Taxonomic assessment of species of the genus Octopus from the northeastern Pacific via morphological, molecular and morphometric analyses

Abstract

Species of the genus Octopus from the northeastern Pacific are ecologically and economically important; however, their taxonomy is confusing and has not been comprehensively assessed. In this study, we performed a taxonomic evaluation of these species considering the morphological characteristics of the original descriptions, a molecular analysis of partial COI-gene sequences, and a traditional morphometry analysis of nine body measurements. Several interesting findings were obtained with our results: for instance, we updated the diagnoses of some species by including characters such as the number of lamellae per demibranch and the presence of chromatophores in the visceral sac; we deposited partial COI-gene sequences of species that had not been incorporated into the GenBank repository; and according to the morphometric analysis, we confirmed that the lengths of arms I–IV are relevant to discriminate the species under study. The taxa evaluated were morphologically, molecularly and morphometrically well-delimited; however, features such as funnel organ shape and arm length proportions in regard to dorsal mantle length are either not included in the diagnosis of the genus Octopus or overlap with other genera. Hence, this information, combined with the results obtained from the molecular analysis, supports the generic re-assignation of two of the species evaluated.

A new species of pygmy Paroctopus Naef, 1923 (Cephalopoda: Octopodidae): the smallest southwestern Atlantic octopod, found in sea debris

“…A recent study using molecular analysis of partial COI gene sequences and traditional morphometry data suggested that O. alecto should be considered Paroctopus (Díaz Santana Iturrios et al 2019). Additionally, Magallón-Gayón et al ( 2019) sequenced the complete mitochondrial genome of O. tchi and pointed out that this species does not belong to the genus Octopus, suggesting that it seems closer to the Paroctopus group.…”