Taxonomical and functional characterization of Bacillus strains isolated from tomato plants and their biocontrol activity against races 1, 2 and 3 of Fusarium oxysporum f. sp. Lycopersici

Rocha, Francine Yuriko Otsuka; Oliveira, Cristiana Maia de; Silva, Paula Renata Alves da; Melo, Leona Henrique Varial de; Carmo, Margarida Goréte Ferreira do; Baldani, José Ivo

doi:10.1016/j.apsoil.2017.07.025

Cited by 35 publications

(19 citation statements)

References 47 publications

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“…Bacillus subtilis is cited as an antagonist to P. brassicae (Einhorn, Bochow, Huber, & Krebs, 1991) and B. cereus , B. megaterium , e. B. toyonensis, isolated from tomato plants in these same areas, are cited as antagonists to Fusarium oxysporum f sp. lycopersici (Rocha et al., 2017). Liu et al.…”

Section: Discussionmentioning

confidence: 99%

Bacterial composition in brassica‐cultivated soils with low and high severity of clubroot

Saraiva

Bhering

Carmo

et al. 2020

Journal of Phytopathology

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The natural bacterial communities of the soil–plant system are relevant in the process of suppressing diseases caused by soilborne plant pathogens. However, little is known regarding the structure of these communities in soils cultivated with brassica vegetables and their relationship with clubroot severity, a disease caused by Plasmodiophora brassicae. In the present study, the composition of bacterial communities in soils cultivated with cauliflower with distinct levels of disease severity was compared. The bacterial composition in soils with lower and higher clubroot severity differs in phylum and genera levels. Soils associated with plants with lower disease severity showed predominance of Kaistobacter, Flavisolibacter, Sphingobacterium, Koribacter, Nitrospira, Bradyrhizobium and Bacillus. Our study reinforces the importance of management strategies that promote improving the physical–chemical characteristics of soil, which are essential to modulate bacterial populations correlated with plant disease suppression.

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Section: Discussionmentioning

confidence: 99%

Bacterial composition in brassica‐cultivated soils with low and high severity of clubroot

Saraiva

Bhering

Carmo

et al. 2020

Journal of Phytopathology

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“…There are several works in the literature that confirm the presence of Bacillus species living inside plants, such as the species B. toyonensis, B. megaterium, B. cereus, B. aryabhattai, B. stratosphericus, and B. aerius that were identified in association with tomato plants in Brazil (Rocha et al, 2017). Karthik et al (2017) The results obtained by Karthik et al (2017); Rocha et al (2017), and Kushwaha et al (2020) reinforce the importance of endophytic Bacillus strains for the biocontrol and plant growth promotion application because they have several functional characteristics such as synthesis of 3-acetic acid, siderophore, hydrolytic enzyme activities and the ability to solubilize inorganic phosphate and zinc.…”

Section: Discussionmentioning

confidence: 99%

“…It has been observed that the Gram staining test varies with bacterial culture age, that is, at the beginning of growth they are Gram-positive but older cultures are variable ( Mo et al, 2020 ). Other authors have also identified Bacillus species with variable stain ( Keita et al, 2013 ; Rocha et al, 2017 ), indicating that it is not possible to use only this test for characterization of bacilli microorganisms, since it can generate false negative results.…”

Section: Discussionmentioning

confidence: 99%

Endophytic Bacillus Bacteria Living in Sugarcane Plant Tissues and Telchin licus licus Larvae (Drury) (Lepidoptera: Castniidae): The Symbiosis That May Open New Paths in the Biological Control

