing radiation does not inhibit further angiogenesis in the system used.

Cited by 9 publications

References 33 publications

Optimal treatment scheduling of ionizing radiation and sunitinib improves the antitumor activity and allows dose reduction

Optimal treatment scheduling of ionizing radiation and sunitinib improves the antitumor activity and allows dose reduction

Implementation of the Chick Chorioallantoic Membrane (CAM) Model in Radiation Biology and Experimental Radiation Oncology Research

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

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