Technical Note: Comparison of 4 methods for determining in vitro ruminal digestibility of annual ryegrass

Alende, M.; Lascano, G.J.; Jenkins, T.C.; Koch, L. E.; Volpi-Lagreca, Gabriela; Andrae, John

doi:10.15232/pas.2017-01688

Cited by 5 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The continuous shaking of samples produced significantly higher digestibility results than when shaking occurred only twice daily [72]. As reported by Alende et al [25], the use of filter bags may be advantageous, because filtration and recovery have been mentioned as sources of variability of the digestibility coefficients. Additionally, jars positioned horizontally render a higher digestibility than vertically placed ones.…”

Section: Sample Size Sample Weight and Bag Typementioning

confidence: 74%

“…Many methods are available to measure in vitro digestibility, but only a few articles have compared the results obtained using an AD II incubator with the results of other procedures [25].…”

Section: Comparison With Other Methodsmentioning

confidence: 99%

“…It has been identified as an easy, inexpensive, and efficient instrument for the prediction of the digestibility of several feedstuffs and diets [8,22]. However, compared to other techniques (such as the batch culture technique, the use of the Ankom Gas Production System or the Rumen Simulation Technique), the AD II incubator has been demonstrated to give higher values at different incubation times [25].…”

Section: The Ankom Daisy II Incubatormentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

Tassone

Fortina

Peiretti

2020

Animals

View full text Add to dashboard Cite

This review summarises the use of the Ankom DaisyII incubator (ADII; Ankom Technology Corporation Fairport, NY, USA), as presented in studies on digestibility, and its extension to other species apart from ruminants, from its introduction until today. This technique has been modified and adapted to allow for different types of investigations to be conducted. Researchers have studied and tested different procedures, and the main sources of variation have been found to be: the inoculum source, sample size, sample preparation, and bag type. In vitro digestibility methods, applied to the ADII incubator, have been reviewed, the precision and accuracy of the method using the ADII incubator have been dealt with, and comparisons with other methods have been made. Moreover, some hypotheses on the possible evolutions of this technology in non-ruminants, including pets, have been described. To date, there are no standardised protocols for the collection, storage, and transportation of rumen fluid or faeces. There is also still a need to standardise the procedures for washing the bags after digestion. Moreover, some performance metrics of the instrument (such as the reliability of the rotation mechanism of the jars) still require improvement.

show abstract

Section: Sample Size Sample Weight and Bag Typementioning

confidence: 74%

Section: Comparison With Other Methodsmentioning

confidence: 99%

Section: The Ankom Daisy II Incubatormentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

Tassone

Fortina

Peiretti

2020

Animals

View full text Add to dashboard Cite

show abstract

“…We used a dual-flow continuous culture fermentation system because continuous culture fermenters run continuously for longer periods than in the Daisy II , batch culture and the Ankon Gas Production System, furthermore, the continuous culture can mimic in vivo ruminal conditions and allows for natural stratification of feed particles, anaerobiosis, controlled temperature, and salivary buffering, similar to what occurs in the rumen (Alende et al 2018 ). A dual-flow continuous culture system (Adebayo Arowolo et al 2021 ) was used in this study.…”

Section: Methodsmentioning

confidence: 99%

Effects of different protein sources on nutrient disappearance, rumen fermentation parameters and microbiota in dual-flow continuous culture system

Ren

Zhu

et al. 2022

AMB Expr

View full text Add to dashboard Cite

Scarce high-quality protein feed resources has limited the development of animal husbandry. In this study, we used a dual-flow continuous culture system to evaluate effects of difference dietary protein sources including soybean meal (SBM), cottonseed meal (CSM), and rapeseed meal (RSM), on nutrient disappearance, rumen fermentation, and microbiota of XiongDong black goats. Dietary proteins of either CSM, RSM or SBM had no effect on nutrient disappearance (P > 0.05). CSM or RSM significantly reduced (P < 0.01) the pH and enhanced (P < 0.01) the ammonia nitrogen (NH3-N) concentration in fermentation liquid compared to SBM. The short-chain fatty acids (SCFAs) contents were greater (P = 0.05) and acetate was lower (P < 0.01) in SBM than those in RSM and CSM, whereas propionate was greater (P < 0.01) in RSM than that in SBM, consequently reducing the acetate to propionate ratio (A/P) in RSM. Bacteroidetes, Firmicutes, and Proteobacteria were detected as the dominant phyla, and the relative abundances of Spirochaetae (P < 0.01) and Chlorobi (P < 0.05) declined in the CSM and RSM groups as compared to those in the SBM group. At the genus level, Prevotella_1 was the dominant genus; as compared to SBM, its relative abundance was greater (P < 0.01) in CSM and RSM. The abundances of Prevotellaceae_Ga6A1 and Christensenellaceae_R7 were lower (P < 0.05) in CSM, whereas Eubacterium_oxidoreducens_group, and Treponema_2 were lower (P < 0.01) in both CSM and RSM, and other genera were not different (P > 0.10). Although the bacterial community changed with different dietary protein sources, the disappearances of nutrients were not affected, suggesting that CSM and RSM could be used by rumen bacteria, as in case with SBM, and are suitable protein sources for ruminant diets.

show abstract

“…The determination of fiber digestibility by in-vitro fermentation of feedstuff is applied in research and commercially to evaluate the nutritional value of fibrous feeds for livestock [1,2]. Although in-vivo determination fiber digestibility is regarded as standard for digestibility evaluation, it remains time consuming, expensive, and labor intensive [3]. Therefore, in-vitro techniques are highly used and are often correlated with in-vivo estimates [4].…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Fiber Sources on In-Vitro Fermentation and Microbiota in Monogastrics

Ngalavu

Jiang

El‐Ashram

et al. 2020

Animals

View full text Add to dashboard Cite

Feed fiber composition is usually considered as one of the factors that have an impact on digestive tract microbiota composition. The investigations on the level of fermentation and in-vitro digestibility of different fibers are not well understood. The aim of the current study is to determine the effect of different fiber sources on intestinal nutrient digestibility, hindgut fermentation, and microbial community composition under in vitro conditions using pigs’ hindgut as a model. The experimental treatment diets contained alfalfa hay, cornstalk, and rice straw. Cornstalk treatment displayed higher digestibility compared to alfalfa hay and rice straw; similar results were observed with in-vitro digestibility using intestinal digesta. Firmicutes were the most abundant phyla (Firmicutes = 89.2%), and Lactobacillus were the prominent genera (75.2%) in response to alfalfa compared to rice straw and cornstalk treatments. In simulated in-vitro digestion, corn stalk fiber improved dry matter digestibility; rice straw fiber improved volatile fatty acid content and fermentation efficiency. Alfalfa fiber improved the thickness of deposited Firmicutes and Lactobacillus.

show abstract

Technical Note: Comparison of 4 methods for determining in vitro ruminal digestibility of annual ryegrass

Cited by 5 publications

References 15 publications

In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

In Vitro Techniques Using the DaisyII Incubator for the Assessment of Digestibility: A Review

Effects of different protein sources on nutrient disappearance, rumen fermentation parameters and microbiota in dual-flow continuous culture system

Effect of Dietary Fiber Sources on In-Vitro Fermentation and Microbiota in Monogastrics

Contact Info

Product

Resources

About