The relevance of the study is due to the current lack of data on cortical mechanisms of protein metabolism regulation in dogs, which is important to consider when developing methods of corrective action on metabolism. The aim of the study was to determine the peculiarities of protein metabolism in dogs with different types of higher nervous activity, and to establish the relationship between the main characteristics of nervous processes and indicators of protein metabolism in the blood. The leading method in the study of this issue was a new method for determining the typological features of the nervous system in dogs, and the obtained scores of strength, balance, and mobility of nervous processes helped to identify the degree of their influence on protein metabolism. A significant effect of short-term nutritional deprivation on the content of certain indicators of protein metabolism in the blood of dogs, depending on their temperament, was established. Under the action of the stimulus during the day, a decrease in the level of albumin and changes in the ratio of globulins were observed in the blood of dogs, in particular, an increase in the relative content of α- and β-globulins and a decrease in γ-globulins. It was determined that the type of higher nervous activity has a significant effect on the content of total protein, albumin, globulins, β-globulins, γ-globulins and the value of the albumin-globulin ratio (F=3.02-14.6>FU=2.90; P<0.05-0.001) in the blood plasma of dogs. A direct relationship between the strength of nervous processes and the content of total protein and albumin, γ-globulins (r=0.62-0.73; P<0.01) and an inverse relationship between the content of αand β-globulins (r=-0.51-0.56; P<0.01) was found three days after the onset of short-term nutritional deprivation. The balance of nervous processes was inversely related to the content of β-globulins (r=-0.44; P<0.05). The fundamental knowledge gained is of practical value for the development of new, modern methods of metabolic correction taking into account the temperament of animals