Telemetric Recording of Sleep and Home Cage Activity in Mice

Tang, Xiangdong; Sanford, Larry D.

doi:10.1093/sleep/25.6.677

Cited by 90 publications

(68 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sleep and wakefulness of the animals were visually scored by a trained observer based on EEG and activity in 10-s epochs using a scoring program (Sleep Sign for Animal, Kissei, Nagano, Japan) as described previously. 25,26 Briefly, each epoch was scored as wakefulness with moving (AW, with activity recorded in epoch), quiet wakefulness (QW, no activity during epoch), NREM, or REM. During NREM sleep, the EEG was characterized as high amplitude slow waves, while REM was characterized as regularly spaced lower amplitude wave and increased theta activity.…”

Section: Cortical Eeg Recording and Determination Of Behavioral Statementioning

confidence: 99%

Brief Optogenetic Inhibition of the Basolateral Amygdala (BLA) in Mice Alters Effects of Stressful Experiences on Rapid Eye Movement Sleep (REM)

Machida

Wellman

et al. 2017

Sleep

View full text Add to dashboard Cite

Study Objectives: Stressful events can directly produce significant alterations in subsequent sleep, in particular rapid eye movement sleep (REM); however, the neural mechanisms underlying the process are not fully known. Here, we investigated the role of the basolateral nuclei of the amygdala (BLA) in regulating the effects of stressful experience on sleep. Methods: We used optogenetics to briefly inhibit glutamatergic cells in BLA during the presentation of inescapable footshock (IS) and assessed effects on sleep, the acute stress response, and fear memory. c-Fos expression was also assessed in the amygdala and the medial prefrontal cortex (mPFC), both regions involved in coping with stress, and in brain stem regions implicated in the regulation of REM. Results: Compared to control mice, peri-shock inhibition of BLA attenuated an immediate reduction in REM after IS and produced a significant overall increase in REM. Moreover, upon exposure to the shock context alone, mice receiving peri-shock inhibition of BLA during training showed increased REM without altered freezing (an index of fear memory) or stress-induced hyperthermia (an index of acute stress response). Inhibition of BLA during REM under freely sleeping conditions enhanced REM only when body temperature was high, suggesting the effect was influenced by stress. Peri-shock inhibition of BLA also led to elevated c-Fos expression in the central nucleus of the amygdala and mPFC and differentially altered c-Fos activity in the selected brain stem regions. Conclusions: Glutamatergic cells in BLA can modulate the effects of stress on REM and can mediate effects of fear memory on sleep that can be independent of behavioral fear.

show abstract

Section: Cortical Eeg Recording and Determination Of Behavioral Statementioning

confidence: 99%

Brief Optogenetic Inhibition of the Basolateral Amygdala (BLA) in Mice Alters Effects of Stressful Experiences on Rapid Eye Movement Sleep (REM)

Machida

Wellman

et al. 2017

Sleep

View full text Add to dashboard Cite

show abstract

“…After a 1-day acclimation and recovery period, the telemetry EEG/EMG devices were activated and continuous recordings were obtained for a total of 24 h (6 pm to 6 pm), as previously described. [37][38][39] EEG and EMG data were analyzed in 1-min epochs using Neuroscore software devices (Data Sciences International). Using both manual scoring as well as automated software, EEG/EMG recordings were broken down into active wake, non-rapid eye movement NREM sleep, and REM sleep.…”

Section: Assessment Of Sleep Behaviormentioning

confidence: 99%

“…REM sleep was classified as low-amplitude EEG with low EMG activity. [37][38][39] In addition to total sleep/wake time, power band (i.e., delta, theta, alpha, and beta) and power spectral (frequency) analysis of sleep/wake states were further assessed to study the quality of NREM and REM sleep in each animal. 40 …”

Section: Assessment Of Sleep Behaviormentioning

confidence: 99%

The Spectrum of Neurobehavioral Sequelae after Repetitive Mild Traumatic Brain Injury: A Novel Mouse Model of Chronic Traumatic Encephalopathy

