Taxon Specific Hybridization Probes for Fiber-digesting Bacteria Suggest Novel Gut-associated Fibrobacter

Gene-centric metagenomics of the fiber-adherent bovine rumen microbiome reveals forage specific glycoside hydrolases

“…S4). SSU 16S rRNA gene sequences matching the genus Ruminococcus, a fibrolytic rumen bacterium, were rare in all libraries (e.g., 7 SSU rRNA gene pyrosequences total), which agrees with previous molecular studies of the rumen (4,8,11,13,32,35,36). Additionally, the distribution of EGTs from the Bacteria …”

“…Bacteria predominate the rumen, with a variety of anaerobic protozoa and fungi (1), and the associated occurrence of bacteriophage is well documented (2). The use of small subunit (SSU) rRNA sequence analysis has allowed for a more complete description of the rumen microbiome and these inventories have demonstrated that a large microbial component remains uncultured (3)(4)(5)(6)(7)(8)(9)(10)(11)(12) and that a high proportion of the fibrolytic population has not been thoroughly described (7,8,13,14). The rumen habitat contains a consortium of microbes that harbor the complex lignocellulosic degradation system for the microbial attachment and digestion of plant biomass.…”

Repeated ruminal dosing of Ruminococcus spp. does not result in persistence, but changes in other microbial populations occur that can be measured with quantitative 16S-rRNA-based probes

“…When these same animals were placed on a diet of 70 % rhodes grass and 30 % lucerne (Medicago sativa) the populations rose to approximately 6 % for both Ruminococcus and Fibrobacter. Lin et al (1994), using S-SFsucc-0650-a-A-20, observed that F. succinogenes varied between approximately 0n5 and 6 % of the population depending on the animal and diet consumed. When cellulolytic bacteria are grown together in diculture, cellulose degradation is often below that of the pure culture alone (Dehority & Scott, 1967 ;Dehority, 1973), which is probably the result of competitive and non-competitive interactions between cellulolytic bacteria.…”

Rumen prokaryotic communities of ruminants under different feeding paradigms on the Qinghai-Tibetan Plateau

“…However, the abundance of Fibrobacter was significantly higher in yaks than in Tibetan sheep, and higher in TMR yaks than NG yaks (Table S3). Lin et al [45] demonstrated a greater variability in the relative abundance of Fibrobacter between animals than between gut compartments, and F. succinogenes predominated over F. intestinalis in bovine and goat rumen, while F. intestinalis was detectable but had low abundance. The distribution and abundance of Fibrobacter is more likely related to their habitat (e.g.…”