Tcf-1 gene silence suppresses downstream gene expression in CD4+ T cells from bone marrow of aplastic anemia patients

Abstract

CD4(+) T cells play a crucial role in the pathogenesis of acquired aplastic anemia (AA). Tcf-1 gene regulates T cell development and function, and it is significantly upregulated in the bone marrow CD4(+) T cells from patients with acquired AA. To explore the role of Tcf-1 in the pathogenesis of AA, we knocked down Tcf-1 gene in CD4(+) T cells of AA patients and studied the effects of Tcf-1 silencing on its downstream gene expression. Upon transfection of psiRNA into marrow CD4(+) T cells from bone marrow of aplastic anemia patients, the expression of Tcf-1 was significantly knocked down; consequently, expressions of c-Myc and CD44 were also significantly reduced. Our results suggest that Tcf-1 may contribute to pathogenesis of AA by regulating downstream gene expression such as c-myc and CD44.

Intraflagellar Protein 88 Interactome Analysis: A Bioinformatics Approach Highlights Its Role in Testis and Sperm Function

“…A second subnetwork is identified in IFT88 interactome. The proteins that compose this subnetwork are involved, according to the literature, in cancer, renal disease and diabetes [34]- [42]. The interconnectivity between this proteins and the fact that IFT88 interacts with all of them may indicate the involvement of IFT88 in a common pathophysiological mechanism.…”