TcaB, an insecticidal protein from Photorhabdus akhurstii causes cytotoxicity in the greater wax moth, Galleria mellonella

Mathur, Chetna; Phani, Victor; Kushwah, Jyoti; Somvanshi, Vishal Singh; Rao, Uma

doi:10.1016/j.pestbp.2019.03.019

Cited by 11 publications

(10 citation statements)

References 58 publications

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“…Overall, it is assumed that on Txp40 injection, test insects launch an intensive PO‐mediated immune reaction that leads to unrestricted production of quinones, which eventually confer toxicity. A similar result was obtained in G. mellonella injected with Xenorhabdus GroEL toxin, Photorhabdus TcaB toxin and okadaic acid …”

Section: Discussionsupporting

confidence: 83%

“…Hemocyte‐mediated phagocytosis, encapsulation and nodulation of invading alien species are well known cellular immune mechanism of insects . Toxin proteins produced by different bacterial species have been reported to target hemocytes and by negatively regulating hemocyte viability towards induction of apoptosis and cytolysis ultimately compromised insect immunity …”

Section: Discussionmentioning

confidence: 99%

“…Protein concentration was determined via Bradford's assay using bovine serum albumin (BSA) as the reference. The identity of the protein was ascertained by SDS‐PAGE followed by mass spectroscopy of in‐gel tryptic digests as depicted earlier …”

Section: Methodsmentioning

confidence: 99%

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Txp40, a protein from Photorhabdus akhurstii, conferred potent insecticidal activity against the larvae of Helicoverpa armigera, Spodoptera litura and S. exigua

Shankhu

Mathur

Mandal

et al. 2020

Pest Management Science

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BACKGROUND: Txp40, a 37 kDa protein, previously characterized from the Gram-negative bacterium Photorhabdus akhurstii (symbiotically associates with insect-parasitic nematode, Heterorhabditis indica), conferred insecticidal activity against Galleria mellonella. Here, the biological activity of Txp40 was evaluated against economically important insects, including Helicoverpa armigera, Spodoptera litura and S. exigua.RESULTS: When both intra-hemocoel injected and orally fed to test insects, comparatively greater oral LD 50 (187.7-522 ng g −1 ) than injection LD 50 (32.33-150.6 ng g −1 ) was obtained with Txp40 derived from P. akhurstii strain IARI-SGMG3

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

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Txp40, a protein from Photorhabdus akhurstii, conferred potent insecticidal activity against the larvae of Helicoverpa armigera, Spodoptera litura and S. exigua

Shankhu

Mathur

Mandal

et al. 2020

Pest Management Science

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“…A stock culture of E. coli strain BL21 [DE3) containing the recombinant pET29a:: TcaB expression clone was maintained in our laboratory. The methodology for TcaB cloning, expression, and purification are detailed in 21 and 22 . Briefly, the recombinant E. coli cells were cultured in LB medium containing kanamycin (50 µg mL −1 ) at 37°C for 4 h or until the absorbance reached 0.6 at 600 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Next, a cytotoxic effect on hemocytes was documented, which was similar to apoptotic cell death. In parallel, TcaB caused an immunomodulatory effect by elevating the hemolymph phenoloxidase activity [ 21 , 22 ]. We identified a catalytic activity domain and a receptor binding domain in the TcaB sequence.…”

Section: Introductionmentioning

confidence: 99%

The induced knockdown of GmCAD receptor protein encoding gene in Galleria mellonella decreased the insect susceptibility to a Photorhabdus akhurstii oral toxin

et al. 2021

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Photorhabdus bacteria secrete a repertoire of protein toxins that can kill the host insect. Among them, toxin complex (Tc) proteins have gained significant attention due to their wider conservation across the different bacterial genera. In our laboratory, a C-terminal domain of TcaB protein was characterized from P. akhurstii bacterium that conferred the potent oral insecticidal effect on Galleria mellonella . However, the role of insect gut receptors in the TcaB intoxication process was yet to be investigated. In the current study, we examined the transcription of candidate midgut receptors in TcaB-infected larvae and subsequently cloned a cadherin-like gene, GmCAD , from G. mellonella. GmCAD was highly transcribed in the fourth-instar larval stage and specifically in the midgut tissues. Our ligand blot and binding ELISA assays indicated that TcaB binds to the truncated peptides from the GmCAD transmembrane-proximal region with greater affinity than that from the transmembrane-distal region. Oral administration of bacterially expressed GmCAD dsRNA in G. mellonella severely attenuated the expression of target mRNA, which in turn alleviated the negative effect of TcaB on insect survival (TcaB-induced mortality in CAD dsRNA pretreated larvae reduced by 72–83% compared to control), implying the association of GmCAD in the TcaB intoxication process. Present findings form a basis of future research related to the insect gut receptor interactions with Photorhabdus toxins.

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A Photorhabdus akhurstii toxin altered gut homeostasis prior conferring cytotoxicity in Spodoptera frugiperda, S. litura and Helicoverpa armigera

et al. 2021

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TcaB, an insecticidal protein from Photorhabdus akhurstii causes cytotoxicity in the greater wax moth, Galleria mellonella

Cited by 11 publications

References 58 publications

Txp40, a protein from Photorhabdus akhurstii, conferred potent insecticidal activity against the larvae of Helicoverpa armigera, Spodoptera litura and S. exigua

Txp40, a protein from Photorhabdus akhurstii, conferred potent insecticidal activity against the larvae of Helicoverpa armigera, Spodoptera litura and S. exigua

The induced knockdown of GmCAD receptor protein encoding gene in Galleria mellonella decreased the insect susceptibility to a Photorhabdus akhurstii oral toxin

A Photorhabdus akhurstii toxin altered gut homeostasis prior conferring cytotoxicity in Spodoptera frugiperda, S. litura and Helicoverpa armigera

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