Taxonomy and phylogeny of some Eimeria (Apicomplexa: Eimeriidae) species of rodents as determined by polymerase chain reaction/restriction-fragment-length polymorphism analysis of 18S rDNA

Abstract

The 18S rDNA genes of 10 Eimeria species from rodents (E. albigulae, E. arizonensis, E. falciformis, E. langebarteli, E. nieschulzi, E. onychomysis, E. papillata, E. reedi, E. separata, E. sevilletensis) were polymerase-chain-reaction (PCR)-amplified, digested with 12 restriction endonucleases, and electophoresed in agarose gels. The resulting fragment patterns (riboprints) distinguished all species except E. sevilletensis from E. falciformis, and E. arizonensis from E. albigulae; the sporulated oocysts of the latter two species and of E. onychomysis are often indistinguishable morphologically. When the restriction fragment data were analyzed using distance and parsimony phylogenetic methods a clade was found consistently, which contained E. arizonensis, E. albigulae, E. onychomysis, E. reedi, and E. papillata. This finding and other results of the phylogenetic analyses agreed and supplemented previous phylogenetic work on the Eimeria of rodents. Riboprinting appears to provide useful data for taxonomic and phylogenetic studies on the genus Eimeria and may be especially practical when samples do not contain enough oocysts for other molecular-based methods.

Genetic characterization and phylogenetic analysis of Eimeria arloingi in Iranian native kids

“…However, this approach can be compromised because qualitative and quantitative features of oocyst morphology often overlap among and vary within species of Eimeria. Thus, molecular techniques have recently been proven useful for the identification or classification of these parasites to overcome the limitations of these traditional approaches (Hnida and Duszynski 1999;Matsubayashi et al 2005;Kawahara et al 2010).…”

Differential miRNA expression in the mouse jejunum during garlic treatment of Eimeria papillata infections

“…papillata infections E. papillata, kindly provided by Prof. Mehlhorn (University of Duesseldorf, Germany), was previously characterized (Danforth et al 1992;Chobotar et al 1993;Hnida and Duszynski 1999;Zhao and Duszynski 2001). Oral gavage of mice was done with 10 3 sporulated oocysts of E. papillata suspended in 100 μl sterile saline.…”

Reporter gene expression in cell culture stages and oocysts of Eimeria nieschulzi (Coccidia, Apicomplexa)

“…The resulting plasmid was named pmic1YFP mut YFP-Tgdhfr. Further modification were accomplished by replacement of the tgdhfr3′UTR with the E. tenella ACT1 3′UTR (#11 and #12) and MIC1 3′UTR (#9 and #10) amplified by PCR from genomic DNA which were prepared according to Zhao et al (2001) and Hnida and Duszynski (1999) (see Fig. 1, Table 1 Primer #1-#16).…”