Technologies Applied to Stimulate Bone Regeneration

Santos, Arnaldo Rodrigues; Lombello, Christiane Bertachini; Genari, Selma Candelária

doi:10.5772/26412

Cited by 6 publications

(2 citation statements)

References 143 publications

(136 reference statements)

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“…Bone repair is a complex process that consists of the stabilization of bone fragments, bone consolidation, reconstruction of avascular and seminecrotic fragments, and finally, internal and external remodeling of the newly formed tissue 1, 2. External factors can markedly affect the regeneration process, although tissues act according to biological rules that control cell proliferation and differentiation, as well as extracellular matrix production, which can independently occur despite of external interference, although being influenced by them 3. In contrast, fractures accompanied by bone mass loss require grafts or implants.…”

Section: Introductionmentioning

confidence: 99%

“…Cell interactions with biomaterials, as well as the quality of these interactions, are known to influence the ability of cells to proliferate and differentiate when in contact with the implant 21, 22. These interactions are influenced by the topography of the material, superficial energy, and roughness, among others 3, 6. Among many reports relating negative charges with osteogenesis, there is a few in vitro data that could help explain the effect of negative charges on differentiation of bone cells.…”

Section: Introductionmentioning

confidence: 99%

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Bovine osteoblasts cultured on polyanionic collagen scaffolds: An ultrastructural and immunocytochemical study

et al. 2012

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Collagen is the most abundant protein in the body and is also the most important component of the extracellular matrix. Collagen has several advantages as a biomaterial such as lack of toxicity, biocompatibility, biodegradability, and easy reabsorption. In this study, we examined bovine osteoblasts cultured on native or anionic collagen scaffolds prepared from bovine pericardium after selective hydrolysis of glutamine and asparagine side chain amides for periods from 24 (BP24) and 48 h (BP48). The cells were cultured in control and mineralization medium at 37 °C in the presence of 5% CO(2). Transmission and scanning electron microscopy, energy dispersive spectroscopy, and an immunocytochemical marker were used for analysis. Cells with an irregular morphology forming a confluent multilayer were observed on matrices kept in control medium. Most of these cells presented a polygonal or elongated flattened morphology. Several spherical deposits of calcium crystal associated with phosphorus were observed on the native and BP48 matrices. Similar results were observed in samples kept in control medium except with lower calcium/phosphorus ratio. Vesicles actively expelled from the cell membrane were also seen (do this vesicles corresponds to calcium/phosphorus deposits). Osteocalcin was clearly visible on matrices kept in mineralization medium and was more expression on the surface of BP48 matrices. The results showed that anionic collagen is able to support osteoblastic differentiation, regardless of the medium used. Finally, the BP48 matrix promoted better osteoblast differentiation than the native matrix.

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Section: Introductionmentioning

confidence: 99%