Taxonomic status of the Mediterranean-endemic goby Pomatoschistus adriaticus Miller, 1973 inferred with both morphological and genetic data

Abstract

Knebelsberger & Thiel, Economidichthys trichonis KJ553425 Greece Trichonis drainage Geiger et al. 2014 Economidichthys pygmaeus KJ553482 Greece Louros drainage Geiger et al. 2014 Species GenBank accession numbers Country Locality Referance Knipowitschia byblisia KJ663641 Turkey Dalaman drainage Geiger et al. Knipowitschia mermere KJ553611 Turkey Gediz drainage Geiger et al. Knipowitschia thessela KJ553503 Greece Pinios drainage Geiger et al. Knipowitschia milleri KJ553658 Greece Acheron drainage Geiger et al. Knipowitschia cf. goerneri KJ553444 Greece Trichonis drainage Geiger et al. Knipowitschia caucasica KJ553633 Turkey Simav drainage Geiger et al. Knipowitschia ephesi KJ553609 Turkey Kuecuek drainage Geiger et al. Knipowitschia longecaudata BOLD/EEFF100-06 Russia Borisovka Unpublished data Knipowitschia montenegrina KJ553451 Montenegro Skadar drainage Geiger et al. Knipowitschia panizzae KJ553452 Montenegro Morinjska drainage Geiger et al. Knipowitschia radovici KJ553656 Croatia Neretva drainage Geiger et al. Knipowitschia mrakovcici KJ553514 Croatia Krka drainage Geiger et al.

Morphometric convergence among European sand gobies in freshwater (Gobiiformes: Gobionellidae)

“…Our phylogeny combines the matrix used in Thacker et al (), derived from the earlier studies of Costa et al (), Geiger et al (), Knebelsberger and Thiel (), and Landi et al (), with new data for Pomatoschistus quagga from the work of Öztürk and Engin (). The primary novel result of this combined hypothesis is the recovery of a monophyletic Pomatoschistus , to the exclusion of P. quagga .…”

“…To correct for this bias, we used phylogenetic comparative methods to evaluate shape change and test for convergence in morphology by combining morphometric landmark data with a phylogeny of species. We expanded upon the calibrated phylogeny from Thacker et al () for sand gobies, adding newly published sequence for Pomatoschistus quagga to that mitochondrial COI dataset (Öztürk & Engin, ; GenBank numbers MK302484‐8, all GenBank accession numbers for specimens used in this analysis are given in the Appendix (Table ). We assembled the matrix using Geneious (Biomatters, Ltd.) version 10.2.6 and performed a Bayesian search as originally described: 10 × 10 7 generations using a GTR + I + G substitution model, run with four simultaneous chains, sampling every 1,000 replications and discarding the first 10% of trees as burn‐in.…”

“…We also use pattern‐based tests of convergence to evaluate whether the freshwater species, which do not form a clade, exhibit significant convergence in body form. To provide an evolutionary framework for these tests, we generate a new sand goby phylogeny, adding to the dataset of Thacker et al () with newly generated sequence data for the enigmatic species P. quagga , derived from the study of Öztürk and Engin ().…”

The new distribution records of recently described gobies: Aegean Sea records for Pomatoschistus nanus Engin & Seyhan Öztürk, 2017 and easternmost records of Buenia massutii Kovačić, Ordines & Schliewen, 2017

“…Nucleotide sequences were aligned using ClustalW (Thompson et al , 1994) implemented in MEGA version X (Kumar et al , 2018) and edited with BioEdit (Hall, 1999). Reference sequences belonging to sand gobies and DBSL group were downloaded from GenBank which were mainly provided by Knebelsberger and Thiel (2014), Kovačić et al (2018) and Seyhan Öztürk and Engin (2019) to investigate genetic relationships. Aligned sequences were compared with existing data and submitted to GenBank with accession numbers: OP580161–OP580167.…”