Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.

Feurer, Carole; Clermont, Dominique; Bimet, F.; Candréa, A; Jackson, Mary; Glaser, Philippe; Bizet, Chantal; Dauga, Catherine

doi:10.1099/ijs.0.02907-0

Cited by 49 publications

(23 citation statements)

References 49 publications

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“…Furthermore, mRNA of all TLR genes including TLR 2 remained unaffected by exposure to S. e. (Figure 8A). At the protein level, immunocytochemistry and microscopic analysis confirmed that TLR 2 protein was increased 2.37-fold (P ≤ .001) after 24 Figure 8B, left panel). TNF mRNA expression was measured in HEKs cells treated for 2 hours with C. t. or FSL1 (Pam2CGDPKHPKSF), a synthetic lipopeptide and a specific TLR 2/6 agonist.…”

Section: T-induced Expression Of Inflammatory Genes In Heks and Smentioning

confidence: 82%

“…), 21 while other species, such as toxigenic C. diphtheriae, are classified as serious and potentially life-threatening pathogens. 22,23 The species C. tuberculostearicum (C. t.), characterized by C. Feurer et al in 2004 24 is a major component of the bacterial species that colonize a variety of skin environments, including dry, moist, and sebaceous regions. 25 Several studies have associated C. t. with disease states, including inflammatory breast disease, pancreatic panniculitis, chronic rhinosinusitis, and surgical site infections.…”

Section: Introductionmentioning

confidence: 99%

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Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor‐κB inflammatory signaling pathway in human skin cells

Altonsy

Kurwa

Lauzon

et al. 2020

Immunity Inflam & Disease

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Introduction Corynebacterium tuberculostearicum (C. t.) is a ubiquitous bacterium that colonizes human skin. In contrast to other members of the genus Corynebacterium, such as toxigenic Corynebacterium diphtheriae or the opportunistic pathogen Corynebacterium jeikeium, several studies suggest that C. t. may play a role in skin health and disease. However, the mechanisms underlying these effects remain poorly understood. Methods To investigate whether C. t. induces inflammatory pathways in primary human epidermal keratinocytes (HEKs) and human cutaneous squamous carcinoma cells (SCCs), cell culture, reverse transcription‐polymerase chain reaction (PCR), enzyme‐linked immunosorbent assay, immunofluorescence microscopy, Western blot, chromatin immunoprecipitation‐PCR, small interfering RNA knockdown and luciferase reporter expression system were used. Results Herein, we demonstrate that C. t. upregulates the messenger RNA (mRNA) and protein levels of inflammatory mediators in two human skin cell lines, HEKs and SCCs. We further show activation of the canonical nuclear factor‐κB (NF‐κB) pathway in response to C. t. infection, including phosphorylation of the inhibitor of κB (IκB), the nuclear translocation of NF‐κB subunit (NF‐κB‐P65) and the recruitment of NF‐κB‐P65 and RNA polymerase to the NF‐κB response elements at the promoter region of the inflammatory genes. Lastly, the data confirm that C. t.‐induced tumor necrosis factor mRNA expression in HEKs is toll‐like receptor 2 (TLR2) dependent. Conclusion Our results offer a mechanistic model for C. t.‐induced inflammation in human keratinocytes via TLR2 and activation of IκB kinase and downstream signaling through the canonical NF‐κB pathway. Relevance to chronic inflammatory diseases of the skin and cutaneous oncology is discussed.

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Section: T-induced Expression Of Inflammatory Genes In Heks and Smentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor‐κB inflammatory signaling pathway in human skin cells

Altonsy

Kurwa

Lauzon

et al. 2020

Immunity Inflam & Disease

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“…L. clevelandensis was only first described in 2013 (Harrington et al, 2013) and has since been repeatedly associated with abscess formation (Bell et al, 2016;Menezes et al, 2018); however, very recent research suggests it is a common human (nasal) commensal (Escapa et al, 2018). C. tuberculostearicum has traditionally been termed a 'leprosy-derived' Corynebacterium, having been first isolated from a Lepromatous leprosy case (Brown et al, 1984;Feurer et al, 2004); the OTU sequence here was a unique 100% identity match with this (type) strain Medalle X. Recent research, isolating 18 C. tuberculostearicum strains from human clinical specimens (Hini c et al, 2012), demonstrated multiple antimicrobial resistance in most isolates (but importantly, 100% susceptibility to vancomycin) and classified 7 of the 18 isolates as being clinically relevant to surgical site infection [centers for disease control (CDC) criteria (Henriksen et al, 2010)].…”

Section: Real-world Data Testing: International Space Station Data Setmentioning

confidence: 99%

ANCHOR: a 16S rRNA gene amplicon pipeline for microbial analysis of multiple environmental samples

