TC1 (C8orf4) is upregulated by cellular stress and mediates heat shock response

Park, Juhee; Jung, Yoojin; Kim, Jung‐Tae; Kim, Ka-Young; Ahn, Sang‐Gun; Song, Kyuyoung; Lee, Inchul

doi:10.1016/j.bbrc.2007.06.077

Cited by 15 publications

(18 citation statements)

References 26 publications

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“…C, THP-1 adhesion assay after 10 ng/ml IL-1␤ treatment for 6 h in TC1 knocked-down and control HAEC. D, 14 C-sucrose permeability test. Four ϫ 10 4 cells/well HUVEC are seeded on a Transwell filter (Corning), and incubated until a complete monolayer is formed changing the medium every 2 days.…”

Section: Tc1 Expression Is Nf-b-dependentmentioning

confidence: 99%

“…TC1 effect on the permeability of endothelial monolayer was analyzed using the 14 C-sucrose permeability test. HUVEC monolayer was transfected using low-dose DNA, 50 ng/ml.…”

Section: Tc1 Enhances Monocyte-endothelial Adhesion and Endothelial Pmentioning

confidence: 99%

“…Real-time PCR was done as described previously (12,14). Total RNA was extracted using TRIzol reagent (Invitrogen), and cDNA was synthesized using Superscript II reverse transcriptase (Invitrogen).…”

Section: Real-time and Semiquantitative Rt-pcrmentioning

confidence: 99%

“…Interestingly, TC1 is up-regulated by IL-1␤ and TNF-␣, and enhances the proliferation of follicular dendritic cells (12), which are essential for B cell development (13). TC1 is also up-regulated by heat shock and cellular stresses in various cells (14). Together, TC1 appears to be implicated in diverse biological regulations with a potential role in the inflammatory and immune regulation in response to various stimuli.…”

mentioning

confidence: 99%

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TC1(C8orf4) Is a Novel Endothelial Inflammatory Regulator Enhancing NF-κB Activity

Kim¹,

Kim²,

Kim

et al. 2009

The Journal of Immunology

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Endothelial inflammation is regulated by a complex molecular mechanism. TC1(C8orf4) is a novel regulator implicated in cancer and inflammation. It is a small protein conserved well among vertebrates. In zebrafish embryos, it is mostly expressed in angio-hematopoietic system and the overexpression induces edema. In human aortic endothelial cells and umbilical vein endothelial cells, TC1 transfection up-regulates key inflammatory cytokines, enzymes, and adhesion proteins including IL-6, IL-1α, COX-2, CXCL1, CCL5, CCL2, IL-8, ICAM1, VCAM1, and E-selectin, while TC1 knockdown down-regulates them. TC1 also enhances inflammatory parameters such as monocyte-endothelial adhesion and endothelial monolayer permeability. TC1 is up-regulated by IL-1β, TNF-α, LPS, and phorbol ester, and the up-regulation is inhibited by I-κB-kinase inhibitors. TC1, in turn, enhances the nuclear translocation of RelA and the DNA binding activity, suggesting a biological role of amplifying NF-κB signaling via a positive feedback. Our findings suggest that TC1 is a novel endothelial inflammatory regulator that might be implicated in inflammatory vascular diseases.

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Section: Tc1 Expression Is Nf-b-dependentmentioning

confidence: 99%

“…TC1 effect on the permeability of endothelial monolayer was analyzed using the 14 C-sucrose permeability test. HUVEC monolayer was transfected using low-dose DNA, 50 ng/ml.…”

Section: Tc1 Enhances Monocyte-endothelial Adhesion and Endothelial Pmentioning

confidence: 99%

Section: Real-time and Semiquantitative Rt-pcrmentioning

confidence: 99%

mentioning

confidence: 99%

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TC1(C8orf4) Is a Novel Endothelial Inflammatory Regulator Enhancing NF-κB Activity

