MaterialsSpectrophotometer (Thermo Scientific), oven (Memmert), rotary evaporator (Heidolph), soxhlet (Iwaki), desiccator (Iwaki), vortex (Heidoplh), analytical balance (Ohaus), Hot plate (Thermo Scientific), Micropipette (Socorex). Ethanol 70% (Smart Lab), ethanol 96% (Merck), aluminum chloride (Merck), quercetin (Merck), gallic acid (Merck), Folin-Ciocalteu reagent (Merck), ascorbic acid (Merck), sodium acetate (Merck), DPPH radical (Merck), sodium bicarbonate (Merck).
Plant determinationP. americana seeds were determined to derive from the Persea americana Mill (No. B-785/V/ DI.05.07/3/2022) plant species at the Badan Riset Dan Inovasi Nasional (BRIN) Cibinong, Indonesia.
ExtractionThe 70% ethanol extract from P. americana seeds was prepared using two methods, maceration (cold methods), 8 dan reflux (hot methods). 16 The maceration method is made by weighing 100 g of