González

Pitre

Brereton

2019

Environmental Microbiology

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SummaryAnalysis of 16S ribosomal RNA (rRNA) gene amplification data for microbial barcoding can be inaccurate across complex environmental samples. A method, ANCHOR, is presented and designed for improved species‐level microbial identification using paired‐end sequences directly, multiple high‐complexity samples and multiple reference databases. A standard operating procedure (SOP) is reported alongside benchmarking against artificial, single sample and replicated mock data sets. The method is then directly tested using a real‐world data set from surface swabs of the International Space Station (ISS). Simple mock community analysis identified 100% of the expected species and 99% of expected gene copy variants (100% identical). A replicated mock community revealed similar or better numbers of expected species than MetaAmp, DADA2, Mothur and QIIME1. Analysis of the ISS microbiome identified 714 putative unique species/strains and differential abundance analysis distinguished significant differences between the Destiny module (U.S. laboratory) and Harmony module (sleeping quarters). Harmony was remarkably dominated by human gastrointestinal tract bacteria, similar to enclosed environments on earth; however, Destiny module bacteria also derived from nonhuman microbiome carriers present on the ISS, the laboratory's research animals. ANCHOR can help substantially improve sequence resolution of 16S rRNA gene amplification data within biologically replicated environmental experiments and integrated multidatabase annotation enhances interpretation of complex, nonreference microbiomes.

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“…Bacteria of the genus Corynebacterium have been recovered extensively from mammals and the environment (Feurer et al, 2004;Hommez et al, 1999). Except for Corynebacterium diphtheriae, the aetiological agent of diphtheria, most species of the genus are regarded as opportunistic pathogens (Paviour et al, 2002).…”

mentioning

confidence: 99%

Corynebacterium pyruviciproducens sp. nov., a pyruvic acid producer

Tong

Liu

Summanen

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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A coryneform strain, 06-1773O T (5WAL 19168 T ), derived from a groin abscess sample was characterized using phenotypic and molecular taxonomic methods. Comparative analyses revealed more than 3 % divergence of the 16S rRNA gene sequence and about 10 % divergence of the partial rpoB gene sequence from the type strain of Corynebacterium glucuronolyticum. The strain could also be differentiated from C. glucuronolyticum by a set of phenotypic properties. A DNA-DNA relatedness study between strain WAL 19168 T and C. glucuronolyticum CCUG 35055 T showed a relatedness value of 13.3 % (13.7 % on repeat analysis). The genotypic and phenotypic data show that the strain merits classification within a novel species of Corynebacterium. We propose the name Corynebacterium pyruviciproducens sp. nov. for the novel species. The type strain is 06-1773O T (5WAL 19168 T 5CCUG 57046 T 5ATCC BAA-1742 T ).Bacteria of the genus Corynebacterium have been recovered extensively from mammals and the environment (Feurer et al., 2004; Hommez et al., 1999). Except for Corynebacterium diphtheriae, the aetiological agent of diphtheria, most species of the genus are regarded as opportunistic pathogens (Paviour et al., 2002). Compared with those bacteria possessing proof of clinical pathogenicity, less emphasis has been put on Corynebacterium. Recently, however, a report demonstrated that Corynebacterium was the most prevalent bacterial genus associated with chronic wound infections in diabetics (possibly skin flora) (Dowd et al., 2008). In our investigation of the bacteriology of acute wound infections (Finegold et al., 2008), a novel aerobic, rod-shaped, Gram-positive corynebacterium was found. It is similar to Corynebacterium glucuronolyticum (Devriese et al., 2000) by phenotypic and genotypic identification. The aim of this polyphasic study was to describe the isolation and characterization of this novel organism.A groin abscess specimen was collected at Olive View-UCLA Medical Center in 2006, and studied in the Wadsworth Anaerobic Laboratory (WAL). One isolate derived from this specimen, WAL 19168 T , was analysed by phenotypic and genotypic characterization methods.Optimal growth conditions were investigated by cultivating strain WAL 19168 T aerobically and anaerobically on trypticase soy agar with 5 % sheep blood (TSA-II; Becton Dickinson Microbiology Systems) at 20, 37 and 42 u C. Lipid requirement was determined by comparison between the culture in simple brain heart infusion (BHI) broth (MP Biomedicals) and in the same medium supplemented with 1 % Tween 80 after 72 h at 37 u C (Riegel et al., 1994). Further confirmation of lipid stimulation of growth was observed by the 'response to serum' test (Coyle & Lipsky, 1990). For cellular fatty acid (CFA) and metabolic end product (ME) (short-chain volatile and non-volatile fatty acids) analyses, the strains were grown in BHI broth supplemented with 1 % glucose and 5 % rabbit serum (MP Biomedicals). CFAs of the strains were detected with a Hewlett Packard 5890 series II gas chromatograph, wh...

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Taxonomic characterization of nine strains isolated from clinical and environmental specimens, and proposal of Corynebacterium tuberculostearicum sp. nov.

Cited by 49 publications

References 49 publications

Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor‐κB inflammatory signaling pathway in human skin cells

Corynebacterium tuberculostearicum, a human skin colonizer, induces the canonical nuclear factor‐κB inflammatory signaling pathway in human skin cells

ANCHOR: a 16S rRNA gene amplicon pipeline for microbial analysis of multiple environmental samples

Corynebacterium pyruviciproducens sp. nov., a pyruvic acid producer

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