Tea Flavonoids Induced Differentiation of Peripheral Blood-derived Mononuclear Cells into Peripheral Blood-derived Endothelial Progenitor Cells and Suppressed Intracellular Reactive Oxygen Species Level of Peripheral Blood-derived Endothelial Progenitor Cells

Widowati, Wahyu; Wijaya, Laura; Laksmitawati, Dian Ratih; Widyanto, Rahma Micho; Erawijantari, Pande Putu; Fauziah, Nurul; Bachtiar, Indra; Sandra, Ferry

doi:10.20307/nps.2016.22.2.87

Cited by 6 publications

(17 citation statements)

References 20 publications

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“…32 Later, it was found that EGCG, ECG, EGC and C of tea extract could induce differentiation of peripheral blood mononuclear cells (PB-MNCs) into peripheral blood-derived EPCs (PBEPCs). 31 The resulted PB-EPCs were confirmed by flow cytometry to detect EPCs membrane markers expressions (CD34, CD133, VEGFR-2). Treatment of EGCG, ECG, EGC and C increased the percentages of all CD34, CD133, VEGFR-2 expressions.…”

Section: Tea Extractmentioning

confidence: 96%

“…Herbal extracts were now highly investigated due to its potential to induce cell proliferation. [31][32][33][34] The following are some potential herbal extracts in inducing stem cell proliferation.…”

Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning

confidence: 99%

“…Tea extract consists of epigallocatechin gallate (EGCG), epicatechin gallate (ECG), epigallocatechin (EGC) and catechin (C). 31 EGCG was reported to induce proliferation of adipose tissue-derived mesenchymal stem cells (AD-MSCs). In addition, EGCG induces differentiation of AD-MSCs into endothelial progenitor cells (EPCs) as well.…”

Section: Tea Extractmentioning

confidence: 99%

“…Treatment of EGCG, ECG, EGC and C increased the percentages of all CD34, CD133, VEGFR-2 expressions. 31 In addition, EGCG, ECG, EGC and C could protect PB-EPCs from oxidative damage by suppressing the intracellular ROS levels as well. 31 Therefore tea flavonoids might be related to PB-EPCs production and protection.…”

Section: Tea Extractmentioning

confidence: 99%

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Role of Herbal Extract in Stem Cell Development

Sandra

2018

Mol Cell Biomed Sci

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Stem cell research has been developed and today we can witness some stem cell clinical trials are on going in Indonesia. To meet a successful stem cell treatment, several factors need to be considered, such as cell number. Cell number has been reported to be crucial, and therefore optimal cell number should be achieved. Meanwhile, in some circumstances, cell number is not enough, therefore, cell number should be enriched in an in vitro stem cell culture setting. In an in vitro stem cell culture, besides suitable and sterile equipment, reagents such as culture medium, serum and antibiotics are all important. Although all those criteria are fulfilled, somehow stem cell enrichment cannot be achieved, cell number is still below the target. Modification of stem cell microenvironment should then be an alternative. The addition of growth factors is a part of the strategies to reach a better enrichment. So that, stem cells could later be induced to proliferate at a higher rate. This strategy was then pursued by the scientist involved in herbal medicine. Herbal extracts were now highly investigated due to its potential to induce cell proliferation. Some herbal extracts inducing proliferation and differentiation of stem cell will be shown and described.Keywords: herbal extract, stem cell, progenitor cell, proliferation, differentiation

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Section: Tea Extractmentioning

confidence: 96%

Section: B I O P H a R M A C E U T I C A L I N S T I T U T Ementioning

confidence: 99%

Section: Tea Extractmentioning

confidence: 99%

Section: Tea Extractmentioning

confidence: 99%

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Role of Herbal Extract in Stem Cell Development

Sandra

2018

Mol Cell Biomed Sci

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“…Previous studies showed that several antioxidants could improve EPCs growth and stem cells differentiation into EPCs [10,11]. Impaired proliferation of EPCs in cardiovascular-related disease was also able to be enhanced with antioxidant treatment [12,13].…”

Section: Introductionmentioning

confidence: 98%

Preliminary Study: Purple Sweet Potato Extract Seems to Be Superior to Increase the Migration of Impaired Endothelial Progenitor Cells Compared to l-Ascorbic Acid

et al. 2019

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Impairment of the endothelial progenitor cells (EPCs) ability to proliferate and migrate in the patients with coronary heart disease (CHD) is partly caused by oxidative stress. This research evaluates the effect of treatment with Ipomoea batatas L./purple sweet potato (PSP) extract and l-ascorbic acid on the proliferation and migration of impaired EPCs. EPCs were isolated from CHD patient’s peripheral blood. EPCs culture were cultivated and divided into control (untreated), PSP extract treatment (dose 1 and 25 μg/mL), and l-ascorbic acid treatment (dose 10 and 250 μg/mL) groups for 48 h. EPCs proliferation was analyzed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell proliferation assay, and migration was evaluated with the cell migration assay kit. Statistical tests were evaluated using SPSS 25.0. This research showed that EPCs proliferation and migration was significantly higher in all PSP extract and l-ascorbic acid treatment compared to the control (p < 0.001). EPCs migration on treatment with a PSP extract dose of 25 μg/mL was significantly higher compared to the treatment with l-ascorbic acid dose of 250 μg/mL (303,000 ± 1000 compared to 215,000 ± 3000 cells, p< 0.001). In conclusion, both treatments with PSP extract and l-ascorbic acid can improve the proliferation and migration of impaired EPCs. At the dose of 25 μg/mL, PSP extract seems to be superior to the l-ascorbic acid dose of 250 μg/mL to improve EPCs migration.

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