Technology and analysis of temperature-sensitive liposomal preparation of doxorubicin

Abstract

The optimum composition of temperature-sensitive liposomes (TSLs) has been chosen and a method of doxorubicin loading in TSLs by ammonium ion gradient has been developed. The TSL-encapsulated doxorubicin was lyophilized for better stabilization with 4.0% sucrose added as a cryoprotective agent. A method based on spectrophotometry at 252 nm is proposed for the determination of oxorubicin concentration in TSLs. It is established that TSLs encapsulate 89.5 ± 3.5% of doxorubicin. The particle size of the vesicles is 165 ± 10 nm. The proposed TSL doxorubicin formulation possesses selective action, shows higher efficiency and lower toxicity in comparison to the traditional doxorubicin, and can be recommended for use in the chemo-thermotherapy of solid tumors.

Study of the Efficiency of Doxorubicin Deposited in Microparticles from Resorbable BioplastotaneTM on Laboratory Animals with Ehrlich’s Solid Carcinoma

“…The experimental form of nanosomal doxorubicin, deposited in biodegraded polybutylcyanoacrylate particles, modifi ed by polysorbate 80, led to tumor growth inhibition, reduced cell proliferation and density of vessels [5]. Thermosensitive liposomes exhibited a pronounced cytotoxic effect in vitro and in vivo and selectively transported doxorubicin to the tumor [1]. Moreover, the developed doxorubicin forms were characterized by a lesser cardiotoxicity in comparison with the standard drug [4,9].…”

“…In order to reduce its toxicity and increase its specifi c activity, new dosage forms are created: polymeric micro-and nanoparticles, nanosomal forms, liposomes, thermostable conjugates with polypeptides, polymeric micelles with transverse ionic bonds, and micelles with chemical bonds easily cleaved in acid medium and promoting release of free doxorubicin [1,5,10,12]. The possibility of doxorubicin conjugation with and binding to various substrates is the key prerequisite for its deposition in polymeric matrix.…”

Use of Membrane Filters in Production Technology for Sterile Drugs

“…The integrity of the pore structure of membranes based on cellulose derivatives can be disrupted during filtration, such that the sterility of the resulting solution cannot be assured. Sterilizing filtration of BCNU solutions should therefore use filters based on polyvinylidene difluoride (GVHP), capron (Mifil), or analogous materials [24].…”

The Effect of Liposomes of Various Compositions on the Skin and Its Derivatives After II–IIIA Degree Thermal Burns

“…Currently, liposomes are widely used as biological nanocontainers for drug delivery in oncology [ 27 , 28 ], ophthalmology [ 29 ], dermatology [ 30 ], gene therapy [ 31 ], and other fields of medicine. The undeniable advantages of liposomes include their biodegradability, low immunogenicity, and ability to interact with the cell membrane, ensuring intracellular delivery of their contents [ 32 ]. However, despite the evidence of high metabolic activity of essential PUFAs constituting the phospholipids used for the production of liposomes, not enough attention is focused on the metabolic effects of such liposomes, depending on the spectrum of fatty acids included in their composition.…”