Kim¹,

Kim²,

Kim

et al. 2009

The Journal of Immunology

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“…The expression is enhanced by pro-inflammatory cytokines, heat shock, and various cellular stresses [18], [19]. It enhances Wnt/β-catenin signaling in cancer cells and is associated with poor prognosis [12], [17].…”

Section: Introductionmentioning

confidence: 99%

TC1(C8orf4) Regulates Hematopoietic Stem/Progenitor Cells and Hematopoiesis

et al. 2014

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Hematopoiesis is a complex process requiring multiple regulators for hematopoietic stem/progenitor cells (HSPC) and differentiation to multi-lineage blood cells. TC1(C8orf4) is implicated in cancers, hematological malignancies and inflammatory activation. Here, we report that Tc1 regulates hematopoiesis in mice. Myeloid and lymphoid cells are increased markedly in peripheral blood of Tc1–deleted mice compared to wild type controls. Red blood cells are small-sized but increased in number. The bone marrow of Tc1 −/− mice is normocellular histologically. However, Lin−Sca-1+c-Kit+ (LSK) cells are expanded in Tc1 −/− mice compared to wild type controls. The expanded population mostly consists of CD150−CD48+ cells, suggesting the expansion of lineage-restricted hematopoietic progenitor cells. Colony forming units (CFU) are increased in Tc1 −/− mice bone marrow cells compared to controls. In wild type mice bone marrow, Tc1 is expressed in a limited population of HSPC but not in differentiated cells. Major myeloid transcriptional regulators such as Pu.1 and Cebpα are not up-regulated in Tc1 −/− mice bone marrow. Our findings indicate that TC1 is a novel hematopoietic regulator. The mechanisms of TC1-dependent HSPC regulation and lineage determination are unknown.

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TC-1 (C8orf4) expression is correlated with differentiation in ovarian carcinomas and might distinguish metastatic ovarian from metastatic colorectal carcinomas

Liu

et al. 2013

Virchows Arch

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Thyroid cancer 1 (TC-1, C8orf4) is involved in the development of many cancers. In this study, we investigated the correlation between the expression of TC-1 and the clinicopathological characteristics of ovarian and colorectal adenocarcinomas. We also explored the possible use of TC-1 as a marker to distinguish between metastatic tumors of the ovary and colorectum. We used immunohistochemistry to examine the expression level of TC-1 in 100 ovarian and 100 colorectal adenocarcinomas and 25 metastatic carcinomas with the ovary or colorectum as primary site. TC-1 was expressed in all ovarian carcinoma samples. The high expression rate of TC-1 was 84 % in ovarian carcinomas, which was much higher than that observed in colorectal adenocarcinomas (35 %, P < 0.001). High expression of TC-1 significantly correlated with poor differentiation of ovarian carcinomas (P = 0.013). To explore the value of TC-1 in distinguishing metastatic ovarian cancers from colorectal cancers, we found the area under the receiver operator characteristic curve of TC-1 to be 0.819 (95 % confidence interval, 0.760-0.878; P < 0.001). Furthermore, TC-1 was highly expressed in 100 % of nine metastatic ovarian cancers, but only in 31 % of 16 metastatic colorectal cancers. The higher expression of TC-1 in ovarian compared to colorectal adenocarcinomas suggests its potential use as a marker, to distinguish between metastatic ovarian and colorectal adenocarcinomas.

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TC1 (C8orf4) is upregulated by cellular stress and mediates heat shock response

Cited by 15 publications

References 26 publications

TC1(C8orf4) Is a Novel Endothelial Inflammatory Regulator Enhancing NF-κB Activity

TC1(C8orf4) Is a Novel Endothelial Inflammatory Regulator Enhancing NF-κB Activity

TC1(C8orf4) Regulates Hematopoietic Stem/Progenitor Cells and Hematopoiesis

TC-1 (C8orf4) expression is correlated with differentiation in ovarian carcinomas and might distinguish metastatic ovarian from metastatic colorectal carcinomas

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