Technique for preparing appendage-free skin (Scar) on hairless mouse

Behl, Charanjit R.; Wittkowsky, Ann; Barrett, Michael T.; Pierson, Carl L.; Flynn, Gordon L.

doi:10.1002/jps.2600700742

Cited by 11 publications

(2 citation statements)

References 11 publications

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“…The first quantitative studies were done by Illel, Doucet and their collaborators [11][12][13][14]. Illel and Schaefer [11] used a technique described by Behl et al [ 15] on the hairless mouse to produce appendagefree skin in the hairless rat. The observation has been made that in vitro percutaneous absorption of several drugs was lower in ap pendage-free than in normal skin.…”

Section: Introductionmentioning

confidence: 99%

Role of Transepidermal and Transfollicular Routes in Percutaneous Absorption of Hydrocortisone and Testosterone: In vivo Study in the Hairless Rat

Hueber

Wépierre

Schaefer

1992

Skin Pharmacol Physiol

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The importance of the transappendageal route on percutaneous absorption was assessed in the hairless rat. Skin permeation of two steroids, hydrocortisone and testosterone, was evaluated in vivo on normal and artificially damaged skin in which follicles and sebaceous glands disappeared during healing. The test compounds were applied for periods of 0.5,2 and 6 h. Thereafter, the stratum corneum reservoir function, the epidermal and dermal distribution profiles, and systemic absorption were determined for both molecules. The results presented here show that the reservoir function of the stratum corneum of appendage-free (scar) skin is more pronounced than that of normal skin, whereas the concentration appearing in the epidermis and dermis was greater in normal skin. Moreover, sebaceous glands probably contribute to the penetration of hydrocortisone and testosterone. We show that the relative importance of the skin appendages depends on the intrinsic physical properties of the molecules tested, and the time of application.

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Section: Introductionmentioning

confidence: 99%

Role of Transepidermal and Transfollicular Routes in Percutaneous Absorption of Hydrocortisone and Testosterone: In vivo Study in the Hairless Rat

Hueber

Wépierre

Schaefer

1992

Skin Pharmacol Physiol

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“…The problem of this approach is that skin with different hair follicle densities may also have other structural properties. Moreover, a comparison of scarred skin [9] and normal skin is rather critical to evaluate, since the barrier properties of the skin are certainly not comparable.…”

Section: Introductionmentioning

confidence: 99%

Solvent-Containing Closure Material Can Be Used to Prevent Follicular Penetration of Caffeine and Fluorescein Sodium Salt on Porcine Ear Skin

Klein

Lubda

Taghavi

et al. 2020

Skin Pharmacol Physiol

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Aim: The skin represents a drug delivery portal. The establishment of a skin model capable of distinguishing between the follicular and intercellular penetration pathways remains a challenge. The study described herein was aimed at showing the influence of two nail varnishes as closure material and four application techniques to spread the active pharmaceutical ingredient (API) on a successful follicular closure without inducing penetration-enhancing effects. Materials and Methods: For all experiments, ex vivo porcine ear skin was used. In study design A, a standard and a solvent-free nail varnish were compared. It was tested whether the different application techniques (spreading with pipette, careful finger massage, 5-Hz finger massage, 5-Hz automatic massage) potentially destroy an intact follicular closure. Laser scanning microscopy imaging was used to measure if the model drug (fluorescein sodium salt) penetrated into the Anna Lena Klein and Markus Lubda share first authorship. Klein et al.study clearly demonstrates that a standard nail varnish in combination with a gentle application technique of the API provides a secure follicular closure. The presented study only investigated the closure for the substances caffeine and fluorescein sodium salt. The results might not be transferable to all kinds of APIs.

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Investigation of transfollicular caffeine penetration using microdialysis on ex vivo porcine ear skin

Klein

Lubda

Skov

et al. 2020

European Journal of Pharmaceutics and Biopharmaceutics

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Technique for preparing appendage-free skin (Scar) on hairless mouse

Cited by 11 publications

References 11 publications

Role of Transepidermal and Transfollicular Routes in Percutaneous Absorption of Hydrocortisone and Testosterone: In vivo Study in the Hairless Rat

Role of Transepidermal and Transfollicular Routes in Percutaneous Absorption of Hydrocortisone and Testosterone: In vivo Study in the Hairless Rat

Solvent-Containing Closure Material Can Be Used to Prevent Follicular Penetration of Caffeine and Fluorescein Sodium Salt on Porcine Ear Skin

Investigation of transfollicular caffeine penetration using microdialysis on ex vivo porcine ear skin

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