Taxonomic evaluation of the polypore Daedaleopsis tricolor based on morphology and molecular data.

Abstract

Daedaleopsis tricolor and D. confragosa were formerly treated either as separate species or as conspecific. D. tricolor may be recognised by the presence of a lamellate hymenophore and mostly dark pileus surface with small network of grains, but its separate status is not supported by its micromorphology. Occurrence of intermediate forms contributes to uncertainty in species delimitation. Although this problem has been known for many years, no study has yet been aimed at a thorough study of both morphological and molecular data. In the present study, we analysed sequences of ITS rDNA, RPB2 and TEF of several typical specimens of D. tricolor and D. confragosa sampled in the Czech Republic in recent years, two specimens of the supposedly closely related D. septentrionalis, and available sequences from GenBank. Our data show that no studied DNA region supports separation of D. tricolor and D. confragosa as distinct species and that D. septentrionalis is supported as a distinct species according to the ITS rDNA and RPB2 genes. We therefore incline to treat D. tricolor as a variety of D. confragosa. Thorough revision of all species hitherto described in Daedaleopsis including Asian species of uncertain identity is recommended.

Molecular taxonomy and phylogenetics of Daedaleopsis confragosa (Bolt.: Fr.) J. Schröt. From wild cherry in Serbia

“…According to published literature data there are several genomic regions that are applied in fungal taxonomy and phylogenetic investigations: ribosomal IGS (Intergenic spacer (ANDERSON and STASOVSKI, 1992;HENRION et al, 1992;MOUKHAMEDOV et al, 1994;EDEL et al, 1995); mitochondrial rDNA (WHITE et al, 1990); ITS and large subunits of rRNA -nLSU rDNA (ZMITROVICH and MALYSHEVA, 2013); ITS rDNA, RPB2, TEF (KOUKOL et al, 2014) and ITS rDNA (MENTRIDA et al, 2015).…”

“…By suggesting throughout the literature that D. tricolor is ecotype of D. confragosa (KO and JUNG, 1999) or a variety of D. confragosa (KOUKOL et al, 2014) most of the research were based on ITS rDNA region that signifies it as the most widely sequenced DNA region in molecular ecology of fungi and has been recommended as the universal fungal barcode sequence that is used for molecular systematics on the species level or even within species (geographic races) (SCHOCH et al, 2012).…”

“…Research work of the group from Czech Republic (KOUKOL et al, 2014) using, besides RPB2 and TEF marker genes, ITS region as one of the choice marker for studies on molecular taxonomy concluded that no studied DNA region supports separation of D. confragosa and D. tricolor as distinct species but the later one as a variety of D. confragosa. Also Austrian group, MENTRIDA et al (2015) state the similar conclusion that D. confragosa and D. tricolor cannot be separated on species level when using ITS data.…”

Cyclohumulanoid Sesquiterpenes from the Culture Broth of the Basidiomycetous Fungus Daedaleopsis tricolor

“…Daedaleopsis tricolor ( D . tricolor ) is one of the common basidiomycetes, wood-rotting fungi found in Europe [ 22 , 23 , 24 , 25 ] and Asia [ 26 , 27 , 28 ]. This fungus is not toxic but generally inedible.…”