TC21 mediates transformation and cell survival via activation of phosphatidylinositol 3-kinase/Akt and NF-κB signaling pathway

Melanoma cell migration to type IV collagen requires activation of NF-κB

“…Furthermore, dominant negative Rac or Ras expression results in a modest reduction of CIV-induced NF-kB binding activity. PI-3K has also been shown to play a role in inducing NF-kB, by regulating Akt/PKB activity (Burow et al, 2000;Rong et al, 2002), and our preliminary data suggest that PI-3K influences NF-kB activity as well as cell migration (data not shown). Therefore, NF-kB is a likely target of integrin-mediated signaling pathways.…”

Apoptosis signaling by the novel compound 3-Cl-AHPC involves increased EGFR proteolysis and accompanying decreased phosphatidylinositol 3-kinase and AKT kinase activities

“…We have found that overexpression of a constitutively active myristylated AKT results in resistance to 3-Cl-AHPC apoptosis. In addition, enhanced activation of PI3-K and AKT in NIH3T3 cells expressing the mutated Ras family member TC21 Dup 24À26 resulted in resistance to 3-Cl-AHPC-mediated apoptosis and 3-Cl-AHPC-mediated inhibition of AKT phosphoserine 473 levels (Rong et al, 2002 and data not shown). AKT enhances cell survival through the phosphorylation of a number of substrates.…”

Tumor suppressor RASSF1A is a microtubule-binding protein that stabilizes microtubules and induces G2/M arrest

“…These antibodies were subsequently used to detect the endogenous RASSF1A's expression by Western blot analysis as well as its interaction with microtubules by co-immunoprecipitation. Western blot analyses were performed as previously described (Rong et al, 2002). To detect the endogenously expressed RASSF1A protein, cells grown in 100-mm plates were lysed in a lysis buffer as previously described (Rong et al, 2002) and subjected to Western blot analysis.…”

“…Western blot analyses were performed as previously described (Rong et al, 2002). To detect the endogenously expressed RASSF1A protein, cells grown in 100-mm plates were lysed in a lysis buffer as previously described (Rong et al, 2002) and subjected to Western blot analysis. To detect the expression of Myc-tagged or GFP-tagged RASSF1A protein, the control pCEP4 vector and pCEP4 Myc-tagged RASSF1A expression vector or the GFP-tagged RASSF1A and the GFP alone control vector were used in transfections.…”