Telemetric monitoring of bladder function in female Göttingen minipigs

Abstract

These authors contributed equally to this work. ObjectivesTo generate real-time radio-telemetric urodynamic reference data of maximum detrusor pressure (P det max ), maximum flow rate (Q max) and estimated grade of infravesical obstruction, as well as duration of detrusor contraction (DOC), in female G€ ottingen minipigs and to describe translational aspects of the use of G€ ottingen minipigs for urological research. Materials and MethodsA telemetric transmitter was implanted into five female G€ ottingen minipigs, and 24 h measurements in metabolic cages were taken. Through operator-based analysis, synchronized real-time radio-telemetric cystometric data with flowmetric data and video sequences were used to determine voiding detrusor contractions (VCs) and non-voiding detrusor contractions (NVCs). Furthermore, data from telemetric natural-filling cystometry from free-moving and restricted maintenance conditions were compared for potential differences. ResultsThe median (range) P det max of VCs was 120.6 (21.0-370.0) cmH 2 O and, therefore, significantly different from that of .6 cmH 2 O] cm H 2 O). Intraindividual comparison of minipig data revealed great differences in voiding contractions. The effects of limited movement on VCs were analysed and showed significantly higher P det max and lower DOC than in free-moving conditions. ConclusionThe presented data can be used for the development of telecystometric implanted minipig models, to investigate changes of detrusor function such as under-or overactivity, and might serve as model for bladder changes occurring with iatrogenic bladder outlet obstruction (BOO) or different therapeutic options for overactive bladder. Radio-telemetric real-time natural filling and voiding cystometries are feasible, reproducible in non-anaesthetized minipigs with free or limited movement and can give new insights into circadian behaviour and physiological and pathological bladder function.

Bladder telemetry: A new approach to evaluate micturition behavior under physiological and inflammatory conditions

“…A similar approach has been reported in beagle dogs 10 or in minipigs. 11 If such telemetric techniques can be applied to small animal models in which pathophysiological conditions such as interstitial cystitis or chronic bladder pain symptoms could be induced, this would represent a unique tool for in vivo pharmacology studies, and for a better understanding of micturition behaviors.…”

Novel implantable pressure and acceleration sensor for bladder monitoring

“…Pressure measurement instruments can be placed either within the bladder lumen or in the bladder submucosa. Intraluminal devices might require an opening of the bladder mucosa and carry the risk of calculus formation 18 . Submucosal implantation avoids direct contact with urine, which reduces the risk of encrustation while placing tissue overlying the sensing membrane of the pressure microchip 11 .…”

Two differentially structured collagen scaffolds for potential urinary bladder augmentation: proof of concept study in a Göttingen minipig model

“…This monitoring was also done prior to and after each operation as well as the termination of the experiment to evaluate the bladder function. Proof of the time point and volume of the micturition was ensured by video monitoring (Mobotix, Langmeil, Germany) and an urological flow meter consisting of a scale with a collecting vessel and corresponding software (Flow, Laborie, Montreal, Quebec, Canada) as described elsewhere [36]. …”