Technical aspects of oocyte cryopreservation

Fabbri, Raffaella; Porcu, Eleonora; Marsella, Tiziana; Primavera, M.R.; Rocchetta, G.; Ciotti, P.M.; Magrini, Otello; Seracchioli, Renato; Venturoli, Stefano; Flamigni, Carlo

doi:10.1016/s0303-7207(00)00349-x

Cited by 59 publications

(29 citation statements)

References 19 publications

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“…Oocytes are also more susceptible to damaging effects of reactive oxygen species (Gupta et al 2010). Many of these parameters change after fertilization, making embryos less chilling sensitive and easier to cryopreserve (Gook et al 1993, Fabbri et al 2000, Ghetler et al 2005. Despite many advances in the field of cryopreservation, specifically with regards to oocytes (ovulated, mature or immature), their cryopreservation is still not considered an established procedure and thus its current label as experimental technique (Noyes et al 2010).…”

Section: Oocyte Cryopreservation: the Difficultiesmentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two basic cryopreservation techniques rule the field -controlled-rate freezing, the first to be developed, and vitrification, which, in recent years, has gained a foothold. While much progress has been achieved in human medicine, the cattle industry, and in laboratory animals, this is far from being the case for most other mammals and even less so for other vertebrates. The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.

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Section: Oocyte Cryopreservation: the Difficultiesmentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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“…Revivability of cryopreserved oocytes from small rodents and humans is extremely high, adapting well to the maintenance of huge number of transgenic strains and the efficient use in therapies for human infertility (Fabbri et al 2000). However, in bovine species, the fertilization rate and subsequent developmental competence of cryopreserved oocytes still need improvement (Ledda et al 2001).…”

Section: Introductionmentioning

confidence: 99%

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

et al. 2015

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The objective of this study was to investigate whether developmental competence of vitrified-warmed bovine oocytes can be improved by antioxidant treatment during recovery culture. In experiment 1, one of the two antioxidants (either L-ascorbic acid or a-tocopherol) was added as a supplement to the recovery culture medium to which postwarming oocytes were exposed for 2 h before IVF. The exposure to a-tocopherol had a positive effect on rescuing the oocytes as assessed by the blastocyst yield 8 days after the IVF (35.1-36.3% vs 19.2-25.8% in untreated postwarming oocytes). Quality of expanding blastocysts harvested on Day 8 was comparable between a-tocopherol-treated vitrification group and fresh control group in terms of total cell number and chromosomal ploidy. In experiment 2, level of reactive oxygen species, mitochondrial activity, and distribution of cortical granules in a-tocopherol-treated postwarming oocytes were assessed. No obvious differences from the control data were found in these parameters. However, the treatment with a-tocopherol increased the percentage of zygotes exhibiting normal single aster formation (90.3% vs 48.0% in untreated postwarming oocytes; 10 h post-IVF). It was concluded that a-tocopherol treatment of vitrified-warmed bovine mature oocytes during recovery culture can improve their revivability, as shown by the high blastocyst yield and the higher mean total cell number in the blastocysts.

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“…However, the larger volume and smaller surface-to-volume ratio of oocytes makes their cryopreservation more difficult, especially in mammals [2].…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods Freshly isolated (control group) and vitrifiedwarmed COCs (cryotop group) were matured in vitro. The expression of pro-and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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Technical aspects of oocyte cryopreservation

Cited by 59 publications

References 19 publications

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

High revivability of vitrified–warmed bovine mature oocytes after recovery culture with α-tocopherol

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

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