et al. 2021

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Bacteria of the genus Bacillus can colonize endophytically and benefit several crops including the control of some pest orders. In view of the benefits provided by these microorganisms and in order to find out an efficient biotechnological control for the giant borer, our interest in studying the microorganisms in symbiosis with sugarcane and the giant borer has arisen, since there is no efficient chemical or biological control method for this pest. Therefore, endophytic Bacillus strains were isolated from three sugarcane niches (apoplast fluid, central internode cylinder and roots) and also from the giant borer larvae living inside sugarcane varieties grown in the Northeast region of Brazil. The taxonomical characterization (16S rRNA) of 157 Gram-positive isolates showed that 138 strains belonged to the Bacillus genus. The most representative species were phylogenetically closely related to B. megaterium (11.5%) followed by B. safensis (10.8%), B. cereus (8.9%), B. oleronius (8.9%), B. amyloliquefaciens (7.0%), and B. pacificus (6.4%). BOX-PCR analyses showed very distinct band pattern profiles suggesting a great diversity of Bacillus species within the sugarcane niches and the digestive tract, while the B. cereus group remained very closely clustered in the dendrogram. According to XRE biomarker analysis, eleven strains (FORCN005, 007, 008, 011, 012, 014, 067, 076, 092, 093, and 135) correspond to B. thuringiensis species. Additional studies using conserved genes (glp, gmk, pta, and tpi) indicated that most of these strains were phylogenetically closely related to B. thuringiensis and may be considered different subspecies. In conclusion, this study suggests that the culturable Bacillus species are greatly diversified within the plant niches and showed Bacillus species in the digestive tract of the giant borer for the first time. These results open new perspectives to understand the role and functions played by these microorganisms in symbiosis with this pest and also the possibility of developing an efficient biological control method for the giant borer using strains identified as the B. thuringiensis species.

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“…B. cereus had been reported as growth promoter and systemic resistance inducers in Arabidopsis thaliana (Niu et al 2011), growth promoter in red pepper seedlings and gibberellin producer (Joo et al 2004). In addition, B. toyonensis had been reported as bacteria, which could be used to promote growth of Arabidopsis and Datura (Rahmoune et al 2017) and biocontrol of Fusarium oxysporum on tomato (Rocha et al 2017). On the other hands, B. pseudomycoides and B. antrachis used as PGPR or biocontrol agents of plant pathogens did not widely report, yet.…”

Section: Discussionmentioning

confidence: 99%

Characterizations of endophytic Bacillus strains from tomato roots as growth promoter and biocontrol of Ralstonia solanacearum

et al. 2018

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Yanti Y, Warnita, Reflin, Nasution CR. 2018. Characterizations of endophytic Bacillus strains from tomato roots as growth promoter and biocontrol of Ralstonia solanacearum. Biodiversitas 19: 906-911. Bacterial wilt caused by Ralstonia solanacearum is the most damaging vascular pathogens in tomato and many other crops in tropical, subtropical and warm temperate areas of the world limiting its production. Biological agents such as Plant growth Promoting Rhizobacteria (PGPR) is considered as a potential biological control agent for the suppression of plant diseases such as bacterial wilt. Bacillus spp. are one of the most potential genera of PGPR group used for controlling pathogens and promoting plant growth because of their spore-forming ability which increases their adaptation to the environment. The aims of the research were to isolate Endophytic Bacillus isolates, to characterize its ability as plant growth promoter and pathogen controller, and to identify its molecular genetic using 16S rRNA. Bacillus strains were isolated from healthy tomato roots. All Bacillus spp. strains acquired from isolation were then screened directly on plants in completely randomized design experiments with 3 replications. All potential strains were screened and identified using 16S rRNA with 27F and 1492R primers. Results showed that out of 15 obtained isolates, 6 of them showed a good ability to both promote growth and control R. solanacearum. All isolates were identified as B. Pseudomycoides strain NBRC 101232, B. cereus strain CCM 2010, B. toyonensis strain BCT-7112, B. anthracis strain ATCC 14578, B. cereus strain JCM 2152 and B. cereus ATCC 14579.

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Taxonomical and functional characterization of Bacillus strains isolated from tomato plants and their biocontrol activity against races 1, 2 and 3 of Fusarium oxysporum f. sp. Lycopersici

Cited by 35 publications

References 47 publications

Bacterial composition in brassica‐cultivated soils with low and high severity of clubroot

Bacterial composition in brassica‐cultivated soils with low and high severity of clubroot

Endophytic Bacillus Bacteria Living in Sugarcane Plant Tissues and Telchin licus licus Larvae (Drury) (Lepidoptera: Castniidae): The Symbiosis That May Open New Paths in the Biological Control

Characterizations of endophytic Bacillus strains from tomato roots as growth promoter and biocontrol of Ralstonia solanacearum

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