Technical Considerations for Developing Enzyme Immunohistochemical Staining Procedures on Formalin-Fixed Paraffin-Embedded Tissues for Diagnostic Pathology

Haines, Deborah M.; Chelack, Brian J.

doi:10.1177/104063879100300128

Cited by 207 publications

(185 citation statements)

References 26 publications

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“…7 Endogenous peroxidase was expended in deparaffinized sections and chamber slides by incubation in 3% hydrogen peroxide for 30 min and 5 min, respectively, at room temperature. Nonspecific binding was blocked by incubation with normal horse serum for 30 min at the dilution suggested by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

Paramyxovirus Infection in Caiman Lizards (Draecena Guianensis)

et al. 2001

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Abstract. Three separate epidemics occurred in caiman lizards (Dracaena guianensis) that were imported into the USA from Peru in late 1998 and early 1999. Histologic evaluation of tissues from necropsied lizards demonstrated a proliferative pneumonia. Electron microscopic examination of lung tissue revealed a virus that was consistent with members of the family Paramyxoviridae. Using a rabbit polyclonal antibody against an isolate of ophidian (snake) paramyxovirus, an immunoperoxidase staining technique demonstrated immunoreactivity within pulmonary epithelial cells of 1 lizard. Homogenates of lung, brain, liver, or kidney from affected lizards were placed in flasks containing monolayers of either terrapene heart cells or viper heart cells. Five to 10 days later, syncytial cells formed. When Vero cells were inoculated with supernatant of infected terrapene heart cells, similar syncytial cells developed. Electron microscopic evaluation of infected terrapene heart cells revealed intracytoplasmic inclusions consisting of nucleocapsid strands. Using negative-staining electron microscopy, abundant filamentous nucleocapsid material with a herringbone structure typical of the Paramyxoviridae was observed in culture medium of infected viper heart cells. Seven months following the initial epizootic, blood samples were collected from surviving group 1 lizards, and a hemagglutination inhibition assay was performed to determine presence of specific antibody against the caiman lizard isolate. Of the 17 lizards sampled, 7 had titers of Յ1:20 and 10 had titers of Ͼ1:20 and Յ1:80. This report is only the second of a paramyxovirus identified in a lizard and is the first to snow the relationship between histologic and ultrastructural findings and virus isolation.

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Section: Methodsmentioning

confidence: 99%

Paramyxovirus Infection in Caiman Lizards (Draecena Guianensis)

et al. 2001

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“…The tumors were stained for smooth actin a (ready-to-use solution), desmin a (1: 40 dilution), and vimentin b (1:100 dilution) using a standard immunohistochemical method. 6 The antibody reaction products were visualized with 3-3Ј-diaminobenzidine tetrachloride and counterstained with Mayer hematoxylin. Specificity of the immunoreactions was verified by staining negative and positive control tissue sections.…”

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confidence: 99%

Solitary Dermal Leiomyosarcomas in 12 Ferrets

Mikaelian

Garner

2002

J VET Diagn Invest

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Twelve 3–6-year-old ferrets (8 males, 3 females, 1 unknown) were presented with single cutaneous nodules. These dermal tumors were characterized histologically by nodular proliferation of neoplastic smooth muscle fibers with marked anisokaryosis and a mitotic rate of >2 mitoses per 10 high-power fields. Neoplastic cells stained strongly for vimentin in all tumors and for smooth muscle actin and desmin in all but 1 tumor. Histologic and immunohistochemical findings suggested a diagnosis of piloleiomyosarcoma for these tumors. Excision was curative in all animals available for follow-up. However, 3 of 5 animals developed adrenal disease within 7 months after removal of the dermal leiomyosarcoma.

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“…6 Utilization of formalin-fixed tissues provides the opportunity to preselect sections containing histologic evidence of uveal melanoma to be immunohistochemically examined for intralesional replication of FeLV or FeSV. 9 The majority of feline DIMs studied to date fail to demonstrate the presence of FeLV DNA (33/36 affected eyes 19 ; 10/10 affected eyes, present study) or antigen (10/10 affected eyes, present study). These results suggest that FeLV is not involved in the pathogenesis of the majority of these neoplasms.…”

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confidence: 66%

“…However, fixation of the globes using 10% neutral buffered formalin may result in diminished tissue antigenicity and DNA degradation. 9,19 Alternatively, FeLV/FeSV may initiate feline DIM but no longer be detectable during more advanced neoplastic stages that prompt enucleation of affected globes. 12 As with other neoplasms studied, feline DIMs are likely to develop through a multistep process involving a series of genetic insults to uveal melanocytes.…”

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confidence: 99%

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Lack of Detection of Feline Leukemia and Feline Sarcoma Viruses in Diffuse Iris Melanomas of Cats by Immunohistochemistry and Polymerase Chain Reaction

et al. 2002

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Abstract. Diffuse iris melanoma was confirmed by light-microscopic examination in 10 formalin-fixed, paraffin-embedded globes from 10 cats. To determine if feline leukemia virus or a replication defective feline leukemia virus, feline sarcoma virus, was present in these anterior uveal melanomas, immunohistochemistry and polymerase chain reaction for feline leukemia virus were utilized. Immunohistochemical staining for feline leukemia virus glycoprotein 70 was performed on all 10 tumors using an avidin-biotin complex technique. The DNA was extracted from each specimen and a 166-base pair region of the feline leukemia virus long terminal repeat was targeted by polymerase chain reaction. Immunohistochemical staining for feline leukemia virus glycoprotein 70 and polymerase chain reaction amplification of a feline leukemia virus long terminal repeat region were negative in all cases. Feline leukemia virus/feline sarcoma virus was not detected in any neoplasms and therefore was unlikely to play a role in the tumorigenesis of these feline diffuse iris melanomas.There are several reports in the literature of malignant diffuse iris melanomas (DIMs) in cats.

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Technical Considerations for Developing Enzyme Immunohistochemical Staining Procedures on Formalin-Fixed Paraffin-Embedded Tissues for Diagnostic Pathology

Cited by 207 publications

References 26 publications

Paramyxovirus Infection in Caiman Lizards (Draecena Guianensis)

Paramyxovirus Infection in Caiman Lizards (Draecena Guianensis)

Solitary Dermal Leiomyosarcomas in 12 Ferrets

Lack of Detection of Feline Leukemia and Feline Sarcoma Viruses in Diffuse Iris Melanomas of Cats by Immunohistochemistry and Polymerase Chain Reaction

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