Technical report: Laser microdissection of cochlear structures from celloidin embedded human temporal bone tissues and detection of the mitochondrial DNA common deletion using real time PCR

Mitochondrial Damage in the Trabecular Meshwork of Patients With Glaucoma

“…By comparison, the level of mtDNA 4977 as detected by our group using the herein-reported QPCR method in stemlike mammary cells spanned from 0.9% to 2.1% only. 35 Markaryan et al 15 detected a similar range of molecular lesions in different tissues of cochlear structures using the same method.…”

“…13 The quantification of mtDNA 4977 was carried out by quantitative polymerase chain reaction (QPCR), which is highly sensitive and reliable as confirmed by the increasing number of articles using this method. 14,15 Insofar, evidence demonstrating the purported role of mitochondrial damage in POAG pathogenesis is overwhelming, but most results were not obtained using POAG target tissue, ie, the TM. Our study provides for the first time, to our knowledge, evidence that mitochondrial damage really occurs in the TM of patients with glaucoma, thus providing evidence for its pathogenic role in glaucoma.…”

Quantitative cellular level analysis of mitochondrial DNA 3243A > G mutations in individual tissues from the archival temporal bones of a MELAS patient

“…Moreover, advanced techniques have made it possible to isolate, amplify and analyse both DNA and RNA from samples of human archival celloidin-embedded, paraffin-embedded and frozen sections of temporal bone [5][6][7][8]11]. In the present study, we combined LCM and real-time PCR to selectively analyse the mtDNA 3243A > G mutation rate in cells from specific organs within the inner ear of a MELAS patient and correlated those findings with the histopathological findings.…”

“…Using LCM and real-time PCR, Markaryan et al also were able to quantitatively assess common mtDNA deletions in specimens of inner ear tissue from archival human temporal bone sections [8]. Until now, however, there has been no study in which a quantitative analysis of the mutation rate in deafness genes (e.g.…”

Protective roles of α-lipoic acid in rat model of mitochondrial DNA4834bp deletion in inner ear

“…The symptoms of these diseases include hearing loss. The mtDNA4977bp deletion in humans (a similar mtDNA4834bp deletion in rats) is commonly found in diverse senescent tissues [12] . Furthermore, the percentage of this deletion was found to increase with aging [13] .…”