Taxonomic rank of <i>Tanacetum boreale</i> Fisch. ex DC. (Asteraceae)

Abstract

This article presents the results of population-genetic analysis in four populations of Tanacetum vulgare (typical tansy) from Southern Siberia on the basis of the Randomly Amplified DNA Fingerprints (RAF). We used RAF to find certain molecular-genetic differences between the typical tansy and the northern tansy. Since the present results did not show any clear differences between the typical tansy and the northern tansy at species level, we suggest that T. vulgare includes two subspecies: the typical T. vulgare subsp. vulgare and T. vulgare subsp. boreale.

Improved Protocols of ITS1-Based Metabarcoding and Their Application in the Analysis of Plant-Containing Products

“…The approximate price of one sample extraction (including prices for disposable plastic and reagents not included in the extraction kits for December 2017) for the “in-house” CTAB method was 1.61$ that for the DiamondDNA Plant kit was 1.67$ and that for the NucleoSpin Plant II mini kit was 7.92$. The DiamondDNA kit has been successfully used in several published studies [ 44 , 45 , 46 , 47 ], but these studies used it for individual plants, not for processed herbal mixes. Our results are congruent with the results of studies that compared NucleoSpin with other extraction methods and agree that this method produces highly pure DNA and could be applied to a wide range of different templates [ 48 , 49 , 50 ].…”