Background: Active learning methods are strategies used by most universities worldwide to enhance the problem-solving ability and develop critical thinking in students. Team-based learning (TBL) is one of these methods, in which students study the lesson prior to discussion. At the beginning of the session, the individual readiness assurance test (iRAT) is given to students, and then they answer the same questions through discussion in a team. Finally, problems are resolved by the teacher and results are given to students. Methods: In the current study, 30 pharmacology students were randomly assigned to two groups of 16 and 14 subjects. The educational content of pharmaceutical biotechnology was given to the 16-and 14-subject groups using traditional lecturing and TBL methods, respectively. Finally, both groups were assessed and compared based on a same method.