Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales

Sampaio, José Paulo; Gadanho, Mário; Bauer, Robert; Weiß, Michael

doi:10.1007/s11557-006-0044-5

Cited by 63 publications

(61 citation statements)

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“…Mating experiments were performed using Potato-Dextrose (= PDA), Corn Meal (= CMA), Yeast Mold (= YM) and Malt-extract, Yeastextract, Soytone (= MYP) agars (Kurtzman et al 2011;Sampaio et al 2003;Yarrow 1998). Phenotypic characterisation of isolates was carried out according to Yarrow (1998) and Kurtzman et al (2011) using both solid and liquid media.…”

Section: Physiological and Biochemical Characteristicsmentioning

confidence: 99%

Yeast diversity and species recovery rates from beech forest soils

et al. 2016

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Section: Physiological and Biochemical Characteristicsmentioning

confidence: 99%

Yeast diversity and species recovery rates from beech forest soils

et al. 2016

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“…Reconstructing the evolutionary path leading to the present mating systems of basidiomycetes requires obtaining information on this trait for several extant bipolar and tetrapolar members of the three major lineages. This approach, however, has been hindered owing to the scarcity of data for this trait in putative tetrapolar species of the Pucciniomycotina.Leucosporidium scottii is a Pucciniomycotina yeast species with a typical dimorphic life cycle, comprising heterothallic, homothallic (self-fertile), and apparently asexual individuals (Fell and Statzell-Tallman 1982;Sampaio et al 2003;de Garcia et al 2015). This species belongs to the order Leucosporidiales, which is phylogenetically related to both Sporidiobolales (red yeasts) and Microbotryales (anther-smuts) (Sampaio et al 2003;Aime et al 2006).…”

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“…Leucosporidium scottii is a Pucciniomycotina yeast species with a typical dimorphic life cycle, comprising heterothallic, homothallic (self-fertile), and apparently asexual individuals (Fell and Statzell-Tallman 1982;Sampaio et al 2003;de Garcia et al 2015). This species belongs to the order Leucosporidiales, which is phylogenetically related to both Sporidiobolales (red yeasts) and Microbotryales (anther-smuts) (Sampaio et al 2003;Aime et al 2006).…”

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“…This species belongs to the order Leucosporidiales, which is phylogenetically related to both Sporidiobolales (red yeasts) and Microbotryales (anther-smuts) (Sampaio et al 2003;Aime et al 2006). Early studies by Fell and co-workers aimed at characterizing the mating system of this species had already suggested the presence of a multiallelic tetrapolar system, with at least five and three mating specificities, respectively, for the inferred MAT A and MAT B loci (Fell and Statzell-Tallman 1982).…”

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Evolution of Mating Systems in Basidiomycetes and the Genetic Architecture Underlying Mating-Type Determination in the YeastLeucosporidium scottii

et al. 2015

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In most fungi, sexual reproduction is bipolar; that is, two alternate sets of genes at a single mating-type (MAT) locus determine two mating types. However, in the Basidiomycota, a unique (tetrapolar) reproductive system emerged in which sexual identity is governed by two unlinked MAT loci, each of which controls independent mechanisms of self/nonself recognition. Tetrapolar-to-bipolar transitions have occurred on multiple occasions in the Basidiomycota, resulting, for example, from linkage of the two MAT loci into a single inheritable unit. Nevertheless, owing to the scarcity of molecular data regarding tetrapolar systems in the earliest-branching lineage of the Basidiomycota (subphylum Pucciniomycotina), it is presently unclear if the last common ancestor was tetrapolar or bipolar. Here, we address this question, by investigating the mating system of the Pucciniomycotina yeast Leucosporidium scottii. Using whole-genome sequencing and chromoblot analysis, we discovered that sexual reproduction is governed by two physically unlinked gene clusters: a multiallelic homeodomain (HD) locus and a pheromone/receptor (P/R) locus that is biallelic, thereby dismissing the existence of a third P/R allele as proposed earlier. Allele distribution of both MAT genes in natural populations showed that the two loci were in strong linkage disequilibrium, but independent assortment of MAT alleles was observed in the meiotic progeny of a test cross. The sexual cycle produces fertile progeny with similar proportions of the four mating types, but approximately 2/3 of the progeny was found to be nonhaploid. Our study adds to others in reinforcing tetrapolarity as the ancestral state of all basidiomycetes.KEYWORDS sexual reproduction; mating type; mating-type determination; fungi; Basidiomycota S EXUAL reproduction has a pivotal role in the biology of many eukaryotes and is likely a defining evolutionary innovation of this lineage (Dacks and Roger 1999;Goodenough and Heitman 2014). In addition to promoting genetic variation, required for adaptation to fluctuating environments and longterm survival, sexual reproduction in many fungal species, in particular, has a central role in pathogenic development (Bakkeren et al. 2008;Nadal et al. 2008;Heitman 2010;Heitman et al. 2014). It is thus not surprising that recent advances in genome sequencing have revealed that most fungal species have retained the machinery for sexual reproduction and meiosis (Halary et al. 2011;Dyer and O'Gorman 2012;Gioti et al. 2013;Heitman et al. 2014). However, this resilience to keep sexual competence intact comes with an extremely dynamic evolution of sexual behaviors and mating type-determining mechanisms, which may influence important evolutionary and ecological processes, such as adaptation and speciation (Billiard et al. 2011;Heitman et al. 2013;Nieuwenhuis et al. 2013).In the phylum Basidiomycota, sexual reproduction is often dictated by two independent sets of mating-type (MAT)-specific genes that control different stages of the sexual cycle. These...

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“…Both species are very close relatives, differing in only one base pair in the D1D2 region and four base pairs in the ITS region. For this reason, R. creatinivora recently was renamed by Sampaio et al (2003) as Leucosporidiella creatinivora (Golubev) Sampaio.…”

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Utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts

Middelhoven

Doesburg

2006

Antonie van Leeuwenhoek

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A slow growing bacterial population able to utilize hexamethylelenetetramine (urotropine) as sole source of carbon, nitrogen and energy was isolated from soil. From this crude enrichment culture two bacteria were isolated and identified as Brevundimonas diminuta and a Phyllobacterium sp. by sequencing of 16S ribosomal DNA. These bacteria also grew on urotropine but at a lower rate than the enrichment culture. Addition of glucose to the latter resulted in growth of some yeasts that overgrew the bacteria. Assimilation of urotropine as sole nitrogen source is very common among yeasts, 46 out of 60 species tested showed this characteristic.

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Taxonomic studies in the Microbotryomycetidae: Leucosporidium golubevii sp. nov., Leucosporidiella gen. nov. and the new orders Leucosporidiales and Sporidiobolales

Cited by 63 publications

References 33 publications

Yeast diversity and species recovery rates from beech forest soils

Yeast diversity and species recovery rates from beech forest soils

Evolution of Mating Systems in Basidiomycetes and the Genetic Architecture Underlying Mating-Type Determination in the YeastLeucosporidium scottii

Utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts

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