Conventional phototrophic cultivation for microalgae
production
suffers from low and unstable biomass productivity due to limited
and unreliable light transmission outdoors. Alternatively, the use
of a renewable lignocellulose-derived carbon source, cellulosic hydrolysate,
offers a cost-effective and sustainable pathway to cultivate microalgae
heterotrophically with high algal growth rate and terminal density.
In this study, we evaluate the feasibility of cellulosic hydrolysate-mediated
heterotrophic cultivation (Cel-HC) for microalgae production by performing
economic and environmental comparisons with phototrophic cultivation
through techno-economic analysis and life cycle assessment. We estimate
a minimum selling price (MSP) of 4722 USD/t for producing high-purity
microalgae through Cel-HC considering annual biomass productivity
of 300 t (dry weight), which is competitive with the conventional
phototrophic raceway pond system. Revenues from the lignocellulose-derived
co-products, xylose and fulvic acid fertilizer, could further reduce
the MSP to 2976 USD/t, highlighting the advantages of simultaneously
producing high-value products and biofuels in an integrated biorefinery
scheme. Further, Cel-HC exhibits lower environmental impacts, such
as cumulative energy demand and greenhouse gas emissions, than phototrophic
systems, revealing its potential to reduce the carbon intensity of
algae-derived commodities. Our results demonstrate the economic and
environmental competitiveness of heterotrophic microalgae production
based on renewable bio-feedstock of lignocellulose.