Techniques for the optimum recovery of cold injured <i>Campylobacter jejuni</i> from milk or water

Humphrey, Tom J.

doi:10.1111/j.1365-2672.1986.tb04265.x

Cited by 75 publications

(64 citation statements)

References 12 publications

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“…Thus, the viability of C. jejuni in water under these conditions may decline rapidly. In addition, the methods necessary for recovery from water contribute to the physical damage and reduced recoverability (49).…”

Section: Sources Of Poultry Colonizationmentioning

confidence: 99%

Sources of Campylobacter Colonization in Broiler Chickens

Newell¹,

Fearnley²

2003

Appl Environ Microbiol

536

455

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Section: Sources Of Poultry Colonizationmentioning

confidence: 99%

Sources of Campylobacter Colonization in Broiler Chickens

Newell¹,

Fearnley²

2003

Appl Environ Microbiol

536

455

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“…taining the quenching agents ferrous sulphate, sodium pyruvate and sodium metabisulphite (Humphrey 1986).…”

Section: Introductionmentioning

confidence: 99%

Aerobic growth and survival of Campylobacter jejuni in food and stream water

Chynoweth

Hudson

Thom

1998

Lett Appl Microbiol

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When 40 Campylobacter jejuni isolates from human clinical cases, raw chicken and water were tested, 29 (72·5%) could be adapted to grow on nutrient agar under aerobic conditions. Once adapted, these isolates could grow on repeated aerobic subculture. An aerobically‐grown Camp. jejuni isolate survived almost as well as the same isolate grown microaerophilically in sterile chicken mince at 5 °C, and survival of a cocktail of Camp. jejuni isolates under both atmospheres was comparable at 25 °C. However, at 37 °C, the decline in numbers of the aerobically‐grown cells was greater. Survival of cells on chicken nuggets was poorer than in chicken mince. In filter‐sterilized stream water incubated aerobically at 5 °C, survival of inocula grown under different atmospheres was again similar, but slightly better with the microaerophilically‐grown cells. Adaptation to aerobic growth was not found to enhance survival under aerobic conditions.

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“…Preenrichment at 37°C for 2 to 4 h before exposure to selective agents and incubation at 42°C has also been reported as a recovery method that resulted in an increased isolation of Campylobacter spp. from natural waters (33)(34)(35)37). Many previous studies on the occurrence of Campylobacter spp.…”

Section: Discussionmentioning

confidence: 99%

“…in aquatic environments, where species such as C. jejuni and C. lari could be present in small numbers (26,27). Another approach in water studies has been to have a 2-to 4-h preenrichment step at 37°C before subsequent enrichment at 42°C (33)(34)(35)(36)(37)(38). However, studies that used a preenrichment step of 37°C followed by enrichment at 42°C have often observed a low detection rate for C. coli, suggesting that a 42°C incubation temperature might not support the growth of stressed and injured cells of C. coli present in small numbers in water.…”

mentioning

confidence: 99%

Effect of Incubation Temperature on the Detection of Thermophilic Campylobacter Species from Freshwater Beaches, Nearby Wastewater Effluents, and Bird Fecal Droppings

Khan

Hill

Nowak

et al. 2013

Appl Environ Microbiol

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b This large-scale study compared incubation temperatures (37°C versus 42°C) to study the detection of thermophilic Campylobacter species, including Campylobacter jejuni, C. coli, and C. lari, in various surface water samples and bird fecal droppings around Hamilton Harbor, Lake Ontario. The putative culture isolates obtained from incubation temperatures of 37 and 42°C were confirmed by Campylobacter genus-and species-specific triplex PCR assays targeting the 16S rRNA gene and the 16S-23S rRNA gene internal transcribed spacer (ITS) region. A total of 759 water, wastewater, and bird fecal dropping samples were tested. Positive amplification reactions for the genus Campylobacter were found for 454 (60%) samples incubated at 37°C, compared to 258 (34%) samples incubated at 42°C. C. jejuni (16%) and C. lari (12%) were detected significantly more frequently at the 42°C incubation temperature than at 37°C (8% and 5%, respectively). In contrast, significantly higher rates of C. coli (14%) and other Campylobacter spp. (36%) were detected at the 37°C incubation temperature than at 42°C (8% and 7%, respectively). These results were consistent across surface water, wastewater, and bird fecal dropping samples. At times, Campylobacter spp. were recovered and detected at 37°C (3% for C. jejuni, 10% for C. coli, and 3% for C. lari) when the same samples incubated at 42°C were negative. A significantly higher rate of other Campylobacter spp. was detected only at 37°C (32%) than only at 42°C (3%). These results indicate that incubation temperature can significantly influence the culturability and detection of thermophilic and other fastidious Campylobacter spp. and that a comprehensive characterization of the Campylobacter spp. in surface water, wastewaters, or bird fecal droppings will require incubation at both 37 and 42°C.

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Techniques for the optimum recovery of cold injured Campylobacter jejuni from milk or water

Cited by 75 publications

References 12 publications

Sources of Campylobacter Colonization in Broiler Chickens

Sources of Campylobacter Colonization in Broiler Chickens

Aerobic growth and survival of Campylobacter jejuni in food and stream water

Effect of Incubation Temperature on the Detection of Thermophilic Campylobacter Species from Freshwater Beaches, Nearby Wastewater Effluents, and Bird Fecal Droppings

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