Abstract:This study concerns the taxonomic investigation of the Iranian diploid Triticum gene pool using morphology and PCR-SSCP analysis of ITS nrDNA. A co-occurrence of two character states of leaf indumentum and anther length was found to be diagnostically significant among these taxa. Three SSCP profiles were detected among our specimens. Sequencing the representatives of different SSCP patterns detected three new nrITS haplotypes. Based on the results of this study we concluded that the genus Triticum at diploid l… Show more
“…Genetic diversity in the wheat germplasm opens up new scenarios to the discovery of useful genes or alleles and improvement of varieties with desirable traits, which include both farmer-and breed-preferred traits such as yield potential, large seed, high seed weight and tolerance to both biotic and abiotic stresses [21]. Previously, the genetic diversity and population structure of T. uraru populations have been studied using a large number of marker systems, including morphological characters [22], isozymes [23] and molecular markers [20,24,25].…”
Gomarian (2019) Assessment of genetic diversity in Triticumurartu Thumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers,
“…Genetic diversity in the wheat germplasm opens up new scenarios to the discovery of useful genes or alleles and improvement of varieties with desirable traits, which include both farmer-and breed-preferred traits such as yield potential, large seed, high seed weight and tolerance to both biotic and abiotic stresses [21]. Previously, the genetic diversity and population structure of T. uraru populations have been studied using a large number of marker systems, including morphological characters [22], isozymes [23] and molecular markers [20,24,25].…”
Gomarian (2019) Assessment of genetic diversity in Triticumurartu Thumanjan ex Gandilyan accessions using start codon targeted polymorphism (SCoT) and CAAT-box derived polymorphism (CBDP) markers,
“…The diploid Triticum gene pool in Iran comprises Triticum monococcum Linnaeus (1753: 86) subsp. aegilopoides (Link) Thellung (1918: 470) and Triticum urartu (Nasernakhaei et al 2013). They are characterized by two diagnostic characters: anther length and leaf indumentum (Nasernakhaei et al 2013); these two characters are also useful at the generic level.…”
Section: Introductionmentioning
confidence: 99%
“…aegilopoides (Link) Thellung (1918: 470) and Triticum urartu (Nasernakhaei et al 2013). They are characterized by two diagnostic characters: anther length and leaf indumentum (Nasernakhaei et al 2013); these two characters are also useful at the generic level. In order to provide an overall summary of the diploid Triticum taxa a brief taxonomic key (van Slageren 1994) is reproduced below:…”
In this study, 129 individuals belonging to the Iranian wild diploid Triticum gene pool were screened using PCR-SSCP based on Acc1 and Pgk1 loci. Sequencing of the representatives of different SSCP patterns allowed for detection of three haplotypes for each of the loci. The phylogenetic relationships among the Iranian wild diploid Triticum haplotypes and related genera were analyzed using DNA sequences from these two single copy loci. Despite previous reports that recommended the usefulness of nuclear single copy genes for phylogenetic inference, the Bayesian and parsimony analyses carried out in this study showed that these two loci were not applicable tools in the phylogeny reconstruction of diploid Triticum gene pool. Thus, our findings confirm that phylogenetic relationships among recently diverged species are often difficult to resolve due to insufficient phylogenetic signal in available markers and/or conflict among gene trees.
“…1), which was fully consistent with the diagnostic morphological features of T. monococcum ssp. aegilopoides and T. urartu (van Slageren, 1994; Nasernakhaei et al , 2013). The probable K value could not be inferred based on the Ln P ( D ) value because there was no sudden change in it.…”
Section: Resultsmentioning
confidence: 99%
“…1). The taxonomic identifications were made on the basis of the work of van Slageren (1994) and Nasernakhaei et al (2013). Fig.…”
To preliminarily evaluate the genetic diversity of the Iranian diploidTriticumL. gene pool, in this study, a total of 176 individuals belonging toT. monococcumL. ssp.aegilopoides(Link) Thell. andT. urartuThum. ex Gandil. were pre-screened using single-strand conformation polymorphism (SSCP) analysis of theAcc-1andPgk-1loci. A selected set of 76 DNA samples corresponding to the observed SSCP variants were sequenced for both loci and evaluated for nucleotide diversity associated with the taxonomic groups and geographical regions. We found three haplotypes, including one that was new for Iran, at each locus. Population structure and analysis of molecular variation results proved that the collection evaluated could be genetically divided into two distinct groups, which to a great extent was in accordance with the taxonomic classification. A genetic leakage fromT. monococcumssp.aegilopoidesintoT. urartuwas observed during structure analysis, which was inferred on the basis of occasional outcrossing, despite their inbreeding nature. The results revealed that there was no variation within the populations belonging toT. urartu, while a meaningful variation was found between the geographical regions forT. monococcumssp.aegilopoides. The median-joining networks revealed a conflict between morphology and haplotype variation, which was interpreted on the basis of introgressive hybridization, recombination signature and rapid speciation. In conclusion, we suggest that SSCP analysis is a useful tool in regions where thorough sequencing of an enormous number of DNA samples is time consuming and not affordable.
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