2014
DOI: 10.3168/jds.2013-7208
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Technical note: Effects of frozen storage on the mechanical properties of the suspensory tissue in the bovine claw

Abstract: It is proposed that a softening of the suspensory tissue in the claw is involved in the development of lameness and claw lesions in cattle. A relatively small amount of research has been carried out to verify this theory. Research in this area would be simplified if mechanical testing of the suspensory tissue could be performed on frozen and stored specimens. The current study tested whether freezing of the specimens changes the suspensory tissues' mechanical properties. Limbs from 3 freshly slaughtered Danish… Show more

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Cited by 11 publications
(4 citation statements)
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“…No data are available regarding the effect of freezing on the mechanical properties of the supportive apparatus of the bovine claw. Boettcher et al (2014) and Tarlton and Webster (2000) reported that sample storage at either −18°C or −20°C had no effect on the biomechanical strength (Young's modulus) of the suspensory tissue in bovine claws, compared with fresh samples. Equally, Bennett and Ker (1990) showed similar mechanical properties between human heel pads tested within 1 h from harvesting or after frozen storage (temperature not specified).…”
Section: Discussionmentioning
confidence: 99%
“…No data are available regarding the effect of freezing on the mechanical properties of the supportive apparatus of the bovine claw. Boettcher et al (2014) and Tarlton and Webster (2000) reported that sample storage at either −18°C or −20°C had no effect on the biomechanical strength (Young's modulus) of the suspensory tissue in bovine claws, compared with fresh samples. Equally, Bennett and Ker (1990) showed similar mechanical properties between human heel pads tested within 1 h from harvesting or after frozen storage (temperature not specified).…”
Section: Discussionmentioning
confidence: 99%
“…While the snap freezing and sectioning required in this method may alter mechanical properties, causing differences as compared to the in vivo state, snap freezing allows for long-term tissue storage, more uniform and thin sectioning, and has been widely used in the biomedical research field (Graham et al, 2010;Peña et al, 2022;Usukura et al, 2017;Wang et al, 2017). Rapid freezing and thawing has been shown to preserve biomechanical properties of tissue sections, and a consistent experimental protocol still allows researchers to compare the effects of different biological conditions or sample locations on tissue mechanical properties (Boettcher et al, 2014;Calò et al, 2020;Lopez et al, 2011;Tran et al, 2017). AFM force-displacement measurements have thus been performed on cryosections of various tissue types, including brain, heart, lens, cornea, retina, trabecular meshwork/Schlemm's canal, and optic nerve (Franze et al, 2011;Last et al, 2010;Menal et al, 2018;Perea-Gil et al, 2015;Vahabikashi et al, 2019;Wang et al, 2018Wang et al, , 2017.…”
Section: Introductionmentioning
confidence: 99%
“…The residual soft tissue was removed and the tibias were stored in phosphatebuffered saline solution and frozen at -18 °C for 24 h before testing. For the dental implant insertion the tibias were removed from the frozen and stored on the bench for 2 h into the phosphate-buffered saline solution (21).…”
Section: Methodsmentioning
confidence: 99%