Techniques for Neurochemical Research on the Retina

Voaden, Mary J.

doi:10.1007/978-1-4615-7760-7_1

Cited by 2 publications

(1 citation statement)

References 182 publications

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“…Mixed isomers of retinal (principally 13-cis, 11-cis, 9-cis, and all-trans), prepared by photoisomerization of the all-trans form [3], were encapsulated in phosphatidyl choline liposomes by means of sonication before application to a retina [4,5]. Only the 11-cis and 9-cis isomers of retinal form photoreactive complexes with opsin, yielding rhodopsin and isorhodopsin, respectively.…”

Section: Methodsmentioning

confidence: 99%

Electrophysiologic characteristics of human and rat retinas in vitro

et al. 1990

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To promote studies on the human retina, we investigated the survival of function in postmortem specimens. Visual pigment has been regenerated in normal human retinas, 5 to 58 hours postmortem, by exposure to retinal isomers in the dark. Levels from 0.1 to 0.41 nmol/mg protein were reached. Photoresponses were obtained in 9 of 13 retinas: P III maximum amplitudes ranged from 20-398 microV and thresholds, taking the criterion amplitude as 3 microV, ranged from 8.8-1340 quanta/micros2. In three cases, the b-wave was also seen. The P III amplitude vs. log intensity curves gave values of n between 0.6 and 1.0, and sigma (the stimulus intensity for a half maximal response) between 132-3700 quanta/microns2. Recovery of sensitivity did not always correspond to that of maximum response.

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Section: Methodsmentioning

confidence: 99%

Electrophysiologic characteristics of human and rat retinas in vitro

et al. 1990

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Survival of structure and function in postmortem rat and human retinas: rhodopsin regeneration, cGMP and the ERG

Huang

Voaden

Marshall

1990

Current Eye Research

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Procedures for regenerating visual pigment and restoring phototransduction have been established with freshly isolated, bleached rat retinas. Phosphatidyl choline liposomes containing a 500 microM mixture of retinal isomers, including the 9-cis and 11-cis forms, were employed and the results compared with dark-adapted retinas, incubated similarly but without retinal. The following were recovered in a 60 min incubation, rhodopsin (plus isorhodopsin) to 91% of the original rhodopsin concentration, 87% of cGMP and 89% of PIII amplitude at saturation. PIII amplitude vs. log intensity curves gave values of n between 0.6 and 1/2.0 and sigma between 85 and 439 quanta/micron 2. Human retinas, ranging from 18 to 58 hours postmortem and treated as above, also produced photoresponses. Of the 7 retinas studied so far, rhodopsin has been regenerated to 0.1-0.35 nmol/mg protein, cGMP to 23.5-49.2 pmol/mg protein, and PIII to 20-50 microV: in some cases a b-wave was also seen. Values of n varied between 0.6 and 1.0, and sigma between 132 and 3700 quanta/micron 2. PIII responses were also seen after retinas, approximately 30 hours postmortem, were incubated for a further 24 hours in fortified medium. After incubation, retinal vacuolation was reduced.

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Techniques for Neurochemical Research on the Retina

Cited by 2 publications

References 182 publications

Electrophysiologic characteristics of human and rat retinas in vitro

Electrophysiologic characteristics of human and rat retinas in vitro

Survival of structure and function in postmortem rat and human retinas: rhodopsin regeneration, cGMP and the ERG

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