Petraglia

Plog

Dayawansa

et al. 2014

Journal of Neurotrauma

198

194

View full text Add to dashboard Cite

There has been an increased focus on the neurological sequelae of repetitive mild traumatic brain injury (TBI), particularly neurodegenerative syndromes, such as chronic traumatic encephalopathy (CTE); however, no animal model exists that captures the behavioral spectrum of this phenomenon. We sought to develop an animal model of CTE. Our novel model is a modification and fusion of two of the most popular models of TBI and allows for controlled closed-head impacts to unanesthetized mice. Two-hundred and eighty 12-week-old mice were divided into control, single mild TBI (mTBI), and repetitive mTBI groups. Repetitive mTBI mice received six concussive impacts daily for 7 days. Behavior was assessed at various time points. Neurological Severity Score (NSS) was computed and vestibulomotor function tested with the wire grip test (WGT). Cognitive function was assessed with the Morris water maze (MWM), anxiety/risk-taking behavior with the elevated plus maze, and depression-like behavior with the forced swim/tail suspension tests. Sleep electroencephalogram/electromyography studies were performed at 1 month. NSS was elevated, compared to controls, in both TBI groups and improved over time. Repetitive mTBI mice demonstrated transient vestibulomotor deficits on WGT. Repetitive mTBI mice also demonstrated deficits in MWM testing. Both mTBI groups demonstrated increased anxiety at 2 weeks, but repetitive mTBI mice developed increased risk-taking behaviors at 1 month that persist at 6 months. Repetitive mTBI mice exhibit depression-like behavior at 1 month. Both groups demonstrate sleep disturbances. We describe the neurological sequelae of repetitive mTBI in a novel mouse model, which resemble several of the neuropsychiatric behaviors observed clinically in patients sustaining repetitive mild head injury.

show abstract

“…Telemeters (model # ETA10-F20, Data Sciences International, St. Paul, MN) to record the electroencephalogram (EEG) and core body temperature were surgically implanted in the peritoneal cavity under isoflurane anesthesia (4% induction, 2% maintenance) using sterile technique, as previously described (Tang and Sanford, 2002;Morrow and Opp, 2005b). Transmitter leads were passed subcutaneously to the base of the skull, where they were attached to stainless steel screws that served as EEG recording electrodes.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Effects of i.c.v. administration of interleukin-1 on sleep and body temperature of interleukin-6-deficient mice

Olivadoti

Opp

2008

Neuroscience

View full text Add to dashboard Cite

Cytokines in brain contribute to the regulation of physiological processes and complex behavior, including sleep. The cytokines that have been most extensively studied with respect to sleep are interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and IL-6. Administration of these cytokines into laboratory animals, or in some cases into healthy human volunteers, increases the amount of time spent in non-rapid eye movement (NREM) sleep. Although antagonizing the IL-1 or TNF systems reduces the amount of time laboratory animals spend in NREM sleep, interactions among these three cytokine systems as they pertain to the regulation of physiological NREM sleep are not well understand. To further elucidate mechanisms in brain by which IL-1β, TNFα, and/or IL-6 contribute to NREM sleep regulation, we injected recombinant murine IL-1β (muIL-1β) into C57BL/6J mice and into IL-6-deficient mice (IL-6 knockout, KO). IL-6 KO (B6.129S6-Il6 tm1Kopf ; n = 13) and C57BL/6J mice (n = 14) were implanted with telemeters to record the electroencephalogram (EEG) and core body temperature, as well as with indwelling guide cannulae targeted to one of the lateral ventricles. After recovery and habituation, mice were injected intracerebroventricularly (ICV) just prior to dark onset on different days with either 0.5 µl vehicle (pyrogen-free saline; PFS) or with 0.5 µl PFS containing one of four doses of muIL-1β (2.5 ng, 5 ng, 10 ng, 50 ng). No mouse received more than two doses of muIL-1β, and administration of muIL-1β doses was counter-balanced to eliminate potential order effects. Sleep-wake behavior was determined for 24 h after injections. ICV administration of muIL-1β increased in NREM sleep of both mouse strains in a dose-related fashion, but the maximal increase was of greater magnitude in C57Bl/6J mice. muIL-1β induced fever in C57Bl/6J mice but not in IL-6 KO mice. Collectively, these data demonstrate IL-6 is necessary for IL-1 to induce fever, but IL-6 is not necessary for IL-1 to alter NREM sleep.

show abstract

Telemetric Recording of Sleep and Home Cage Activity in Mice

Cited by 90 publications

References 0 publications

Brief Optogenetic Inhibition of the Basolateral Amygdala (BLA) in Mice Alters Effects of Stressful Experiences on Rapid Eye Movement Sleep (REM)

Brief Optogenetic Inhibition of the Basolateral Amygdala (BLA) in Mice Alters Effects of Stressful Experiences on Rapid Eye Movement Sleep (REM)

The Spectrum of Neurobehavioral Sequelae after Repetitive Mild Traumatic Brain Injury: A Novel Mouse Model of Chronic Traumatic Encephalopathy

Effects of i.c.v. administration of interleukin-1 on sleep and body temperature of interleukin-6-deficient mice

Contact Info

Product

Resources

About