Taxol® and baccatin III production in suspension cultures of Taxus baccata and Taxus wallichiana in an airlift bioreactor

Navia-Osorio, Alberto; Garden, H.; Cusidó, Rosa M.; Palazón, Javier; Alfermann, A. W.; Piñol, M. Teresa

doi:10.1078/0176-1617-00576

Cited by 37 publications

(16 citation statements)

References 13 publications

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“…El tamaño de inóculo, periodo de subcultivo, intensidad de luz y fotoperiodo, son factores decisivos para la multiplicación, apariencia fenotípica, producción de taxol y proliferación de células de Taxus spp. (Hirasuna, Pestchanker, Srinivasan, & Shuler, 1996;Navia-Osorio et al, 2002). Además, la suplementación de sustancias orgánicas tales como: caseína hidrolizada, polivinil pirrolidona (PVP), ácido ascórbico y aminoácidos esenciales como glutamina, ácido aspártico, prolina, fenilalanina y vitaminas en el medio, puede incrementar el crecimiento celular de los callos y células de Taxus spp.…”

Section: Producción De Taxoides En Cultivos In Vitrounclassified

“…Además, la suplementación de sustancias orgánicas tales como: caseína hidrolizada, polivinil pirrolidona (PVP), ácido ascórbico y aminoácidos esenciales como glutamina, ácido aspártico, prolina, fenilalanina y vitaminas en el medio, puede incrementar el crecimiento celular de los callos y células de Taxus spp. (Navia-Osorio et al, 2002).…”

Section: Producción De Taxoides En Cultivos In Vitrounclassified

“…The size of inoculum, time of subculturing, light intensity and photoperiod, are decisive factors for multiplication, phenotypic appearance, taxol production and proliferation of Taxus spp. cells (Hirasuna, Pestchanker, Srinivasan, & Shuler, 1996;Navia-Osorio et al, 2002). Furthermore, the supplementation of organic substances such as: casein hydrolyzate, polyvinyl pyrrolidone (PVP), ascorbic acid and essential amino acids such as glutamine acid, aspartic acid, proline, phenylalanine and vitamins in the medium, can increase cell growth of callus and Taxus spp cells.…”

Section: En Biorreactoresmentioning

confidence: 99%

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TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

Barrales-Cureño¹,

Soto-Hernández²

2012

Rev Cha Se Cie For y del Amb

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RESUMENEl árbol del tejo (Taxus spp.) es una gimnosperma dicotiledónea que pertenece a la familia Taxaceae; se distribuye en America, Europa y Asia. Taxus spp. contiene en la corteza y en las hojas, alcaloides diterpénicos conocidos como taxoides. Éstos se caracterizan químicamente por la presencia de un esqueleto de taxano y un anillo de oxetano, tal como el taxol, que es utilizado en el tratamiento del cáncer de ovario, seno, próstata y pulmón; enfermedad que actualmente es una de las principales causas de muerte a nivel mundial. El contenido de taxol es bajo en los árboles, por tanto, es necesario el uso de alternativas biotecnológicas como lo es el cultivo in vitro de tejidos vegetales para incrementar la cantidad de taxoides y preservar el recurso natural. En esta revisión científica se reportan las características químicas de los taxoides, el mecanismo de acción biológico y semisíntesis orgánica del taxol, se detalla también la ruta biosintética de los principales taxoides, así como las principales enzimas involucradas para la obtención de éstos a nivel bioquímico y, finalmente, se indica de manera general la producción de taxoides en cultivos in vitro. ABSTRACTThe yew tree (Taxus spp.) is a dicot gymnosperm belonging to the Taxaceae family and it can be found in America, Europe and Asia. These trees have in their bark and leaves diterpenic alkaloids known as taxoids, which are chemically characterized by the presence of a taxane skeleton and an oxetane ring such as taxol, which is used in the treatment of ovarian, breast, prostate and lung cancer; disease that is currently one of the leading causes of death worldwide. Taxol content is low in trees, so it is necessary to use biotechnology alternatives such as in vitro cultivation of plant tissue to increase the amount of taxoids and preserve the natural resource. The chemical characteristics of taxoids, the mechanism of the biological action and the semi-synthetic organic of taxol were reported in this scientific review; as well as, the biosynthetic pathway of major taxoids and the main enzymes involved in obtaining taxoids at biochemical level and, finally the production of taxoids in vitro cultures is indicated.

