Technical note: Bioluminescent bacterial test for acute toxicity: The effect of pH and buffer solutions

Andriano, Kirk P.; Taylor, Michelle S.; Felix, Brent A.; Hamber, Elizabeth A.; Daniels, A. U.; Heller, Jorge

doi:10.1002/jab.770060210

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…In the context of enhanced CL assays, it is important to note that buffers are not inert and should be considered as a crucial fourth component of the HRPenhanced CL light-generating system (35). Cercek et al (36) shows that, at the same pH and HRP concentration, the magnitude of the light signal (RLU) is significantly influenced by the reagent buffer composition, and could increase up to 10-fold, e.g.…”

Section: Immunoassaysmentioning

confidence: 99%

Chemiluminescent and bioluminescent assays as innovative prospects for mycotoxin determination in food and feed

Sarter

Zakhia

2004

Luminescence

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Mycotoxin contamination of food and feedstuffs is among the top priorities for human and animal safety. The currently used techniques for mycotoxin determination, either chromatography or ELISA, are unsuitable for routine in-field assessment. There is an urgent need for other accurate, simple and cost-effective techniques that can be used as a screening tool for a rapid estimation of mycotoxin contamination in commodity lots. This paper reviews the literature on the use of chemiluminescence (CL) and bioluminescence (BL) assays for direct or indirect mycotoxin assessment. The chemiluminescence immunoassays, adenosine triphosphate (ATP) bioluminescence and bioassays are reviewed and their advantages and limitations discussed. These techniques used in food testing and the pharmaceutical industry offer promise as rapid techniques for mycotoxin determination. Chemiluminescence and bioluminescence bioassays are the most innovative alternatives to the conventional techniques used for mycotoxin determination in food and feed.

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Section: Immunoassaysmentioning

confidence: 99%

Chemiluminescent and bioluminescent assays as innovative prospects for mycotoxin determination in food and feed

Sarter

Zakhia

2004

Luminescence

View full text Add to dashboard Cite

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How much do the conventional parameters contribute to the biological toxicity of surface water in different types of villages?

et al. 2021

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Background In rural areas, the surface water quality is seriously threatened by pollution from agriculture, breeding, industrial and tourism activities. Even though many strategies and programs were launched for rural environment management, it is challenging to tackle the serious surface water pollution in villages. Since pollution status varies with the type of villages, there is no single parameter that defines environment quality completely. Until recently, most of rural surface water quality monitoring programs focus only on the conventional parameters, while can these conventional parameters reflect comprehensive status of water quality? To study how much the conventional parameters contribute to biological toxicity of surface water in villages, the in situ parameters of pH, DO, EC, ORP, laboratorial parameters of COD, TN, NO3−-N, NO2−-N, NH4+-N, TP, and PO43−-P and acute toxicity were analyzed for the surface water samples those were collected from six types of villages, named breeding, agricultural, handicraft, industrial, agricultural and breeding, and tourism. The correlation analysis was applied to establish the linkages and contributions of each parameter. Results The results showed that all detected surface waters were alkaline, and the concentration of TN and most of COD concentrations in all six types of villages were higher than the Class V limits specified in environmental quality standards for surface water (GB3838-2002). Pearson’s correlation analysis showed that significantly positive correlations were found for the acute toxicity effects and laboratorial parameters of NO2−-N, NH4+-N and PO43−-P in agricultural villages, and negative correlations were found for the acute toxicity effects and laboratorial parameters of TN and NO3−-N. No significant correlation was observed in breeding villages and industrial villages. Conclusion The problems of nitrogen pollution and aerobic pollution are still serious in villages, and more attention should be paid in further rural environment management. NH4+-N could contribute to the acute toxicity of surface water in the most of investigated villages, while no significant correlation was observed between acute toxicity and conventional parameters in industrial villages and tourism villages. Environmental monitoring programs focusing just on the classical conventional parameters are far from sufficient, since the main toxic contributors are quite different in diverse villages. The outcomes of the present study contribute to demonstrate the performance and usefulness of bioanalytical techniques for water quality assessment.

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Technical note: Bioluminescent bacterial test for acute toxicity: The effect of pH and buffer solutions

Cited by 2 publications

References 3 publications

Chemiluminescent and bioluminescent assays as innovative prospects for mycotoxin determination in food and feed

Chemiluminescent and bioluminescent assays as innovative prospects for mycotoxin determination in food and feed

How much do the conventional parameters contribute to the biological toxicity of surface water in different types of villages?

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