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Section: Producción De Taxoides En Cultivos In Vitrounclassified

Section: En Biorreactoresmentioning

confidence: 99%

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TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

Barrales-Cureño¹,

Soto-Hernández²

2012

Rev Cha Se Cie For y del Amb

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“…Cell growth enhanced by fructose treatment and suppressed by glucose [48]. Bioreactors is final and major phase to industrial scale-up and commercial production [50,51]. Cell suspension cultures of T. baccata and T. wallichiana were achieved in a 20-L airlift bioreactor.…”

Section: Explantsmentioning

confidence: 99%

“…Cell suspension cultures of T. baccata and T. wallichiana were achieved in a 20-L airlift bioreactor. Highest total content of Taxol and baccatin III was reached up to 21.04mg/L and 25.67mg/L, respectively by T. wallichiana culture [51]. Cell culture of T. cuspidata (Japanese yew) from callus derived from immature embryos was carried out for Taxol production in a balloon-type bubble bioreactor in 20-l capacity.…”

Section: Explantsmentioning

confidence: 99%

New Approach To Improve Taxol Biosynthetic

Badi¹,

Abdoosi²,

Farzin³

2015

TJS

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Taxol (Generic name for paclitaxel) is a complicated diterpene compound that is initially isolated from yew plants. Taxol is one of the most exciting natural ingredients to treat various cancers, including breast cancer, ovarian carcinoma, melanoma, and lung cancer. Limited number of yew trees, low content of taxol in plant tissue, slow growing and killing the tree to bark harvesting are significant restrictions that have led to the supply and availability of this important substance faced with challenges. Many attempts to complete synthesis, semi-synthesis, finding new source e.g alternative spices, other plant, microorganisms and establishment of the cell suspension cultures have been carried out. Cell culture is an appropriate strategy for stable production by using a small part of the plant as explants. Elicitors such as methyl jasmonate family compounds are powerfull stimulator for production of taxol. Methyl jasmonate increases the amount of taxol by enhancing the expression levels of taxol biosynthesis enzymes such as Geranyl-geranyl pyrophosphate synthase (GGPPs) and taxadienesynthesis (TDS). New approach including genetic and metabolic engineering can be led to increasing the taxol production by the over-expression of genes controlling limiting steps or by suppressing the undesired taxanes by employing antisense technology. The yew-coding genes associated with the production of taxol have been cloned. However, genetic manipulation strategies are not yet complete. This paper reviews the various ways to improve Taxol production.

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Bioreactors: Airlift Reactors

Merchuk

Camacho

2010

Encyclopedia of Industrial Biotechnology

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ALRs are popular in modern bioprocess research and development and, in many cases, they have found industrial uses. The distinctive characteristics of ALRs are conferred by the fluid dynamics of the liquid‐gas or liquid gas‐solid mixtures: holdup, liquid velocity, and mixing in the riser, gas separator, and downcomer. Only a correct understanding of the interconnection of these regions can make possible the scale‐up of a laboratory device, to pilot or industrial size. Several correlations are available in the literature for the prediction of the fluid dynamic characteristics of the reactor and of the mass and heat transfer coefficients, and a selection is presented in this review. Significant progress has been made in the application of modern computational tools to the simulation and design of ALRs. In parallel, innovative and sophisticated methods have been applied to obtain a deeper insight into the mechanisms of fluid motion and the flow patterns in the reactor. However, no single research group has managed to cover all the variables over a wide range. The engineer confronting scale‐up or de novo design of an ALR must analyze the validity of the correlations and computation methods used for the calculations.

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Taxol® and baccatin III production in suspension cultures of Taxus baccata and Taxus wallichiana in an airlift bioreactor

Cited by 37 publications

References 13 publications

TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

TAXOIDES: METABOLITOS SECUNDARIOS DEL ÁRBOL DEL TEJO (Taxus spp.)

New Approach To Improve Taxol Biosynthetic

Bioreactors: Airlift Reactors

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